... have been interested in the molecularbasis of substrate activation in wild-type and mutants of human BuChE [11,15,16], as well as substrate activation in wild-type and mutants of human and Bungarus ... Protein Sci. 6,130 2–1 307.32. Main, A.R. (1979) Mode of action of anticholinesterases. Phar-macol. Ther. 6, 57 9–6 28.33. Fersht, A. (1999) Structure and Mechanism in Protein Science,pp. 17 2–1 73. ... oxyanion hole, ap-cation-binding site, and an acyl-binding pocket) are thesame in AChE and BuChE (Fig. 1). The acyl-bindingpocket, which is responsible for the difference in substratespecificity...