... generate the
monomeric form of the CP. Furthermore, we have shown that residue F13
is critical for self-assembly of the CP subunits into NLPs. The replacement
of F13 with hydrophobic residues ... led to formation of either NLPs (F13Y and
F13L) or monomeric forms of the protein (F13G,
F13A, F13R, F13E, F13S), which were always
detrimental to accumulation of...
... primer for construction of truncated
AmyQ variants for synthesis of nascent chains
amyQ95 GCCGGATCCTTCTCCTAAATCATACAA Amplification primer for construction of truncated
AmyQ variants for synthesis of ... around the filter, which can be visualized by staining
of the plates with iodine vapor. The radius of the resulting
halos is indicative for the amounts of ac...
... January
2011)
doi:10.1111/j.1742-4658.2011.08018.x
NOX is the catalytic subunit of NADPH oxidase, the superoxide-generat-
ing enzyme. Among several isoforms of NOX, NOX4 is abundantly
expressed in various tissues. To clarify the mechanisms of ... dramatically decreased
by the deletion of GC-box1. The activity was further
decreased by the deletion of GC-box2 (Fig. 3...
... was then further
used in POS tagging in (Ng and Low, 2004). One
main disadvantage of this model is the difficulty in
incorporating the whole word information.
The second kind of solution is the ... Seg
C
and
SegTag
L
are the predictions of the three coarse-
grained solvers. For the three words at the begin-
ning and the two words at the end, the three predic-...
... triggers the formation of reac-
tive oxygen species, the disruption of electron transport and
energy metabolism, and the release of caspase-activating
proteins, c ytochrome c, a nd apoptosis-inducing ... residue within the kinase domain is
replaced by an alanine residue, is inactive. The death-
promoting function of DAP kinase requires t he kinase
activity; therefore...
... requires
neither Tyr residue for efficient turnover by TOP. This
distinction among substrates has allowed for a careful
analysis of the role of the conserved Gly residues in
the 599–611 loop. The flexibility ... the flexible loop
region of TOP is responsible for this enzyme’s posi-
tioning of substrates for catalysis [15]. The present
results clarify the prima...
... signal is impaired.
We finally examined the effect of deletion of the
components of the PDZ binding motif on the signal-
ing properties of the receptor in Ca
2+
imaging
experiments. Truncation of the ... and have elucidated the molecular
details of this interaction.
The primary source of olfactory signaling and the
sites of expression of ORs are the cil...
... during the f orced
molecular dynamics calculated with the torque of 56 pNÆnm. The
secondary structure of c is shown for the time of 1 ns (the end of initial
equilibration), 17 ns (half of turnover), ... main axis of the shaft. The ‘bearing’ included a
total of 138 residues from the neighbouring portion of (ab)
3
located within 1.8 nm from the rotary axis (...
... given in Fig. S2.
The regioselectivity of CYP77A4 was determined on the
basis of the peak area of each metabolite detected by GC.
Metabolites of oleic acid
For the analysis of the products generated ... S4. The regioselectivity of CYP77A4 was
determined on the basis of the peak area of each metabolite
detected by GC.
Metabolites of linoleic acid
For the...
... b-sheet A to form a latent conformer [16].
However, this is unlikely as the latent conformer of the
serpins is unable to form polymers [17,18]. Other charac-
teristics of the latent conformer are ... of Ser52Arg neuroserpin is similar to that
of the polymeric conformation in which b-sheet A is filled
with the reactive loop of another neuroserpin molecule.
Moreover t...