Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... E552 in NBD1 and E1197 in NBD2. Tofurther characterize the role of these residues in catalysis, we created in Abcb1a the single-site mutants E552D, N and A in NBD1, and E1197D,N and A in NBD2, ... further the role of the IC in the catalysis of ABC transporters, we created, in Abcb1a, six single-site mutants (E552D, N and A, and E1197D, N and A,) and three double-mutants(E552Q ⁄ E1197Q, E552Q ... of the trypsin-sensitiveregion situated in the linker domain of Abcb1a. Fol-lowing trapping in the absence or presence of Vi and mild-trypsin treatment, the trypsin degradation pro-ducts of...
- 13
- 458
- 0
Báo cáo khoa học: G protein-coupled receptor 30 down-regulates cofactor expression and interferes with the transcriptional activity of glucocorticoid pdf
... to date concerns the role of GPR in enhancing cell proliferation [16–20]. There arealso examples suggesting t he antiproliferative activity of GPRs [21–23]. The role of GPR30 in glucocorticoid-mediated ... discrimination of cells differing in their sensitivityto steroids is essential in diseases involving resistance tosteroid h ormone treatment. In the present study, weaddressed the question of the ... preventsERa-induced and ligand-induced activation of the promo-ter 3xERE-TATA [9]. Differential expression of cofactorshas been proposed in explanation of some of the differentialeffects of s teroid...
- 10
- 389
- 0
Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx
... inhibitgrowth of the strain even in the presence of IPTGinduction. All of these results suggest that the low geneexpression associated with NGG codons in the down-stream region following the initiation ... paus-ing at early NGG codons is the reason for the observed low gene expression. The length of the peptidyl moiety of the peptidyl-tRNA in uences the drop-off process in the case of very small mini-genes ... other analysed argininecodons AGA, CGC, CGA and CGU. The amino acidsequence of the nascent peptide is the same in all of these cases. This fact eliminates any amino acid contri-bution by the...
- 11
- 510
- 0
Tài liệu Báo cáo khoa học: A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3 pptx
... at the interface between the a and b subunits.A comparison of the amino-acid sequences showed that the ADP-bindingmotif in the a subunit of PDH1 clearly differs from that in the a subunit of ... stream from the 5¢-terminus of the predic-ted ORF, is the proper initial codon for a2. The N-ter-minal amino-acid sequences of the b2 and c2 subunitscorresponded to the sequences of the predictedPH1751 ... amino-acidalignment of the a and b subunits of PDH1, PDH2 and T. profundus dye-l-proDH. The amino-acid sequence of the a1 subunit of PDH1 showed 31% and 32% identi-ties with the a2 subunit of PDH2 and the a...
- 11
- 549
- 0
Báo cáo khoa học: Activated transglutaminase from Streptomyces mobaraensis is processed by a tripeptidyl aminopeptidase in the final step pptx
... Ne-(c-glutamyl)lysine bonds, the incorpor-ation of polyamines into proteins, the deamidation of protein-bound glutamines, and the covalent attachment of proteins to lipids such as x-hydroxyceramides ... peptides used exhibited a pNAresidue on the C-side. The already slight yellowing of the solution indicated SM-TAP activity against the compound in the incubation mixture.SM-TAP has a clear preference ... TAMEPreleasing a peptide of 41 amino acids. P14may inhibit the reaction byattaching to TAMEP in equimolar concentrations [1]. FRAP-TGaseexhibits the full activity of the mature enzyme [1]. Final...
- 7
- 480
- 0
Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt
... adaptation of RCC cells to the lack of calbindin protective function. The protection from apoptosis despite the lack of cal-bindin could be explained by the decrease in TRPC4,or by retention of the ... expansion.Re-expression of such inhibitors in angiogenic tumorsimpedes their progression: these include angiostatin,endostatin, tumstatin, PEDF, SPARC (secreted pro-tein, acidic and rich in cysteine), tissue inhibitor ... protein misfolding, which, in turn, wascaused by changes in calcium metabolism. Calciumintake in response to growth stimuli was reducedbecause of the decrease in TRPC4 and the lack of calbindin....
- 13
- 609
- 0
Tài liệu Báo cáo khoa học: A possible role of mitochondria in the apoptotic-like programmed nuclear death of Tetrahymena thermophila Takashi Kobayashi and Hiroshi Endoh docx
... (Figs 1 and 3). According to the observation made by Lu and Wolfe [23], who used acombination strategy of 4,6-diamino-2-phenylindole(DAPI) staining for the detection of DNA and Azodye staining ... becomes acidic in the final step of PND [22,23]. DePsipher staining of the macronucleus appeared initially during the stage of autophagosome formation, and persisted until resorp-tion of the parental ... even in 1 mm of Mg2+(comparelane 2 and 3 in Fig. 4C), indicating a different naturefrom most other DNases. On the other hand, nicking activity was unaffected by Mg2+, as shown by the increased...
- 10
- 642
- 0
Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx
... noteworthy in view of their coordinative potential, CeMT1 includesfour histidines, whereas CeMT2 only has a terminalone. In the absence of a comprehensive analysis of the metal-binding abilities of ... coordination of the Zn(II)ion bound at the C-terminal moiety of CeMT1 is con-tributed to by histidines, and the number of theseinvolved in metal binding is analyzed below.Thus, the overall ... Ka¨gi (Institute of Biochemistry of the University of Zurich, Switzerland) and also used as a template for the construction of regionscoding for the deletion mutant CeDHis and the independentN-terminal...
- 17
- 600
- 0
Báo cáo khoa học: Alternative splicing: role of pseudoexons in human disease and potential therapeutic strategies pot
... ItalyIntroductionTowards the end of the 1970s, in the beginning of pre-mRNA splicing research [1,2], defining exons and introns was essentially based on observing the finalcomposition of the mature ... splic-ing pattern by steric hindrance of the recruitment of the splicing factors to the targeted splicing competentcis-elements, thus forcing the machinery to use the nat-ural sites. Dominski and ... J (2006) A splice-supporting intronic mutation in the last bp position of a cryptic exon within intron 6 of the CYBB geneinduces its incorporation into the mRNA causingchronic granulomatous...
- 15
- 467
- 0
Báo cáo khoa học: Gene expression silencing with ‘specific’ small interfering RNA goes beyond specificity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx
... expected following the downregulation of PCNA, coding for a protein involved in the control of DNA replication and CDK2-cyclin A activity [15]. In most cases (about 70%), and as expected,DF-induced ... differences in exposure times during western blot-ting. We also observed a slight increase in SREBF1protein level triggered in the presence of the NTsiRNA, in agreement with the slight increase in SREBF1 ... into account in the normalization.Furthermore, in order to consider the purity and quality of the mRNA, a second normalization step was performed on the basis of the average of fluorescence intensities...
- 16
- 494
- 0
Từ khóa: Báo cáo quy trình mua hàng CT CP Công Nghệ NPVGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIHIỆU QUẢ CỦA MÔ HÌNH XỬ LÝ BÙN HOẠT TÍNH BẰNG KIỀMTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