... sixadditional 5 min washings in PBG, and a further six 5 minwashings in NaCl ⁄ Pi, incubation with 2.5% (w ⁄ v) glutaral-dehyde in NaCl ⁄ Pi, and further brief washes in destilledwater, ... Caveolin-1-knockoutmice, indicating that Caveolin-1 expression was required for full expressionof P2X7 protein. Indeed, suppression of Caveolin-1 protein expression in E10 cells using short hairpin RNAs resulted in ... that, in immunohistochemical studies, plasma membrane stain-ing reflects the presence of the 80 kDa band alone.Isolation of lipid rafts and localizationof Caveolin-1 and P2X7Insolubility in the...