Báo cáo khoa học: Gene expression silencing with ‘speciﬁc’ small interfering RNA goes beyond speciﬁcity – a study of key parameters to take into account in the onset of small interfering RNA off-target effects potx

... such as small interfering RNAs(siRNAs) or microRNAs, leading to the degradation of a particular mRNA (slicing) or to repression of translation [2,3]. Thereafter, the use of chemicallysynthesized ... cell transfection with siRNAs, total RNA wasextracted using the Total RNAgent extraction kit (Pro-mega, Madison, WI, USA). mRNA contained in 5 lgoftotal RNA was reverse transcribed using SuperScript ... normalization and statistical analysis The data were normalized in a two-step procedure. First, a correction was applied using a factor calculated from the intensity ratios of internal standards in the...

Báo cáo khoa học: Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana ppt

... 5¢-ATCGACGGGCCTGACTCAT-3¢,5¢-CAACATTGAGCCCAGCAATAAC-3¢,5¢-CAGCTATTTAAGCCGTCTTTTCCA-3¢,5¢-GATAGATGCAGAGCCACCAAAGA-3¢,5¢-CGGACATGAGAGAGCAAAGTCA-3¢,5¢-CAGCTGCAAATTATGGTGAAG-3¢ and 5¢-ACCCGACGGTGGTGACTACA-3¢, were ... RT-PCR with a forward primer, 5¢-ACGTCGCTGCAGCGATCTGATCACTGAGAAAC-3¢, and a reverse primer, 5¢-AAAGCCGGTACCCTCTGCTATTACAATGACGAAAACGATTATC-3¢, using mRNA from Arabidopsis plantlets treated with ... 5¢-VIC-CAGTACCTTCCAGCAGATGTGGATCGC-TAMRA-3¢,5¢-FAM-CCAGCTTACTTACTTCAATGATGCTCAAAGGC-TAMRA-3¢,5¢-FAM-CTATGCAAGGTCTCAGTCAGGCTCGGC-TAMRA-3¢,5¢-FAM-ATGCTAATGTGGCTCCAGTTTGCCTCT-TAMRA-3¢,5¢-FAM-TCCACTCTCTTTTGAGCCATCCAATGC-TAMRA-3¢,5¢-FAM-AACGACTTGCTTTTGCTCTTCTCTCGC-TAMRA-3¢...

Báo cáo khoa học: Gene expression waves Cell cycle independent collective dynamics in cultured cells doc

... idea of the amount of vari-ation at the single gene level explained by the abovedescribed mode, we calculated a scale independentindex of the range of variation of each of the 7160ORFs in the ... by theirmutual correlation with pc1rand, thus indicating anaspeciﬁc (from the point of view of the biological role of the involved genes) character of the extracted mode. In order to obtain an ... independence of each cell of the ensemble from all the others, is a necessary prerequisite to attach single cellbased explanations to the grand averages taken from population data. Thiswas the prevailing...

Báo cáo khoa học: Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a speciﬁc ternary complex with tRNASer potx

... general RNA bindingdomain appended to plant methionyl-tRNA synthetaseacts as a cis-acting cofactor for aminoacylation. EMBOJ 19, 690 8–6 917.38 Kaminska M, Shalak V & Mirande M (2001) The appended ... possess a tRNA-binding motif, but it instead participates in the formation of a speciﬁc ternary complex with seryl-tRNA synthetaseand tRNASer, strengthening the interaction of seryl-tRNA synthetase ... Croatia2 Department of Microbiology, The Ohio State University, Columbus, OH, USAAccurate aminoacylation of tRNA by aminoacyl-tRNAsynthetases (aaRSs) is a crucial step in the faithfultranslation...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... reintroducing the nrdF gene into the geneticbackground of corynebacteria comprised an initialtransfer into the accessible species C. glutamicum and the performance of a second, ﬁnal gene transfer into C. ... cannot,however, explain the remarkably broad wings of the signal. The broad lineshape and the enhanced relaxa-tion properties of the signal at 77 K indicate that the YÆ is coupled to a paramagnetic ... of the tyrosyl- and manganese-containing small subunit. Here, the reaction was started by addition of CDP. Data are given as the mean of triplicates.Inhibitory effects of the synthetic heptapeptide...

