... Characterization of the NADH:ubiquinone oxidoreductase
(complex I) in the trypanosomatid Phytomonas serpens
(Kinetoplastida)
Petra C
˘
erma
´
kova
´
1
, ... oligomer
of the small form. The different in uences of DPI on
the two forms could be caused by its failure to access
the flavin cofactor in the large form of complex I,
whereas the cofacto...
... N-terminal
residues of the catalytic domain are under-
lined. The preregion is indicated in red, the
pro-region in black, catalytic domain in blue
and the C-terminal domains in violet. The
assumed ... One or more of the disulfide bonds
is shown to be essential for maintaining the active
conformation of VPRK, since cleavage of the disul-
fides lead to inactivation...
... was
based on the conserved amino acid sequences of the
catalytic domain of members of the GH18 family
resulted in the amplification of an expected 147 bp
sequence. Cloning and sequencing of this fragment
revealed ... of the sole
Glyco_18 domain, the C-terminal tail of C. gigas CLPs
may not noticeably contribute to the structure and the
function of these protei...
... of alternative promoters in
DNASE1 results in no generation of protein isoforms.
The 5¢-UTR of eukaryotic mRNA in uences the initi-
ation step of protein synthesis and thereby in part
determines ... expression in vivo or
in vitro, including characterization of the promoter
region of the gene and the associated transcriptional
factors. Therefore, delineation...
... or by
FC-induced binding of 14-3-3s.
Results
The C-terminus of isoform 1 of the PM H
+
-ATPase
of A. thaliana (AHA1) interacts with the first 88 amino
acids of PPI1 [22], indicating that the PM ... suggested by
the parallel increase of the number of FC-binding
sites [18–20]. As to auxin, a soluble auxin receptor has
been reported to bind and activate the PM H
+
-ATP...
... of the genes. In
SNO1, the 199th G from the 5¢ terminus of the open reading
frame was deleted to result in a frame-shift and appearance
of stop codons in the downstream. As a result, a protein ... acetylpyridine adenine dinu-
cleotide (APAD) as cosubstrate [17]. The glutaminase
reaction was carried out in 0.3 mL of 50 m
M
Tris/HCl,
pH 8.0, in the presence of...
... and the novel Prm3 [25] relative to its
translational start site (ATG, designated +1) is
presented in Fig. 1. In order to gain further insights
into the modes of regulation of TPb, the aim of the
current ... Transcriptional activation of the human hemato-
poietic prostaglandin D synthase gene in megakaryo-
blastic cells. Roles of the oct-1 element in the
5¢-flan...
... localize the ligand
binding sites in the leukocyte b
2
integrins. The a chains of
the CD11/CD18 integrins contain an inserted approxi-
mately 200-amino-acid intervening domain (I domain),
which ... anti-ICAM-4
antibodies, indicate a major role for ICAM-4 in binding of
red cells to the I domains. The efficient inhibition of the
Fig. 10. Specific binding of purified recom...
... binding factors.
The introduction of mutation in one of the Ets-binding
sequences greatly decreased its promoter activity, suggesting
that the transcription of the a3 integrin gene in these cells ... including
Ets, GATA, and MyoD/E-box binding factors. The
introduction of mutation into one of the putative Ets-
binding sequences suppressed the promoter activity. In...
... suggested a single cut at
the C9–C10 double bond of the ring-bearing side of
3-OH-c-carotene in combination with several cleavage
options in the linear half of the molecule, namely at
the C9¢–C10¢,C7¢–C8¢ ... temperature of 340 °C, which was maintained for an addi-
tional 5 min. The He carrier gas flow was maintained at
1mLÆmin
)1
using a split flow of 1 : 20. The sp...