Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspeciﬁc alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx

... hypophosphatasia with tissue-nonspeciﬁc alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain Natsuko Numa1, Yoko ... characterized by reducedserum alkaline phosphatase levels and defective mineralization of hard tis-sues. A replacement of valine with alanine at position 406, located in the crown domain of ... hypophosphatasia, (b) infantile hypophos-phatasia, (c) childhood hypophosphatasia, (d) adult hypophosphatasia and (e) odonto hypophosphatasia [1–4]. Perinatal and infantile forms of hypophosphata-sia are...

Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc

... AMPApathway), referred to as the GOX pathway.(B) Reaction catalyzed by GO on glyphosate,an alternative to the AMPA pathway ascatalyzed by GOX.Mechanisms of glyphosate resistance L. Pollegioni et al.2758 ... mostglyphosate-resistant biotypes. In the case of Conyzacanadensis, glyphosate accumulates in vacuoles of resis-tant plants at a markedly faster rate than in sensitiveplants [61]. Analysis of the transcriptome ... of glyphosate degradation are known.Top: cleavage of the carbon–phosphorusbond, yielding phosphate and sarcosine (the C-P lyase pathway). Bottom: cleavage toyield AMPA and glyoxylate (the AMPApathway),...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... Koike Y, Nohata J,Kawasaki H, Kadono-Okuda K, Yamamoto K,Suzuki MG, Shimada T et al. (2003) The construction of an EST database for Bombyx mori andits application. Proc Natl Acad Sci USA 100, 14121–14126.27 ... Cleavage of structural proteinsduring assembly of head of bacteriophage-T4. Nature227, 680–685.31 Daimon T, Katsuma S, Iwanaga M, Kang W &Shimada T (2005) The BmChi-h gene, a bacterial-typechitinase ... with the sku loci(Fig. 3 and Table 1) and dramatically decreased the enzymatic activity (Fig. 6). These genetic and biochem-ical data, along with previous observations that the sku mutant accumulates...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... 5¢-GAGCCAACAGAAGTTTGCTTCACACGTTGTTGAGAAATGTTT-3¢ (forward) and5¢-GTCAAACATTTCTCAACAACGTGTGAAGCAAACTTCTGTTGG-3¢ (reverse) to APUM-2 and 5¢-CGATGCAGAAATTCAGTAGCAACATGGTGGAACGATGTCTCA-3¢ (forward) and 5¢-GCATGAGACATCGTTCCACCATGTTGCTACTGAATTTCTGCA-3¢ ... cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUA At4 g36040 (1) Protein containing DNAJ domain; unknown functioncuacgucggacggaacugggaaaccgaucaguguugguagugaguuaacucggugaccgaguuaguagaacgaguuaauuagUGUAAAUAcgaagcca At4 g39090 ... containing PHD domain; unknown functionugcgucugacaUGUACAGCcccugccaaauuuuaauaggcaatAGUAAAUAaauaacgacaagaagcaaaugg At5 g24490 (1) Ribosomal protein; unknown function cucaucucuccuuacaguuuaccuguguaggaguuaggguucuugaauaaacaaugcaacaaagauuguagaagucagUGUACAUA At4 g36040...

Tài liệu Báo cáo khoa học: Molecular cloning and characterization of soybean protein disulﬁde isomerase family proteins with nonclassic active center motifs pdf

... RT-PCR with a Thermal Cycler Dice RealTime System (TaKaRa Bio Inc.). Forward primers 5¢-CGTTTGAAGGGTGAGGAGGAAAA[FAM]G-3¢ and 5¢-CACAAGAGAGTTCTGCGATAACCTTG[FAM]G-3¢ (Invi-trogen Corporation) and ... reverse primers 5¢-AAGTAGGCAACAAAACAACG-3¢ and 5¢-GTTTTCCCGACAATAA-CATGG-3¢ were used for detecting GmPDIL- 3a andGmPDIL-3b, respectively. Primers for quantiﬁcation of actin mRNA were as described ... protocol. mRNAwas isolated from total RNA with the PolyATtract mRNAIsolation System (Promega Corporation, Madison, WI,USA). The 3¢-RACE method was performed using the SMART RACE cDNA Ampliﬁcation...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... (Table 3). Secondary antibodies(anti-rat IgG, anti-guinea pig IgG and anti-rabbit IgG; allraised in goat and conjugated to alkaline- phosphatase; Sigma, Saint Louis, MO, USA) were used at 1 : 2000 ... proﬁle of an acetic acid extract of 30 lobster sinusglands. Only the part of the chromatogram where CHHs and VIHsare eluted is shown. The nature of the ultraviolet absorbance peakswas assessed by ... chromatograms from different sinus gland batchesshows a constant abundance ratio of the different pep-tides, with the VIH I peak being half the size of the VIH II peak (Fig. 1). The rationale of...

Tài liệu Báo cáo khoa học: Molecular determinants of ligand speciﬁcity in family 11 carbohydrate binding modules – an NMR, X-ray crystallography and computational chemistry approach doc

... the Verlet leap-frog algorithm and the nonbonded interactions truncated with a 10 A ˚cutoff. The temperature of the system was reg-ulated by the Langevin thermostat to maintain the tempera-ture ... carbohydrate conformation. Proc Natl AcadSci U S A 98, 10541–10545.35 Basma M, Sundara S, Calgan D, Venali T & Woods RJ(2001) Solvated ensemble averaging in the calculation of partial atomic ... that comprises a at platform of the CBMand in the type of interactions between the carbohy-drates and CtCBM11. Regardless of the CBM, gener-ally, we have found that the central carbohydrateinteracts...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... dependent on activation of the extracellu-lar regulated kinase MAPK. The activation of MAPKis also important in regulating the RA FLS cytoskeletalstructure and migration by fractalkine ⁄ CX3CL1 ... Immunolocalizationanalysis indicated that IP-10 ⁄ CXCL10 is associatedmainly with inﬁltrating macrophage-like cells andﬁbroblast-like cells in the RA synovium, and the inter-action of activated ... N,Kamogawa J, Yamamoto H, Takubo N, Nakata S,Yamada K, Yamamoto S et al. (2005) Accumulation of plasma cells expressing CXCR3 in the synovial sublin-ing regions of early rheumatoid arthritis in associationwith...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of environmental factors pdf

... factor-jB and extracellular signal-regu-lated kinase signaling cascades. In addition, AhRexpression in synovial cells was upregulated by TNF -a. These data suggest that TNF -a activates AhR expres-sion ... pregnancyare at the greatest risk of developing RA [45]. Theseresearchers also suggested that the association amongbreastfeeding, pregnancy and RA may be related toeither increased prolactin ... RA patients andcompared RA disease activity between RA patients with JCP and those without JCP using a large observationalcohort study of RA patients [The 6th Japanese version of the IORRA...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt

... we also found that SAA stimulationpromoted nuclear translocation of NF-jB, whereaspre-incubation of SAA with RAGE inhibited nucleartranslocation [12]. These data suggested that SAA of RA joints ... colony-stimulating factor.RANKL activates the TNF receptor-associated factor6, c-Fos, and calcium signaling pathways, all of which are indispensable for the induction and activa-tion of NFAT1. NFAT1 ... with fenoﬁbratemay serve as a new anti-NF-jB strategy for the treat-ment of RA. We have also shown, by case reports,that fenoﬁbrate is useful for the treatment of RA andautoimmune hepatitis [19,20].Besides...

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