... S41, S55, andS66. Northern blot analysis for estimation of mRNA expression of the transgene revealed higher expression in S41 and S55 mice and lower expression in S66 mice inthe liver, white ... activation, weperformed quantitative RT -PCR to measure themRNA expression levels of adipogenic genes, includingseveral PPARc-target genes, the transcription of which is enhanced in adipogenesis ... differentiation of adipose cells [34,35]. However, the concentrations of 15d-PGJ2used for activation of PPARc in most stud-ies are much higher (2.5–100 lm) than those of conven-tional PGs (picomolar...