Tài liệu Báo cáo khoa học: Gene silencing at the nuclear periphery pdf

... components of the nuclearlamina shown to interact with transcriptional regula-tors include LAP 2a, a nucleoplasmic LAP2 isoform,and lamin A ⁄ C [5 4–5 6]. LAP 2a was shown to complex with lamin A ⁄ C and ... cardiomyopathy [71]. Two NPC pro-teins, ALADIN (also termed Adracalin or AAAS) andnup62, can be added to the expanding list of mutatednucler lamina proteins causing these diseases. Muta-tions in the ... clusters at the nuclear periphery adjacent to the nuclear lamina, hinting that proteins of the lamina may participate in regulation of gene expression. Recent studies on the molecular mechanismsinvolved...

Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... 5¢-GGTTATCATATAAAGATCTCAAATTACCC-3¢, for the second PCR the primers were: 5¢ primer, 5¢-GGGTAATTTGAGATCTTTATATGATAACC-3¢ and 3¢ primer, 5¢-CGCGCGGGATCCTTAGTGATGGTGATGGTGATGGGTGACCGGTTTTTTGGTAGGTGAAC-3¢.ThethirdPCRwascarried ... molecules in the treatment of several pathologies and because of the potential use of the toxins as biological weapons. Alteration of their MHC and TCR binding capacity by site directedmutagenesis ... ArgentinaSuperantigens (SAgs) are a class of disease-causing andimmunostimulatory p roteins o f b acterial or viral o rigin t hatactivate a large number of T-cells through interaction with the Vb domain of T-cell...

Báo cáo khoa học: SOX10, in combination with Sp1, regulates the endothelin receptor type B gene in human melanocyte lineage cells pptx

... mapping analysis of EDNRB transcripts.An autoradiogram of the S1 nuclease mapping analysis is shown. Total RNA was prepared from the indicated cell lines at the top of panel. The S1 probe and ... 5¢-CCCAAGCTTATGGCGGAGGAGCAGGATCTATCGGAGGTG-3¢ (sense) and 5¢-GCTCTAGATGAGGTGGGCAAGGAACAGGGCACACAGGCT-3¢ (anti-sense); and GAPDH: 5¢-ACCACAGTCCATGCCATCAC3¢ (sense) and 5¢-TCCACCACCCTGTTGCTGTA-3¢ ... 679 5–6 802.53 Udono T, Yasumoto K, Takeda K, Amae S, WatanabeK, Saito H, Fuse N, Tachibana M, Takahashi K,Tamai M et al. (2000) Structural organization of the human microphthalmia-associated...

Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

... anarrow and asterix. The relative mobility of biotin-labelled RNA ladder (L) is indicated to the left of each blot. A 1 lgaliquotofeachRNAsampleshownin(C)and(D)wassubjectedtoRPAusingtheb-actin-speciﬁc ... stability of CB1 mRNA was affected in a mannerthat was dependent on the length of the CAG repeat andrelative expression of the human HD transgene in the twostrains of mice and that mutant huntingtin ... in the R6/2 striataland c ortical RNA samples a nd in wild-type cortical RNA samples compared to wild-type striatal RNA samples,although i t appeared that the relative proportion of eachband...

Báo cáo khoa học: Gene cloning, expression and characterization of avian cathelicidin orthologs, Cc-CATHs, fromCoturnix coturnix pdf

... C G ATGA acg-t c 480Cc-CATH1 actgtcccctcgctgccttccatccaataaaggtctttgctggtaaaaaaaaaaaaaaaa 531Cc-CATH2 TTG-CTGAg-gaataaa-ggggc gtgtg c-accaagc -a 517Cc-CATH3 g tc a cc c aataaa-c-g ttca-gct ... SignalP ⁄ ) indicates a 17 amino acid signal peptide located at the N-termi-nus. Noticeably, four cysteines that are conserved in the cathelin domain of all cathelicidins identiﬁed to date are also ... samples was dispensed into a 96-wellmicrotiter plate and mixed with 50 lL of bacteria or yeastinoculums in MH. Human cathelicidin LL-37 (withoutNaCl), ampicillin and kanamycin was used as a...

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