Báo cáo khoa học: Molecular mechanisms of the phospho-dependent prolyl cis ⁄ trans isomerase Pin1 docx

... destabilizing the double bond character of the pThr-Pro bond [26].Further evidence is available for the catalytic mecha-nism of Pin1 resembling more closely that of the other prolyl cis ⁄ trans isomerases, ... molecular mechanisms of the enzyme action. Most importantly,we want to critically review the evidence that Pin1 would or would not act as a prolyl cis ⁄ trans isomerase. Molecular mechanisms of Pin1 ... downstream of the proline could lead the WWdomain to read the cis form in a reversed manner. The initial screen for Pin1 substrates, which identiﬁed it asa phospho-dependent prolyl cis ⁄ trans isomerase, indicated...

Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identiﬁes a calcium sensitive region required for basal activity doc

... tation of the x MG P promo ter constru ctsused for transient transfections o f A6 cells is shown to the left. T he nomenclature of the promoter deletions is based o n the transcription start of the xMGP ... the molecular organization of the first nonmammalian M GP gene and the functional analysis of its promoter. We identified a region within the first 70 bp of the xMGP promoter that mediates transcriptionalactivation ... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4,probably corresponds to a premature arrest of the reversetranscriptase due to the presence of an inverted...

Báo cáo khoa học: Molecular basis of the unusual catalytic preference for GDP/GTP in Entamoeba histolytica 3-phosphoglycerate kinase doc

... aminogroup at carbon 2 of the guanine ring may interact with the side chain of Glu309, whereas the carbonyl group atposition 6 of the guanine ring may interact with the hydroxyl group of the Tyr239 side ... structures of the EhPGKobtained using the modeller program (Fig. S1),it was found that the only difference in the aminoacids that bind the purine ring was the presence of Valinstead of Leu at ... from the Vm ⁄ [Vm ⁄ Km] ratio [21], then the speciﬁc nucleotide-binding constant (Kd) was deter-mined in the absence of 3-phosphoglycerate for eachnucleotide in the wild-type and the double-mutantenzymes...

Báo cáo khoa học: Molecular dynamics of the DNA-binding domain of the papillomavirus E2 transcriptional regulator uncover differential properties for DNA target accommodation pdf

... which the majority of the protein motion is defined [27,28]. The analysis is basedon the diagonalization of the covariance matrix builtfrom the atomic fluctuations after the removal of the ABFig. ... that defines the surface of the gap region[26].In the interior of the HPV-16 b-barrel, at the inter-face of each dimer, only one small cavity, present for ashort percentage of the simulation ... distribution of molecular flexi-bility. The mechanical properties that characterize the two proteins, together with the different structural andconformational features of the spacer regions in the DNA...

Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc

... in the spleen of these mice. For the determination of the number of PMM56 bacteria present in the spleen of these four mice, we thus chose avalue of 50 CFU per spleen. The average number of ... deciphering the complexbiosynthetic pathways of DIMs and PGLs. Theseefforts have led to the identiﬁcation of more than 20proteins required either for the formation or for the translocation of these ... a position of the fattyacyl residues. The signals that correspond to the resonance of the methine proton of the a carbon (d) and that of the carbon bearing the methyl group near the keto group...

Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot

... by the structure of EcR [20]. Therefore, the elucidation of EcR and USP(RXR) structures is important for understanding the molecular mechanism of the action of 20E.In this study, we report the ... regions. The C-regions of EcRs andUSPs are highly conserved. However, other regions,particularly the N-terminal parts of USP ⁄ RXR, vary. The alignments of amino acid sequences of the A ⁄ B andE ... amino acids. The amino acid sequence of the L. australasiae ecdysone receptor was similar to that of the ecdysonereceptor-A of the soft tick, Ornithodoros moubata (68%) and to that of the ecdysone...

Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identiﬁcation of RGD/KGE sequences as binding motifs for C2 domains pdf

... mm Na2HPO4 ⁄ 1.8 mmKH2PO4 ⁄ 137 mm NaCl ⁄ 2.7 mm KCl ⁄ 1mm CaCl2 ⁄ 2mmMgCl2,pH 7.3 (NaCl ⁄ Pi). The cells were resuspended in10 mm Tris ⁄ HCl (pH 8.0) ⁄ 150 mm NaCl ⁄ 1mm EDTAcontaining ... fur-ther the speciﬁcity of the interaction, native cardosin Bwas used in the binding assays. Despite the high simi-larity between the two pistil aspartic proteinases, nei-ther the RGD nor the ... at the tip of a loop protruding away from the core of the protein. This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and istherefore reminiscent of RGD...

Báo cáo khoa học: Molecular characterization of secretory proteins Rv3619c and Rv3620c fromMycobacterium tuberculosisH37Rv docx

... constant (Kd) of 4.8 · 10)7M. The thermalunfolding of the heterodimer was completely reversible, with a Tm of 48 °C. The comparative thermodynamics and thermal unfolding analysis of the Rv3619c–Rv3620c ... determination of thermodynamic parame-ters. The parameters used in ﬁtting were the stoichiom-etry of association (n), the binding constant (Kb), and the change in enthalpy (DHb). The values of theseparameters ... Normalized transi-tion curves for temperature-induced transition of the complex monitored in the far-UV CD region at 222 nm. The thermal unfolding (j) andthermal refolding (•) proﬁles of the complex...

Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx

... members of the VOCsuperfamily. Superimposing P. shermanii MCE on the human glyoxalase structure shows that the Co2+ion of the MCE is only 0.2 A˚from the position of the Zn2+ion in the glyoxalase ... involved the insertion of several fragments of the 5¢-region of mce-1 into the mul-tiple cloning site of the vector pPD95.77 provided by A.Fire (Carnegie Institute, Baltimore, MD, USA). The inser-ted ... CoA; the existence of a hydrolase that is onlyFig. 7. Expression and puriﬁcation of the MCE-1. SDS ⁄ PAGE wasused to analyse the expression and puriﬁcation of the epimer-ase. Lane 1, molecular...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... the skuphenotype. Because G376V was a novel mutation inIVD, the effect of the mutation was further investi-gated by molecular modelling. Together with the previ-ously reported traits of the ... 4452–4463 ª 2010 The Authors Journal compilation ª 2010 FEBS 4453 the a-hydrogen of the substrate, and the other isG374, which forms the binding pocket for the branched-chain acyl moiety of substrate ... combination of or and sku phenotypes. The lowerpart indicates the representative results of genomic DNA directsequencing of PCR products harbouring the SNP 1127G>T of BmIVD from each of the three...

Xem thêm