Báo cáo khoa học: Increased susceptibility of b-glucosidase from the hyperthermophile Pyrococcus furiosus to thermal inactivation at higher pressures pptx
... Increased susceptibility of b-glucosidase from the
hyperthermophile Pyrococcus furiosus to thermal
inactivation at higher pressures
Marieke E. Bruins
1
, Filip ... be a cause of
inactivation at higher pressures. No dissociation into
subunits was observed. One similar example from the
literature showed that inactivation of the dimeric
almond b-...
... Whereas the amounts of total and phosphory-
lated c-Fos increased in the presence of ATZ + mer-
captosuccinic acid, there was no change in the levels of
total and phosphorylated c-Jun throughout the ... LMW-PTP [13].
The cysteines in the active sites of these phosphatases
are easily inactivated by ROS, resulting in activation
of the ERK pathway [14]. However, factors...
... conformational states of the monomeric form of
the enolase. A schematic representation of the dissoci-
ation and association of r-Pfen is presented in
Scheme 1, where M
nt
is the native monomer, ... representation of dissociation of the dimeric
(D) form of enolase to the imidazole-induced and pH-induced mono-
meric (M
im
or M
pH
) forms. The dimer is shown to d...
... than
hepatocytes from 11–13-week-old male rats.
Expression of glycogen-targeting proteins in
hepatocytes from fa ⁄ fa rats
To test whether the defect in hepatocytes from fa ⁄ fa
rats is associated ... phosphorylase-a
and glycogen synthesis in hepatocytes from fa⁄ fa rats by modulating the
concentration of phosphorylase-a. Treatment of hepatocytes from fa ⁄ fa
rats and Fa...
... details of these signaling events have yet to be
investigated, many of the molecular interactions down-
stream of the Eph and ephrin molecules appear to lead to the
regulation of the cytoskeleton of ... recently,
the Src family kinases (SFKs) and the PTP-BL phosphatase
were proposed to be mediators of the phosphorylation state
of the cytoplasmic domain o...
... function of the instability of the
70 kDa subunit. Obviously, the D subunit is a substrate of
the I subunit, which led to questions concerning the
assembly of the I and D building blocks into the ... studies of deuteroporphyrin IX
to the H-subunit show a K
d
value of 0.53–1.2 l
M
[5]. The
large subunit has therefore been suggested to be the catalytic
subu...
... glycolysis, the
TCA cycle, the oxidative limb of the pentose phos-
phate pathway (OPPP), the urea cycle. However, these
investigations do not provide data that can be used to
calculate the fraction of the ... Also
these data by themselves do not allow us to calculate
the fraction of the flux that is channeled. The first
additional requirement is to show that the...
... unwise to eliminate them at this
point in the research. What is indicated, perhaps, is the
structural classification of the weak suffixes by degree
of weakness as a means of approaching the suffix-in- ... all the affixes could not be ex-
pected to show up in two-vowel-string words. This paper summarizes the
results of applying a modified form of the operation...
... FEBS
reach saturation for HSA concentrations well lower
than in the absence of myristate. This indicates that
binding of myristate to the modulatory site also increa-
ses the affinity of Mn(III)heme ... coordinated
to the metal ion or bound to the protein in close proximity
of the paramagnetic center the dipolar interaction is modu-
lated by the reorientation of...
... BP800, Helsinki, Finland). The growth rate was cal-
culated as the ratio of the absorbance of the experimental
well to that of a blank well, and the IC50 was also calculated.
Hoechst stain
Cells ... medulloblastoma cells, perhaps because of the
methylation of the promoter, and restoration of DKK1
expression can induce apoptosis and suppress colony
formation [42]. A...