... body, gut of mated queens and abdomen of adult males were ana-lyzed by western blotting. To compare receptor expressionbetween virgin and mated queens (Figs 2–4), membranes of four pairs of ovaries ... contained 2 lL of thediluted cDNA (1 : 2), 0.4 lm of each primer, 400 lm of dNTPs, 1 · reaction buffer and 0.4 lL Taq DA polymerasein a final volume of 20 lL. PCR amplification of VgRproduct ... ovarian-specific expression of SiVgR, membrane fractions of different tissues takenfrom mated queens were analyzed by western blot(Fig. 1). A band was recognized by the SiVgR antiseraonly in...