... the
higher inhibitory activity of the cEL peptide and the very
low inhibitory activity of the cYT peptide compared to
other peptides.
The flanking residue of the b-turn, i.e. Tyr3 of the cVY
peptide ... chain
topologies of the turn region mimic those of rat CD2.
Structure of cyclic hexapeptides – cEL peptide. The
chemical shifts of amide resonances of peptide cEL were
wel...
... instead of glycopeptides [8].
For detection of IgE reactivity, streptavidin conjugated with
horseradish peroxidase instead of alkaline phosphatase was
used. After visualization of the enzymatic activity ... subtraction
of the spontaneous release of the basophils, the allergen-
induced histamine release was calculated as percent of the
total amount of histamine determined aft...
... added.
Endotoxin activity determination by the chromogenic
Limulus test
Endotoxin activity of the glycolipids was determined by a
quantitative kinetic assay [27] based on the reactivity of
Gram-negative ... of the horseshoe crab Limulus polyphemus,to
form a solid gel in the presence of minute amounts of
endotoxins. The comparison of LPS and MfGl-II in the
LAL assay shows, as...
... rotation of
the bTD module at the main chain of D606 to dock it
against the calf-2 domain with its C-terminus in prox-
imity of the N-terminus of the b
3
‘open’ TM helix.
Overall evaluation of the ... (involving the
swing-out of the b
3
hybrid domain [6]) taking place
without the requirement of full separation of the TM
helices. Since then, crystallographic structures of the...
... AAA activity of BuChE
in plasma and tissues could participate in the metabo-
lism of these aryl acylamide drugs and xenobiotics.
However, the potential detoxification role of the AAA
activity of ... effects of these
compounds on the AAA and esterase activities of
human BuChE.
The concentrations of tyramine and serotonin that
activate or inhibit the AAA activity of BuChE (...
... constant of
the 5¢ exon mimic, h is the fraction of 5¢ exon mimic bound
to the ribozyme, and [ribozyme]
u
is the concentration of
unbound ribozyme in the reaction.
Measurement of rate constant of
substrate-cleavage ... property of nuclear
group I introns. RNA 9, 1464–1475.
6 Herschlag D & Cech TR (1990) Catalysis of RNA
cleavage by the Tetrahymena thermophila ribozyme. 1.
K...
... FEBS
potential regulates lateral distribution of K-Ras after it
binds to membrane.
The third component of membrane potential, elec-
trostatic membrane surface potential, is a consequence
of incomplete ... of cytoplasmic composition of anionic li-
pids in membrane binding of K-Ras was evaluated by
studying the effect of ATP depletion, which inhibits
inward movement of PS, with...
... a twofold molar excess of papain.
The residual activity of papain was measured with Z-FR-AMC. Rel-
ative activity of cystatin F is shown, from 0% (uninhibited enzyme)
to 100% (the lowest activity ... localization
of cystatin F, its restricted and readily regulated expres-
sion [18], selective and not too potent inhibition of
cathepsins are all in favor of the active role of...
... determine the kinetic para-
meters of chitinase A.
Having established confidence in the validity of the
method, we systematically investigated, by using ESI
MS, the kinetic properties of chitinase ... quantities of protein util-
izing the principle of protein-dye binding. Anal Biochem
72, 248–254.
33 Laemmli UK (1970) Cleavage of structural proteins dur-
ing the assembly of...
... and absence of 4 lm ChlH; this concentration of
ChlH gives almost maximal stimulation of methyltrans-
ferase activity. 0.2 lm ChlM was assayed with 1 mm
SAM and various concentrations of MgD. Figure ... the
methyl carbon of SAM, or the enhancement of the
negative charge on the propionate carboxyl groups of
MgD. The rate constants quoted in Scheme 1 are all
Table 1. Summary of...