... purification and characterization of isoforms of
an extracellular serine glutamyl endopeptidase of
Enterococcus faecalis with different enzymatic activities.
J Bacteriol 187, 266–275.
30 Pavloff N, Pemberton ... presence of SDS.
Role of N-terminal Val69 in processing of the
GluV8 proform
Finally, the role of N-terminal Val69 of mature GluV8
was investigated. It has been pro...
... and
DcrH-Hr. Dv: DcrH-Hr fragment of DcrH from
D. vulgaris. Mc: McHr of M. capsulatus.
AB
Fig. 7. SDS ⁄ PAGE analysis of proteins during purification of McHr.
Samples from each step in the purification ... Lillehaug
Department of Molecular Biology, University of Bergen, Norway
Haemerythrin proteins comprise a family of O
2
-carrry-
ing proteins mainly found in a few phyla of...
... biochemical characterization of a
c-tocopherol methyltransferase (c-TMT) from Arabidopsis
thaliana and of a c-TMT purified from Capsicum annuum
fruits, a tissue with high accumulation of tocopherols. ... nutritional quality of crop plants [6]. Biosynthesis of
tocopherols was demonstrated in plastid envelopes [7] from
precursors originating from the plastidial isoprenoid pat...
... al.[12].
Purification and characterization of carboxypeptidases
from
H. armigera
larval gut extract
Gut extract was prepared from fourth instar lavae of
H. armigera as described previously [10]. Extract from 65
larvae ... and characterization of cDNAs encoding
H. armigera
carboxypeptidase
The construction of a cDNA library in the phage k vector
Lambda Uni-ZAP XR (Stratagene)...
... al. Membrane-bound prolyl oligopeptidase: mPOP
FEBS Journal 275 (2008) 4415–4427 ª 2008 The Authors Journal compilation ª 2008 FEBS 4427
Characterization of membrane-bound prolyl endopeptidase
from ... The
amounts of protein loaded onto gels were as follows: for the PSD-
95 blot, 70 lg of TM, 96 lg of LM, 89 lg of MM, and 93 lgof
HM; for the calnexin blot, 70 lg of T...
... 10
6
mL
)1
. Next, 0.5 mL of
propidium iodide staining solution (10 mg of propidium
iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of
NaCl ⁄ P
i
) was added to 0.5 mL of cell suspension. The ... spectra of extracted lipids (A) and PCA extracts (A¢) from one representative sample of MCF-7 cells; 2D
1
H NMR spectra
of extracted lipids (B) and PCA extracts (B¢) from o...
... gigas.
In this article, we report the characterization of a
new CLP, named Cg-Clp2, from the oyster C. gigas.
The tissue distribution and temporal pattern of expres-
sion of the gene encoding Cg-Clp2 ... conserved amino acid sequences of the
catalytic domain of members of the GH18 family
resulted in the amplification of an expected 147 bp
sequence. Cloning and sequencing of...
... number of transcripts containing exon 1a
with the number starting from exon 1. The abun-
dance of transcripts starting from exon 1 was 10-fold
higher than that of the transcripts starting from
exon ... 5¢-flanking
region of exon 1 in DNASE1. Deletion of the
upstream end of the 5¢-flanking region of exon 1 from
position )1386 to )231, )116, or )78 did not result in
any sign...
... are from one experiment, representative of
three giving similar results.
Fig. 6. Dependence on the concentration of PPI1
588
His
6
of the sti-
mulation of H
+
-ATPase activity in PM purified from ... the mechanism of action of PPI1.
The analysis of the pH dependence of PPI1-
induced activation of the H
+
-ATPase showed that
stimulation decreases dramatically with the incr...
... Proteinase K from
Tritirachium album Limber. Characterization of the
chromosomal gene and expression of the cDNA in
Escherichia coli. Eur J Biochem 179, 185–194.
Characterization of a Serratia ... Isolation of genomic DNA from Serratia sp.
Genomic DNA was isolated as described by Chen and Kuo
[38] for use in identification of the peptidase gene.
Generation of an 200-bp fr...