Báo cáo khoa học: The hinge region operates as a stability switch in cGMP-dependent protein kinase Ia doc

... enzyme as well. These ﬁndings support the concept that the hinge region of PKG acts as a stability switch. AbbreviationsMEW, maximal emission wavelength; PKA, cAMP-dependent protein kinase; PKG, cGMP-dependent ... by cGMPbinding is thought to induce the release of the auto-inhibitory domain from the active site, thereby activa-ting the kinase. This is indicated by a remarkableincrease in the proteolytic ... kDa [16]. In PKG Ia, trypsin cleaves preferentially at arginine77 (R77) of the hinge region, thereby eliminating the dimerization andauto-inhibitory domains [19]. Interestingly, the result-ing...

Tài liệu Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes docx

... A role of the cytoplasmic tail of membrane-spanning proteins in protein protein interactions has also been proved, e.g. the case of the association between the N-terminal region of the membrane-bound ... regulatedintramembrane proteolysis, can be released into the cytosol as a signalingfragment. The intracellular region of Jagged-1 may then exist in at leasttwo forms: as a membrane-tethered protein ... membrane-tethered protein located at the interface between the membrane and the cytoplasm, and the second is a soluble nucleocytoplas-mic protein. We expressed and puriﬁed a recombinant protein starting...

Báo cáo khoa học: "The clinical value of daily routine chest radiographs in a mixed medical–surgical intensive care unit is low"

... if an abnormality was present, whether it wasjudged to be an 'old' or 'new' finding). In case an abnormalitywas worsening, and fulfilling the criteria as in table 1, it wascategorized ... At present, in many ICUsCXRs are still routinely obtained on a daily basis, at least in The Netherlands [13].There may be advantages to eliminating daily routine CXRs.First, a routine strategy ... physician; and an 'old unexpected finding' was anyold finding not expected by the attending physician.If an important finding (as mentioned in Table 1) was found,then we determined...

Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efﬁciency doc

... wasdetermined using the Bradford assay with BSA as the standard [28].Phytase activity assayPhytase activity was determined by measuring the amountof phosphate released from InsP6using a modiﬁed ... determination of the phytase geneStrain HJB17 was cultured in Luria–Bertani medium at37 °C overnight and genomic DNA was extracted using the TIANamp Bacteria DNA kit (Tiangen, Beijing, China). The ... they can increase the amount of available phosphate by interacting together. Additionally,fusing PhyH-DI to a single-domain phytase appears to be an efﬁcient wayto improve the activity of the...

Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf

... anactivating element binding E2F1, -2 and -3. These acti-vating E2Fs cooperate with NF-Y proteins binding toCCAAT-boxes and with Myb proteins associating with a distal Myb site in activating the Cdc2 promoter.Binding ... Caenor-habditis elegans synMuvB proteins and is composed ofp130, E2F4 ⁄ 5 and DP1 ⁄ 2 and a module containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin-associated Lin-9 and Lin-53 ... ithas been shown that Lin-54 can bind to two sites in the Cdc2 promoter in vitro. Lin-54 binds in EMSAs to a region in the upstream part as well as to the CHR of the Cdc2 promoter [86].Cyclin A...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... participates in picolinicacid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, ... by the action ofACMS decarboxylase (ACMSD, also known as picoli-nate carboxylase; EC 4.1.1.45) into a- aminomuconicKeywordscerebral malaria; kynurenine pathway;metal-dependent amidohydrolase; ... selectiveenzyme inhibitors that may prove to be of potentialinterest to reduce life-threatening complications ofcerebral malaria, and as an important tool in validat-ing our proposal of hACMSD as a novel...

Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx

... with aA- andaB-crystallin declines. The altered interplay with othercrystallins illustrates that R116C aA-crystallin andR120G aB-crystallin, both observed in congenital cata-ract, maintain ... elementsand molecular chaperones. As a- crystallin chapero-ning capability declines, lens proteins are more likelyto aggregate, a characteristic linking cataract toother protein folding diseases ... proce-dural variation, recombinant adeno-associated virusvectors containing an extracellular superoxide dis-mutase (SOD) are administered by intramyocardialinjection, yielding long lasting protection...

Tài liệu Báo cáo khoa học: The tungsten-containing formate dehydrogenase from Methylobacterium extorquens AM1: Puriﬁcation and properties docx

... following standards:ferritin (440 kDa), catalase (232 kDa), aldolase (158 kDa),ovalbumin (43 kDa), and chymotrypsinogen (25 kDa).Peptide mass ﬁnger-printing was performed after trypsindigestion ... similar to the one in Eubacteriumacidaminophilum containing tupA are also found in Vibriocholerae, Campylobacter jejuni, Haloferax volcanii andMethanothermobacter thermautotrophicus, and a functionwas ... extorquens AM1 ﬁts well in the family of molybdopterin-containing FDH. All of the amino acid residues that havebeen shown to participate in the coordination of the centralMo/W atom via two pterin...

Báo cáo khoa học: The guanine nucleotide exchange factor RasGRF1 directly binds microtubules via DHPH2-mediated interaction docx

... S-transferase;LPA, lysophosphatidic acid; MAP, microtubule-associated protein; MAPK, mitogen-activated protein kinase; MBP, maltose-binding protein; PH, Pleckstrin homology domain.FEBS Journal ... that RasGRF1 bindsIB2 ⁄ JIP2 [33], a scaffold protein for the Jun N-ter-minal kinase signaling pathway. JIP proteins also link the motor proteins kinesins with the cargo complex tobe transported ... stathmin was added to the solutionand a slow decrease in the curve was observed, indicating partialmicrotubule depolymerization.Fig. 9. Sodium arsenate induces association of the N-terminal region...

Báo cáo khoa học: Identiﬁcation of NF1 as a silencer protein of the human adenine nucleotide translocase-2 gene pptx

... [13].Site-2 and Site-3 binding activity in all fractions wasmonitored by the in vitro DNase I protection assay. The DNA affinity column, used as the last step in the purification, was prepared with an ... wt, 5¢-TTTTGGATTGAAGCCAATATGATA-3¢;NF1mut,5¢-TTTTGGATTGAATAAAATATGATA-3¢;Site-2wt,5¢-GCGTCTCACCCTAGTCCTGGTCCTGCTCCAAGGGTTTTTGTCC-3¢;Site-2mut,5¢-GCGTCTCACCCTAGTAATGGTAATGCTCCAAGGGTTTTTGTCC-3¢;Site-3wt,5¢-GGGTTCTTTTGGCATCCCTGTAGC-3¢;Site-3mut,5¢-GGGTTCTTTTTAAATCCCTGTAGC-3¢.Chromatin ... Identification of NF1 as a silencer protein of the human adeninenucleotide translocase-2 genePeter Barath1,2, Daniela Poliakova1,2, Katarina Luciakova1,2and B. Dean Nelson11Department...

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