Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

... enriched in hydrophobicresidues. In yeast, proteins targeted to mitochondria Fig. 1. Overview of dual targeting of proteins to mitochondria and chloroplasts in plant cells. A gene encoding a dual- targeted ... mem-brane of chloroplasts (Toc)64 and Toc159 system usedby proteins involved in photosynthesis [36]. TheToc159 family in Arabidopsis consists of four proteins: Toc159, Toc132, Toc120 and Toc 90 ... present in mitochondria and chloroplasts (note that, for thepurpose of this minireview, dual targeted refers to proteins targeted to mitochondria and chloroplasts) ,the phenomenon of dual targeting...

Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf

... FEBSaccumulation of many proteins involved in photosyn-thesis (termed ‘photosynthetic proteins ), suggestingthat both atToc33 and atToc159 are involved in theimport of photosynthetic proteins. However, ... carrying a cytosolic preprotein. In the pathway, Hsp90 docks to the TPR repeats of Toc64 before the preprotein is handed over to Toc34[70]. Certain preproteins, such as the small subunit of Rubisco, ... is part of the Tob ⁄ Sam complex and is involved in the insertion of b-barrel proteins into the outermitochondrial membrane [28]. From an evolutionarypoint of view, it is likely that Toc75 has...

Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... domains aredepicted as columns. Regions involved in membrane binding are coloured in red, motifs involved in protein protein interaction are blue and the dehydrogenase domains of Tic32 and Tic62 ... four-transmembrane domain proteins. Topology-predictions indicate an N in /C in orientation. Tic62 belongs to the extended family of SDRs and can be divided in two distinct modules. The N-terminus contains the ... is in contrast to the function of these motifs in Hop and Hip[40,42,43]. Interestingly, binding of Tic40 to Tic110 isfavoured when the transit peptide-binding site of Tic110 is occupied by incoming...

Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx

... compilation ª 2009 FEBSMINIREVIEW Protein transport in organelles: Protein transport into and across the thylakoid membraneCassie Aldridge*, Peter Cain* and Colin RobinsonDepartment of Biological Sciences, ... conformation, allowing the transportation of proteins that fold too quickly or tightly for the Secpathway, or proteins that require the insertion of co-factors in the stroma before transport into the thy-lakoid ... during active protein transport, with the amphi-pathic helix and C-terminal tail interacting only in response to conditions leading to protein transport [30]. The Tat translocon needs to transport...

Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx

... min. Unboundpreproteins were washed off and bound preproteinseluted with 250 mm imidazole containing buffer. In every case, precursor protein was detected in the imid-azole eluate, indicating ... binding and hydrolysis [4–7].Toc34GDPinteracts with the second G -protein in theToc complex, Toc159, and simultaneously transfers thepreprotein to Toc159. Toc159 action facilitates pre- protein ... character-istics of the intermembrane space proteins Tic22 and MGD1, in vitro import experiments into isolated chloroplasts were performed using35S-labelled precur-sor proteins. Both pTic22 and pMGD1...

Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf

... hydrophobic TM segments of membrane proteins to exit laterally into the lipid phase. In bacteria, thetranslocation of secretory and membrane proteins occurs through a homologous channel in the plasmamembrane, ... biosynthesis of many proteins [1]. These includesoluble proteins, such as those ultimately secreted fromthe cell or localized to the ER lumen, and membrane proteins, such as those in the plasma ... the signal sequenceis intercalated into the walls of the channel and thesegment distal to it is inserted into the pore proper(Figs 1–3). In a ﬁrst step, the binding of a channelpartner (i.e....

Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf

... SENP2, protein inhibitor of activated STAT (PIAS) and Ran binding protein 2 (RanBP2) [16–20]. The N-terminal loops of SUMO proteins are not homologous to those of ubiquitin and Nedd8, and their ... migration inhibiting factor 2; Nedd8, neural cell-expresseddevelopmentally down-regulation protein 8; Pc2, polycomb protein 2; PIAS, protein inhibitor of activated STAT; RanBP2, Ran binding protein ... reaction.AbbreviationsAPPBP1, APP binding protein 1; CtBP, C-terminus binding protein; CTD, C-terminal domain; E1, activating enzyme; E2, conjugating enzyme;E3, ligase; HECT, homologous to E6AP C-terminus; IR, internal...

Báo cáo khoa học: Protein transport into canine pancreatic microsomes A quantitative approach potx

... analysis of protein transport into pancreatic microsomes in singleturnover translocation experiments, and of various othercomponents, believed to b e subunits of protein translocase and involved in ... quantity of protein, present in the respective band of a gel of a certain protein preparation, was determined by comparison with protein standards that were run on the same gel and stainedsimultaneously ... Speciﬁc binding of nas cent presecretory p roteins to microsomes in a single turnover experiment prevents cotranslational transport of other presecretory proteins i n a s ub sequen t transport...

Báo cáo khoa học: Protein glycation in Saccharomyces cerevisiae Argpyrimidine formation and methylglyoxal catabolism ppt

... and a high rate of methylglyoxal formation,hinting that glycation might occur in these cells [21]. Protein glycation by methylglyoxal in yeast, monitoredby argpyrimidine formation in proteins, ... growth in 9 days. At this time, cytosolic proteins were extracted and analysed by western blotting.Argpyrimidine-modiﬁed proteins were observed in all strains except BY4741 (Fig. 2B). Compared to ... phase of growth. A time course of argpy-rimidine formation in yeast proteins was then per-formed (Fig. 3A). Accumulation of the sameargpyrimidine-modiﬁed proteins, starting after only3 days of...

Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression proﬁling ppt

... through protein protein interactions. AaHR38 and AaSvp,during the arrest and termination of vitellogenesis,respectively, bind to AaUSP preventing its hetero-dimerization with AaEcR, and, consequently, ... presence of 20E,however, favors the formation of the AaEcR⁄ AaUSPheterodimer [13], indicating that the regulation of theactivity of these proteins occurs through protein pro-tein interactions, ... through protein protein binding with AaUSP [13], thus preventing formation of the functional ecdysone receptor and consequentlyinhibiting AaVg expression. AaHR38 sequestersAaUSP during the...

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