... coenzyme. The Kmvalues for AdoCbi and Ado-Cbi-GDP were 26 ± 8 and 75 ± 28 lm, respectively, and the kcatvalues were (9.8 ± 1. 0) · 10 )3 Æs )1 and (4.5 ± 0. 8) · 10 )3 Æs )1 , respectively. In ... straightforward,separating AdoCbi and AdoCbi-GDP directly withoutfurther modification. In contrast, the reactant and prod-uct, AdoCbi and AdoCbi-GDP, were analyzed in theform of (CN)2Cbi and (CN)2Cbi-GDP, ... 100AAdoCbi-GDP (µM) Fig. 4. Binding of AdoCbl and its analogs toglutamate mutase by equilibrium dialysis.(A) AdoCbl, (B) MeCbi, (C) AdoCbi, and (D)AdoCbi-GDP. The proteins, 20 lM MutE and 100 lM...