Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot

... contained a truncated core OS andconsisted of a linker (Glc-Kdo2)-lipid A structure. The phenotype of this mutant was examined to investigate the roles of different moieties of the LOS molecule ... those of the wild-type, indicating a truncatedLOS molecule resulting from the mutant (Fig. 3A).Western blot analysis demonstrated that the LOS from O35Elgt3 lost the binding activity to the mAb ... epithelial cells in vitro and in vivo, while show-ing hypersensitivity to hydrophobic compounds, indi-cating that LOS contributes to most biological activities of the M. catarrhalis outer membrane...

Báo cáo khoa học: A steady-state modeling approach to validate an in vivo mechanism of the GAL regulatory network in Saccharomyces cerevisiae ppt

... Furthermore, the status of one binding site could in uence the binding of the regulatory protein to the other,representing cooperativity [5,6]. A regulatory protein maybe the product of the ... interaction leading togene expression. The status of the switch is determined by the state of the upstream regulatory element of the GALgenes in the nucleus. The regulatory protein may bind to the upstream ... selected from the candidatemodels that best describe the input–output experimentaldata. T he individual components of the selected modelprovide insights into the importance of their roles in the overall...

Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc

... France.ResultsEvidence of different steps in the GPI-bALP mediatedmineralization: effects of Ca2+concentrationTo assess the role of Ca2+ in initiating the mineralizationprocess, we modelled the biological ... identiﬁcation by infraredspectraTo identify the quality and the type of mineral formationobtained in the mineralization medium during the GPI-bALP-induced hydrolysis of different phosphate ... lacking GPI-bALP didnot produce any mineral material. In uence of other phosphate substrates in the mineralization processTo examine the effects of substrates catalysed by GPI-bALP in the production...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... mediator of lethal sepsis, triggering the production of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... USA). Binding of all of the anti-bodies was detected using an ECL detection system (iNtRONBiotechnology, Seoul, Korea) in accordance with the manu-facturer’s instructions. The intensity of the ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, releasing largeamounts...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... basal membrane proteins to migrate apically. Transcytosis of integrinswould therefore enable H. pylori with the intriguingpossibility of targeting the integrin b1receptor at api-cal membranes ... transcytosis of integrin molecules from the basal to the apical side of the cells, a process thathas been suggested for integrin b1[15]. Indeed, disrup-tion of host-cell polarity by another pathogen ... Thus, the role of the CagL RGD-motif andCagY topology for injection of CagA is not yetresolved. It is also unclear why the effector proteinCagA itself can bind to the extracellular domain of inte-grin...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... and of the kanamycin insertion mutant nirF strain(SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom leftpanel and of SBN13 (DnirF) containingpEG276-nirF in the ... activity of NirFInterestingly, like NirN, NirF shares sequence similar-ity with the C-terminal d1heme-containing domain of cytochrome cd1. Strikingly, the axial ligand of iron in d1heme in ... in loss of in vivoNirF activity. We demonstrate here that the product of the nirF gene is aperiplasmic protein and, hence, must be involved in a late stage of the cofac-tor biosynthesis. In...

Tài liệu Báo cáo khoa học: Role of ceramide kinase in peroxisome proliferatoractivated receptor beta-induced cell survival of mouse keratinocytes ppt

... role in skin woundhealing. After a skin injury, PPARb expression is rap-idly elevated in the epidermis at the wound edges; dele-tion of a single PPARb allele results in delayed woundhealing ... in skin barrier disruption(Fig. 1A). PPARb mRNA expression was determined in keratinocytes isolated from the epidermal layer of these skins, and was found to be signiﬁcantly increased in skin ... (Fig. 3Ab) manner, indicating that activa-tion of PPARb is involved in the gene transcription of CERK. Furthermore, an in vitro kinase assaydetermined that CerK activities in whole-cell lysateswere...

Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidiﬁcation and cathepsin D in cholera toxin cytotoxicity pdf

... amounts of protein from 3Y1-Ad12 cell lysates confirmed the absence of cathepsin D in nontransfected cells, and the presence of both the 31 kDa mature cathepsin D and the 45 kDa procathepsin D in cathepsin ... con-trol of endosomal acidity was confirmed using cellular systems in whichmodification of the expression levels of cathepsin D, either by transfection of the cathepsin D gene or small interfering ... bafilomycin and the cathepsin D inhibitor pepstatin Apartially inhibited, both in vivo and in vitro, the cAMP response to choleratoxin. This cathepsin D-dependent action of cholera toxin under the con-trol...

Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc

... hafter the start of treatment with EGF, indicating thatEGF regulated the level of the Bcl-XLprotein at the transcriptional level. Next we determined the effect of ADR on the expression of Bcl-XL(Fig. ... proteins, is inducedthrough MAP kinase activation, allowing transactiva-tion of genes containing AP1-binding elements. How-ever, it is not known whether EGF is capable of protecting cells from ... transcription, were increased in response toEGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinasekinase, inhibited the EGF-induced c-Fos and c-Jun expression, AP1 DNAbinding, Bcl-XLexpression,...

Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx

... concentration. The dissociation constants, Kdvalues, enthalpies of binding and stoichiometries weredetermined from the binding isotherm by ﬁtting a 1 : 1binding model to the data using the software ... mutantproteins. This was interpreted as a lack of fosfomycinbinding to these mutant proteins [12]. Binding of fosfomycinto either wild-type MurA or the K22 mutant proteins in the absence of UDPNAG is not ... form of the protein. The results obtained with the K22V/R120K doublemutant protein point toward a central role of arginine 120 in the conformational process occurring during catalysis.Therefore,...

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