Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

... dimerizationFEBS Journal 276 (2009) 760–775 ª 2008 The Authors Journal compilation ª 2008 FEBS 775 Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on ... USA) and Sigma (Poznan´, Poland).Construction of fusion proteins The human dopamine D1 and D2 receptor genes werecloned into the pcDNA3.1(+) plasmid and used as the starting point to construct ... manipulations of the Arg-rich epitope induced alterations in the localization of the resulting receptor proteins, leading to the conclusionthat this epitope is responsible for the cellular localization...

Tài liệu Báo cáo khoa học: Fatty acid synthesis Role of active site histidines and lysine in Cys-His-His-type b-ketoacyl-acyl carrier protein synthases ppt

... crystal-lization conditions included an ammonium ion concen-tration of 1.9 m, which probably in uences the nature of the cation under crystallization conditions. The function of the ion is presumably ... results of the decarboxylation assays of the ‘bulky’ mutants all pointto the orientation of H333 and its hydrogen bonddonor properties as being the cornerstone of obtaining and ⁄ or retaining residual ... in the binding of ACP [2], probablyby donating hydrogen bonds to one of the carbonyloxygens in malonyl-ACP, thereby stabilizing chargerelocalization during catalysis [3]. One reason for the low...

Tài liệu Báo cáo khoa học: Studies on structural and functional divergence among seven WhiB proteins of Mycobacterium tuberculosis H37Rv pdf

... monomer (Table 1). The sub-stoichiometric ironcontent of iron–sulfur proteins is generally due to the impaired incorporation of the cluster into the protein during overexpression in E. coli and ... in geneexpression after the deletion of whiB7 [6] could be seenas the absence of a component which possibly exertsits function via a protein protein interaction ratherthan a DNA protein interaction. ... [17], redox sensing [18] and the coordination of metal cofactors [19]. The functional importance of the conserved cysteine residues in iron–sulfur cluster coor-dination and protein disulﬁde reductase...

Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by ﬂuorescence resonance energy transfer and the role of ATP hydrolysis pptx

... function of OsDmc1 protein concentration (Fig. 4A, lines 2 and 3). How-ever, at concentrations higher than 2.5 lm OsDmc1,there was only an increase in the rate, but no furtherincrease in the ... function of reac-tion time, at a ﬁxed concentration of protein, by varyingnucleotide cofactor conditions. The reaction was the fastest when it contained ATP, where within about100–150 s the ... some criticalsteps in the conversion of inactive forms of protein tothat of active forms. It is interesting and intriguing tonote that in the absence of any nucleotide cofactor,OsDmc1 leads...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcation of the deubiquitinating enzyme otubain 1 modulates active RhoA levels and susceptibility to Yersinia invasion pptx

... wasfound to be increased upon overexpression of the deubiquitinating enzymeotubain 1 (OTUB1), a member of the ovarian tumour domain-containing protein family. Conversely, OTUB1 knockdown interfered ... the intestine. The Yersinia surface protein invasin binds to b1 integrins on the apical surface of M cells, which facilitates translocation across the epithelium [4,5]. The pathogenicity and virulence ... polyubiquitin chains[14,29,33], and is involved in anergy induction in CD4+lymphocytes through its interaction with the E3ligase gene related to anergy induction in lymphocytes(GRAIL) and ubiquitin-speciﬁc...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcations of the linker histone variants and their association with cell mechanisms docx

... in Swiss-Prot and similar databases.Structure and function of the linkerhistone variantsLocation and structure of the linker histonesHistorically, the location of the globular domain of the linker ... modiﬁcations at Lys26, and at posi-tion 27 there is an alanine, rather than a serine [43].Ubiquitination and formylationFor the ﬁrst time, ubiquitinations of the histone lin-ker protein were ... acetylation (lysine), phosphoryla-tion (serine, threonine), methylation (lysine, arginine),sumoylation (lysine), and ubiquitination (lysine). Otherepigenetic mechanisms may emerge in the future.Small...

Tài liệu Báo cáo khoa học: Suppressed catalytic efﬁciency of plasmin in the presence of long-chain fatty acids Identiﬁcation of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt

... content of the thrombi [4].These lipid constituents of thrombi profoundly modu-late the ﬁbrinolytic process [2,3,5–7]. In the few studies evaluating the effect of long-chain fatty acids on plasmin ... by plasmin (EC 3.4.21.7.), containup to millimolar concentrations of fatty acids and these are known toaffect the action of the protease. In the present study the modulation of plasmin activity ... models of plasmin inhibitionBecause, in most cases, the change in product concen-tration is not linear in the amidolytic assay performedwith the required concentrations of plasmin and sub-strate,...

Tài liệu Báo cáo khoa học: A novel splice variant of occludin deleted in exon 9 and its role in cell apoptosis and invasion docx

... important roles in the induction of mitochondria-mediated apoptosis and the inhibition of invasion, along with the downregulation of matrix metalloproteinase expression. In addition, byusing the calcium ... from the ER has been described as one of the mechanisms of this regulation. On the basis of the above ﬁndings, our research provides new insights into the role of exon 9 in the regulation of apoptosis ... cells on the bottom of the Matrigel-coated inserts. The number of invasive cells wasdetermined by counting the number of stained cells and then normalizing it to the number of control (Cont)...

Tài liệu Báo cáo khoa học: Speciﬁc membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc

... PE-containingmembranes, but rather for anionic membranes contain-ing PG [16], and little information is available regard-ing the phospholipid and membrane binding of the other members of the ... todate, and include the association with phospholipids and membranes [13–16], the inhibition of Raf1 kinase[17,18], thrombin [19], and G -protein- coupled receptor kinase 2 [20], and the N-terminal ... structure of the complex of ICwith CPY (IC–CPY) [8,9] have provided information on the nature of the inhibition. The N-terminal acetylgroup of ICis essential for inhibitory function, and the inhibitor...

Tài liệu Báo cáo khóa học: Non-speciﬁc depolymerization of chitosan by pronase and characterization of the resultant products pptx

... titrationcurve having two in exion points, whose difference along the abscissa corresponded to the amount of acid requiredfor the protonation of the amine groups of samples. The number of equivalents ... min (4 h) after which there was a decline (Fig. 4C,D),indicating enzyme stability in the pH range 2.0–5.0 and 45–60 °C in the initial hours. The effect of varying chitosanconcentration on the ... chitosan from the offal of marine food processing industry (and pronase), and show the biofunctionalities of these materials.AcknowledgementsAuthors thank Dr S. Subramanian (Indian Institute of Science,Bangalore)...

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