... proteins.a-Actinin–stFRET (A), a-actinin–GFP (B), filamin A–stFRET (C), filam- in A–CFP (D), spectrin–stFRET (E) and spectrin–CFP (F) in 3T3 fibro-blast cells; Collagen-19–stFRET (G) and COL-19–GFP (H) in C. ... stretching of the coiled-coil myosin II [44]. Schweiger et al. [45]obtained a three-phase force–distance relationship: alinear phase of $ 1mNÆm)1, a plateau of $ 25 pN, and a wormlike chain phase ... spectrin(Fig. 10E), filamin A (Fig. 10C), and a -actinin(Fig. 10A), and expression systems including HEK-293,3T3 and C. elegans, and the insertion locations wereoptimized to obtain protein distributions...