Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx

... FEBS 5541 Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid M. T. A. P. Kresnowati*, W. A. van Winden, W. M. van Gulik and J. J. HeijnenDepartment of Biotechnology, ... (2001) Geneticmanipulation of 6-phosphofructo-1-kinase and fructose2,6-bisphosphate levels affects the extent to which benzoic acid inhibits the growth of Saccharomyces cerevisiae. Microbiology ... quantitative energetic and metabolic aspects have rarely beendiscussed. This study discusses use of the stimulus response approach to quantitatively study the energetic and metabolic aspects of the transient adaptation...

Tài liệu Báo cáo khoa học: Molecular and cellular speciﬁcity of post-translational aminoacyl isomerization in the crustacean hyperglycaemic hormone family docx

... FEBSas standards. Figure 2B shows that the elution time of Hep-dW4 is identical to that of the VIH II fragment.By MALDI-TOF MS, the mass of this fragment wasfound to be 795.8 Da, similar to the ... 41 Fig. 1. RP-HPLC proﬁle of an acetic acid extract of 30 lobster sinusglands. Only the part of the chromatogram where CHHs and VIHsare eluted is shown. The nature of the ultraviolet absorbance ... presence of the d-Trp inVIH II (not shown).Characterization of cell types in relation to CHH and VIH isomers To study the distribution of CHHs and VIHs in theX organ cells, immunohistochemical...

Tài liệu Báo cáo khoa học: Structural and functional speciﬁcities of PDGF-C and PDGF-D, the novel members of the platelet-derived growth factors family docx

... model of thePDGF-C growth factor domain indicates the disulﬁdebridges in PDGF-C to consist of Cys250 and 294,Cys280 and 335, and Cys287 and 337, and the inter-monomeric bonds to consist of Cys274 ... loops 1, 2 and 3 (Fig. 3A), and due to the head -to- tail arrangement,loop 2 of one monomer will be close to loops 1 and 3 of the other monomer when the dimer is formed(Fig. 3B). Six of the conserved ... of PDGF-C and -D have to becleaved extacellularly to make the C-terminal growthfactor domains active [8,10,12]. The CUB domains arebelieved to prevent PDGF-C and -D binding to theirreceptors...

Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

... D esign and synthesis of a p otent c yclic ana-logue of the myelin basic p rotein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation forinduction of experimental ... the design of synthetic p eptides and nonpeptide mimetics that c an act as vaccines o r artiﬁcialregulators of the immune response. Linear and c yclicanalogues of several MBP epitopes have ... proximity to the side c hain of Arg78, forcing it into a perpendicular position relative to theFig. 1. Ensembles of 3D structures of the agonist (A) and antagonist (B) linear analogues of MBP(74–85)...

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

... concentration of 50 mgÆL)1[20,21]. The puriﬁcation procedure was similar to that of the wild-type enzyme, but 3 mMdithiothreitol wasadded to all buffers and solutions to avoid oxidation of theincorporated ... factor of 18.8% and an Rfree of 22.3%with over 90% of the residues in the most favored region of a Ramachandran plot (Table 2). The resulting model isdepicted in Fig. 2. The Ca backbones of ... side chains of Asp280 and Ser222 at the beginning and end of domain B as well as bythe main-chain oxygens of Tyr315 (domain A) and Thr270(domain B) and by two water molecules. Ser222 of the Ca-Isequence...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... 2011Stability and fibril formation properties of human and fish transthyretin, and of the Escherichia colitransthyretin-related proteinErik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... seem to be functional and to influence aging in C. elegans, and are referred to as TRPs [69]. TTR-1 to TTR-57 are,however, not related to the YRGS TRP family [19], which is why we prefer to refer ... ther-mostability of hTTR, sbTTR, and ecTRP.ResultsPartial acid denaturation generates fibrils of hTTR and sbTTRPartial acid denaturation combined with turbidity assaysis a method frequently used to induce...

Báo cáo khoa học: Substrate and inhibitor speciﬁcity of Mycobacterium avium dihydrofolate reductase pptx

... designed in order to conﬁrm the functional importance of D31 and L32as substrate-binding, cofactor-binding and inhibitor-binding sites.The objectives of this study were to: (a) utilize site-directed ... of aspartate 27 of dihydrofolate reductasefrom Escherichia coli in interconversion of active and inactive enzyme conformers and binding of NADPH.J Biol Chem 265, 5579–5584.19 Birdsall B, Andrews ... fromthe number of residues of the recombinant DHFR (200).The secondary structure of the proteins, including regular and distorted a-helix, regular and distorted b-sheet, turns, and unordered...

Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx

... from tongue to stomach, but not in small and large intes-tines. Adh3 shows low and ubiquitous expression in all tissues and Aldh1 is observed at low to moderate levels in esophagus, stomach and jejunum, ... related members of theADH family. In both mice and rats, Adh4 is transcribed in the epithelium of tongue, esophagus and stomach, whereas Adh1 was active from stomach to rectum in mice, and in duodenum, ... ubiquitously and at low levels in all tissues.Low to moderate Aldh1 gene activity is observed in the epithelial cells of the tongue and esophagus, in the gastric glands and at the base of the...

Báo cáo khoa học: "Sentence and Expression Level Annotation of Opinions in User-Generated Discourse" potx

... theprofessors directly to me and two directed to my team.(3) I found that I spent most of my time learning from myfellow students.(4) A standard response from my professors would be that of a ... in-tensity), and to link it to the target and the holder.Figure 2 presents the expression level scheme.At this stage, annotators mark text spans, and areallowed to assign one of the ﬁve labels to themarked ... the annotators discussed witheach other if different decisions have been made and were allowed to ask questions to clarify theirunderstanding of the scheme. Annotators had ac-cess to the review...

Báo cáo khoa học: Natural and amyloid self-assembly of S100 proteins: structural basis of functional diversity doc

... Structural and functional insights into RAGEactivation by multimeric S100B. EMBO J 26, 3868–3878.12 Donato R (2007) RAGE: a single receptor for severalligands and different cellular responses: ... areimplicated and display marked changes in their expression levels in manytypes of cancer, neurodegenerative disorders, inﬂammatory and autoim-mune diseases. The structure and function of S100 proteins ... increases intoa cellular response. An increasing number of S100 pro-teins is also reported to occur extracellularly, binding to the receptor for advanced glycation endproducts(RAGE) [9–12] or Toll-like...

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