Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf
Báo cáo khoa học: Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi-rational design pdf
... http://www.pymol.org.Stabilization of pyranose oxidase O. Spadiut et al.792 FEBS Journal 276 (2009) 776–792 ª 2008 The Authors Journal compilation ª 2008 FEBS Improving thermostability and catalytic activity of pyranose 2-oxidase ... FEBS Improving thermostability and catalytic activity of pyranose 2-oxidase from Trametes multicolor by rational and semi -rational design Oliver Spadiut1, Christian Leitner1, Clara Salaheddin1, ... variant of pyranose 2-oxidase from thewhite-rot fungus Peniophora sp. Biochemistry 45, 6587–6595.7 Leitner C, Volc J & Haltrich D (2001) Purification and characterization of pyranose oxidase from...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... N-terminal and b-he licaldomains participate in binding of the polysaccharide,pullulan.Comparison of the active sites of the model of IPU and Dex49AThe active site structures of t he model of IPU ... 372, and 373are the potential catalytic r esidues of I PU. Also, Trp425 of Dex49A, which is the corresponding residue of Trp402 of IPU, is reportedly located in the vicinity of the active site and ... using TLC. T he W402F enzymeliberated isopanose from pullulan, and isomaltose and glucose from panose, and the action patterns of the wild-type and the W402F enzymes were almost identical (Fig....
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Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc
... residues of PEX1-ND are indica-ted by closed circles and closed squares, respectively (see text). The conserved residues and class-specific residues of PEX1-ND and VCP-ND are colored yellow and green, ... binding of wild-type and R144A mutant of theN-terminal domain (ND) of valosine-containing protein (VCP)-ND. (A)Two mutants of the conserved basic residues, R135A and K174A,were analyzed by PIP ... are AAA-ATPases. Dysfunction of the ATPase activity of either of the two proteins results in peroxisome biogenesisdisorders [4–7]. The ATPase activities of PEX1 and PEX6 are believed to be indispensable...
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Báo cáo khoa học: "Search in the Lost Sense of “Query”: Question Formulation in Web Search Queries and its Temporal Change" pptx
... accounted for by Q-queries. Weplot this against b’s activity level, measured by thenumber of queries issued by b in a month. We binnedusers by their activity levels on the log2-scale and compute ... analysis of query logs, e.g., Beitzel et al. (2004) and on longqueries, e.g., Bendersky and Croft (2009).Using co-clicking to infer query-query relation-ships was proposed by Baeza-Yates and Tiberi(2007). ... information and are defined by their syntactic behavior. As a result, most functionwords are treated as stopwords in IR systems, and web users often exclude them from queries. A highfraction of function...
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Báo cáo khoa học: Multidrug efflux pumps: The structures of prokaryotic ATP-binding cassette transporter efflux pumps and implications for our understanding of eukaryotic P-glycoproteins and homologues ppt
... analyses of cross-linking data and ligand docking, chiefly because of the NBD–TMDreverse domain order, the lack of conserved structuralmotifs ICD2 and ICD3, and shorter TMD helices and low sequence ... Signature sequence [21]. Also of note, TMhelices 4 and 5 are splayed away from one of theTMDs and form the majority of their interhelicalcontacts with TM helices from the opposite protomer.This ... pumps and implications for our understanding of eukaryoticP-glycoproteins and homologuesIan D. Kerr1, Peter M. Jones2 and Anthony M. George21 School of Biomedical Sciences, University of...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
... were performed by incubating 2 lLofin vitro35S-labeled product with 1 lg of Aos1 ⁄ Uba2, 150 ng of UBC9 and 1 lg of SUMO-1 insumoylation buffer (20 mm Hepes, pH 7.5, 5 mm MgCl2 and 2 mm ATP). ... withLRH-1 and to affect LRH-1-mediated gene activation.In addition, the activity of LRH-1 is modulated by post-translational modification. The transcriptional activity of LRH-1 is stimulated by phosphorylationfollowing ... [42,43]. By contrast, a lowconcentration of hydrogen peroxide induces proteindesumoylation by inhibition of conjugating enzymes activity through the formation of a disulfide bridge inE1 and E2...
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Báo cáo khoa học: B1–Proteases as Molecular Targets of Drug Development B1-001 DPP-IV structure and inhibitor design ppt
... theproduct was analyzed by SDS-PAGE and activity assay, show-ing a high reduction of the molecular mass and only a loss of the activity of the 20%. CD and 15N-1H-HSQC spectra of thisprotein fragment ... of a particular class of chymotrypsins within the gut of Abstracts166creates a better understanding of the specificity and selectivity of this enzyme and allows for further exploration and design ... accompanied or not by allosteric changes(d) by co-localization of enzyme and substrate(e) by cofactor binding accompanied or not by allosteric changes(f) by controlled substrate processing and access...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
... consists of the typical signals and spin systems of the indole moiety (triplets of 5-H and 6-H at7.12 and 7.18 p.p.m., doublets of 4-H and 7-H at 7.43 and 7.75 p.p.m and the singlet of 2-H at ... studies of E. cloacae IDPC (Eur. J. Biochem. 270) 2325indolepyruvate (triplets of 5-H and 6-H at 7.04 and 7.12 p.p.m., doublets of 4-H and 7-H at 7.39 and 7.42 p.p.m and the singlet of 2-H at ... pyruvatedecarboxylation.Examination of the decarboxylation of indolepyruvate by ScPDC and ZmPDCThe ability of ScPDC and ZmPDC to decarboxylateindolepyruvate was tested. In the case of ZmPDC no cata-lytic activity was...
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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... wereeluted from the gel matrix and analyzed by MALDI-TOF ⁄TOF MS.The PDI–bacitracin was characterized by a combination of in-source decay and CID-TOF ⁄ TOF MS, based on themethod described by Kleffmann ... separation of bacitracin by RP-HPLC, with a gradient of 10–90% ACN over 40 min and detection at 252 nm. Bacitracin A and F and bacitracin B and H were purified aspooled fractions of B 1–3 and H 1–3. ... open thiol form of bacitracin and Cys314 and Cys345 of PDI.PDI comprises four domains, a, b, b¢ and a¢, and anx-linker region between the b¢-domain and a¢-domain.The a-domains are catalytically...
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Báo cáo khoa học: Ca2+ ⁄ H+ antiporter-like activity of human recombinant Bax inhibitor-1 reconstituted into liposomes pdf
... The amounts of Ca2+released by acidic or alkaline pH were expressed as a per-centage of the maximum releasable Ca2+using the fluorescence and the radioactivity of 45Ca2+, and were compared ... result of the decrease in pH wasassayed by measuring the radioactivity of tritium, as described inMaterials and methods (In the figure, the y-axis represents thecpm values of pellet). Black and ... afunction of time. Lines a, b, c and d indicate an exterior pH of 6.5,6.0, 5.5 and 5.0, respectively. Line e represents the emission fluo-rescence in the absence of entrapped Ca2+. (B) The amount of...
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