Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... c-lyases from
the enteric protozoan parasite Entamoeba histolytica
against physiological substrates and trifluoromethionine,
a promising lead compound against amoebiasis
Dan Sato
1,
*, Wataru Yamagata
2
, ... catalyzes the a, c-elimination
and c-replacement of l-Met and homocysteine (Hcy),
and a, b-elimination and b-replacement of l-Cys an...
... m
M Mes (pH 7) and substrate (5¢-G
2
CCCUCUAAAAA-3¢)at50°C
[6].
c
Substrate-cleavage reaction (exogenous guanosine-mediated)
of the substrate (5¢-CUUAAAAA-3¢) using the Anabaena ribozyme
(L-8 ... 1)
[14,17,21].
Dependence of substrate cleavage on pH
It has been reported that the rate of the substrate-
cleavage step in Tetrahymena [22–25], Anabaena [14]
and Azoarcus [17] grou...
... properties and subcellular localization patterns of the enzyme.
USP7 is phosphorylated at S18 and S963, and ubiquitinated at K869 in
mammalian cells. In in vitro activity assays, N- and C-terminal truncations
affected ... M1 and
E20, an alpha helix from there and up to amino acid
G28, followed by a b-sheet starting at T36 and extend-
ing to residue L49, and a larger...
... transla-
tion. The target application of the method is auto-
matic or semi-automatic compilation of a glossary or
technical-term dictionary for a certain domain. Re-
cursive application of the ... Automatic Collection of Related Terms from the Web
Satoshi Sato and Yasuhiro Sasaki
Graduate School of Informatics
Kyoto University
Sakyo, Kyoto, 606-8501
Japan
sato@i.kyot...
... observe the appearance of two
peaks at 1618 and 1683 cm
)1
, which are typical of
the formation of an intermolecular antiparallel b-sheet
aggregate [21].
The thermal stability of b-glucosidase was assessed
by ... acid and the DNA level, respec-
tively; they also have a similar catalytic mechanism
and substrate specificity. However, the molecular basis
of the high...
... LB400 through a PCR with GAGCGG
CATATGGA
AATCAAACCGAAGGTTCGCGA and GAGCGG
CATA
TGGAAATCAAACCGAAGGTTCGCGA as the forward
and reverse oligonucleotide primers, respectively. The
primers were designed ... Wellenreuther
and W. Meyer-Klaucke for data collection and assis-
tance in data evaluation. The assistance of T. Pavkov
(Institute of Chemistry, University of Graz) in the...
... 7.0 at 25 °C.
The effect of each ligand on related pairs of substrates
(neutral substrates o-NAC and o-NPA, and positively
charged substrates ATMA and ASCh) was compared. To
avoid complications ... analysis of BuChE AAA activity was carried out with
a positively charged acetanilide (ATMA), a negatively
charged acetanilide (NATAc) and neutral acetanilides
(o-NAC and...
... at the
same time, distinct absorption bands of oxyheme
appeared at 540 and 579 nm. Then, a broad band
appeared at around 660 nm, and was maximal 9–12 min
after initiation of the reaction. The ... estimated directly from the
values of absorbance at these maxima (data not
shown). The estimated association constants for imi-
dazole and azide binding to heme–GmHO-1 are s...
... DASA
polar
are the changes in
ASA of the apolar and polar residues, respectively
[39]. The structure-based calculations provide a DC
p
of
)407 calÆmol
)1
ÆK
)1
and DASA
apolar
and DASA
polar
of
)1354 and ... and without
the receptor N-domain using the vadar program
[41].
The DC
p
was calculated using the following equa-
tion:
DC
p
¼ 0:45DASA
apolar
À 0:26 ASA
p...