Báo cáo khoa học: Replacement of two invariant serine residues in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity docx

... in chorismate synthase provides evidence that a proton relay system is essential for intermediate formation and catalytic activity Gernot Rauch1, Heidemarie Ehammer1, Stephen Bornemann2 and ... Neurospora crassa chorismate synthase with alanine, producing two single-mutant proteins(Ser16Ala and Ser127Ala) and a double-mutant protein (Ser16Ala-Ser127Ala). The residual activity of the ... such a gating mechanism. In summary, our data provide evi-dence that the two invariant serine residues arerequired to organize a chain of water molecules in theactive site of chorismate synthase, ...

Tài liệu Báo cáo khoa học: Myristoylation of the dual-speciﬁcity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf

... Several DSPs have been establishedas MAPK phosphatases that dephosphorylate the Tyr and Thr residues in the activation loop of MAPKs and thereby attenuate signaling [17,19]. In addition, there is a ... JSP1 was myristoylated at itsN-terminus. (A) Schematic representation of JSP1. The potential myristoylation site(Gly2) is indicated by an asterisk. Aminoacids critical for catalysis (D57 and ... proteins that arepart of the MAPK signaling modules. Although pro-tein phosphatases have long been viewed as negativeregulators that terminate MAPK signaling, it is nowevident that they play an important...

Tài liệu Báo cáo khoa học: Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions doc

... generation. Theresulting serum was immunoaffinity purified and tested for reaction against HydA1 and HydA2 overexpressed in E. coli and against HydA2 in anaerobically induced algalextracts.Algal ... byPCR in 50 lL reactions that contained 1 lLofKODHOTSTART polymerase (Novagen, Madison, WI, USA),25 lL of BAC clone cell-free lysate as template, 125 ngeach of forward (5¢-CTGGACGTGACAAACAAGACCC-3¢, ... days.cDNA library screening Two specific primers, BE5P1 (5¢-AACATCTTCAAGGAGCGTGGCATC-3¢)andBE3P1(5¢-AGACAGCAGGAGACTCACAATCAC-3¢), were used to amplify a C. rein-hardtii expressed sequence tag (EST),...

Báo cáo khoa học: Cooperation of two carotene desaturases in the production of lycopene in Myxococcus xanthus pot

... strain FD6. This promoterwas generated by hybridization of two complementary oli-gonucleotides, Part-1 (5¢-AGCTTGACAGGCCGGAATATTTCCCTATAATGCGCTGCA-3¢) and Part-2 (5¢-GCGCATTATAGGGAAATATTCCGGCCTGTCA-3¢), ... production in the absence of CrtIb, and instead it leads to theaccumulation of the intermediates phytoﬂuene and f-carotene in decreasing amounts. On the other hand,CrtIb is itself incapable of introducing ... two complementary oligonucleotides,Part-5 (5¢-CTAGATTGACAGGCCGGAATATTTCCCTATAATGCGCAT-3¢) and Part-6 (5¢-CGATGCGCATTATAGGGAAATATTCCGGCCTGTCAAT-3¢), which con-tain, like the Part-1-2 promoter,...

Báo cáo khoa học: Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes pptx

... species and transgenic plant lines. Acyl composition in seeds from C. alpina, C. palaestina and wild type and fad2 mutants of nontransforme d A. thaliana and transformed with either the C. alpina D12 ... 12(E )and1 2(Z) isomers of 18:2. The catalytic activity of the C. alpina acetylen aseenzyme is compared with that of the evolutionarilyclosely related Crepis palaestina epoxygenase and another,evolutionarily ... thaliana and Saccharomyces cerevisiae.The acetylenase from C. alpina D12 desaturated both oleate and linoleate with about equal eﬃciency. The d esaturation of oleate gave rise to 9(Z),12(E)- and...

Báo cáo khoa học: Replacement of helix 1¢ enhances the lipid binding activity of apoE3 N-terminal domain pot

... Edmonton, CanadaHuman apolipoprotein E (apoE) is comprised of two structural domains, a 22 kDa N-terminal (NT) domain and a 10 kDa C-terminal (CT) domain [1,2]. Studiesconducted with isolated domains ... increased lipid binding activity of HTapoE3-NT is maintained in the context of full-lengthapoE. It is conceivable that an NT domain withincreased lipid binding activity will result in a greaterproportion ... conformation, a lipid binding induced conformational adapta-tion is required for manifestation of LDLR binding ability. To investigatethe structural basis for this conformational change, the short...

Báo cáo khoa học: Cloning of two melanocortin (MC) receptors in spiny dogfish MC3 receptor in cartilaginous fish shows high affinity to ACTH-derived peptides while it has lower preference toc-MSH ppt

... compared against databaseassemblies usingBLASTN and BLASTX.Screening of a phage genomic library and isolation of full-length geneThe spiny do gﬁsh phage-DNA library made in kGEM-11(Promega, ... in plasmamembrane of Chinese hamster ovary and HEK293 cellsbut the S acMC5R was retained in intracellular compart-ments of these cells hindering pharmacological characteri-zation. The anatomical ... presence of SacMC3R in thebrain is not surprising, as the mammalian MC3R is foundprimarily in the brain. The mammalian MC3R has alsobeen found in some peripheral tissues such as placenta and gut....

Báo cáo khoa học: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily pot

... the casein kinase 2 pro-tein. Phosphorylation assay on the S13 3A ⁄ S13 4A mutantform of recombinant, His-tagged Grx4 (see Supportinginformation) was performed in the same way, but with a lower ... VII and XIV of Saccharomyces cerevisiae and basic phenotypic analysis of the mutant strains. Yeast16, 255–265.39 Marcand S, Gilson E & Shore D (1997) A protein-counting mechanism for telomere ... S25 8A mutant with that of the wild-type strain and of other bud32 mutants (D16 1A, K5 2A) and deletionmutants (bud32D), and observed that cells were almostRole of Bud32p in a new signaling pathway in...

Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx

... PP2Cenzymes may also have additional domains, for exam-ple, Arabidopsis thaliana ABI1 in which the catalytic domain is fused to an EF-hand motif, and human STP(HsSTP), which has an additional 8 kDa a- helicaldomain ... homolog-ous residues to Asp118 and Asp191 in HsSTP(Asp146 and Asp239) and, of course, in SaSTP(Asp118 and Asp192). Our SaSTP crystal structure provides clear evidence that the ﬂap subdomain is a mobile ... implications of the M3 binding and ﬂap subdomain conformations to the catalytic mechanism are discussed below.The role of the third metal in catalysis In MtSTP [9], Ser160 takes part in binding...

Báo cáo khoa học: Phosphorylation of the arginine/serine dipeptide-rich motif of the severe acute respiratory syndrome coronavirus nucleocapsid protein modulates its multimerization, translation inhibitory activity and cellular localization pptx

... 2008)doi:10.1111/j.1742-4658.2008.06564.xCoronavirus nucleocapsid protein is abundant in infected cells and partici-pates in viral RNA replication and transcription. The central domain of the nucleocapsid protein contains several arginine ... reports have suggested that the SARS-CoV N protein can act as a substrate of variouskinases, such as cyclin-dependent kinases, glycogen synthase kinase, creatine kinase II and mitogen-acti-vated ... reprogrammed to adapt tostress-induced damage. Translationally stalled mRNAstogether with a number of translation initiation factors and RNA-binding proteins are deposited into SGs[40]. Formation of SGs...

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