Báo cáo khoa học: Caenorhabditis elegans metallothionein isoform speciﬁcity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 potx

... Authors Journal compilation ª 2009 FEBS Caenorhabditis elegans metallothionein isoform specificity – metal binding abilities and the role of histidine in CeMT1 and CeMT2 Roger Bofill1,*, Rubeń Orihuela1,*, ... Zn(II) CeMT1 and Cd(II) CeMT1 and 5 : 1 for Zn(II )– and Cd(II) CeMT2, Zn(II )– and Cd(II)–CtCeMT1 and Zn(II )– and Cd(II )– CtCeMT2. Additionally, Zn(II)–DHisCeMT2 and Cd(II )– NtCeMT1 were also incubated ... noteworthy in view of their coordinative potential, CeMT1 includesfour histidines, whereas CeMT2 only has a terminalone. In the absence of a comprehensive analysis of the metal- binding abilities of CeMT1...

Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modiﬁed gold electrode pot

... roles in the catalytic process including modulating the rate of electrontransfer between MMOR and MMOH [4,5], altering the redox potential of the binuclear iron site [6], optimizing the interaction ... protein B). The active site of the enzyme is located on the a subunit of MMOH and consists of a binuclear iron species located in ahydrophobic pocket approximately 12 A˚beneath the protein surface ... the scan rate)that the MMOH protein was absorbed onto the electrode.This was conﬁrmed after repeated cycling for 40 min(Fig. 2). The signal remained unchanged after rinsing the electrode and...

Báo cáo khoa học: Caenorhabditis elegans expresses a functional ArsA pptx

... range of concentrations using 15 lgofpurified ASNA-1 protein in the presence of 1 mM ATP. The reactionwas initiated by the addition of 5 mM MgCl2after 10 min incubation of the ASNA-1 protein ... completed within 30 min of recovering the protein preparation.Affinity of ASNA-1 protein for substratesTo examine the affinity of the ASNA-1 protein forATP, the apparent Kmfor ATP was determined atpH ... reaction was initiated by the addition of MgCl2after 10 minincubation of ASNA-1 protein at 37 °C. Solid and dashed lines indi-cate fitting of data to the Michaelis–Menten equation by a nonlinearregression...

Báo cáo khoa học: Caenorhabditis elegans has two genes encoding functional D-aspartate oxidases pot

... cloning the cDNAs of these C. elegans genes and expressing the recombinant proteins in E. coli. The enzymatic and kinetic properties of the proteins were then determined.This is the first time the ... and animal tissues [2 8–3 0]. In addition, the DAAO-enco-ding genes of yeasts [3 1–3 3], fungus [34], fishes [35], and mammals [3 6–4 2], and the DASPO-encoding genes of yeast [43] and mammals [4 4–4 6] ... use.Detection of recombinant proteins The protein concentrations in the crude extracts, insolublefractions, and purified enzymes were determined by the method of Bradford [72] using BSA as a standard....

Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx

... that the most characteristic signatures in the sequences are present in both proteins, tyrosinases and catechol oxidases; these include the six histidine resi-dues involved in the binding of a ... betweenmonophenols and o-diphenols as favored substrate, and also related to the presence or absence of a seventh histidine, adjacent to the sixth histidine involved in copper binding. Preceding the sixth histidine (H3Baccording ... signatures of tyrosinases, including the CuA and CuB binding sites to ligand the copperTable 3. Kinetic parameters for the two PPOs. The enzymes wereobtained from extracts of R. solanacearum strains...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... mechanism of the deamination reaction and the subsequent metabolism, including the deaminationstep. The product formed from 4-amino-3-hydroxybenzoicacid by the action of purified 4-amino-3-hydroxybenzoate2,3-dioxygenase ... of the purified enzyme at 266 nm. The typicalprotein absorption p eak of 2 80 nm shifts to 265 nm if the protein contains a flavin-type cofac tor [28]. We failed toidentify the cofactor of the ... enzyme The activity of the enzyme present in the crude extract of strain 10d that used 2 -amino-5-carboxymuconic 6-semi-aldehyde as substrate was measured by monitoring the increase in the absorbance...

Tài liệu Báo cáo khoa học: "Mining User Reviews: from Speciﬁcation to Summarization Xinfan Meng Key Laboratory of Computational Linguistics " doc

... but canbe inferred from the text. Qi Su et al. (2008) in- vestigates the problem of extracting those kinds of features. There approach utilizes the associa-tion between features and opinion words ... eachkind of product. Table 1 shows that our clusteringmethod is effective in collecting product features. The number of features increases rapidly with the number of speciﬁcations input into ... run the al-gorithms described in Hu and Liu (2004a) on the same data as the baseline.From Table 2, we can see the precision of base-line system is much lower than its recall. Examin-ing the...

Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc

... hour of binding, regardless of the input titer (Fig. 1B).FPGN-containing peptides facilitate the uptake of adenovirus into CD40-expressing cellsTo test the ability of CD40 -binding peptides in ... obtained in the absence of peptide, indicating that the FPGN motif is essential. To eliminate the possibilitythat nonsequence speciﬁc amino acid interactions could becontributing to CD40 binding, ... detachedfrom the virus in an in vivo setting – particularly because of the generally low (micromolar) binding afﬁnity of shortpeptides for their ligands. This may explain why previousstudies using bifunctional...

Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx

... phospha-tidylinositol 3-kinase (PI3K), Akt and glycogensynthase kinase 3 (GSK3). Together, our resultssupport a role of P2X7 receptors in the regulation of neuritogenesis and in the ﬁne control of the ... lm in the presence of Mg2+ and 68.23 ± 5.714 lm in its absence. No differences werefound in the maximal effect or in the Hill coefﬁcients, the latter being 2.625 ± 0.596 in the presence of Mg2+ and ... support the involvement of P2X7 receptors in the maintenance of neuroblastoma cells in a nondifferentiated state, and point to P2X7 receptor antagonists as future potentialtherapeutic agents in the...

Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s ﬂuorescent probe for the determination of glutathione doc

... proceed for 10 min, and the ﬂuorescence was then measured at room tem-perature. In uence of interference The in uence of the main constituents of plasma on the determination of 2.00 · 10)6molÆL)1GSH ... solution and showed high binding ability because of the two adjacent bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelaterings to coordinate ... effect on the ﬂuorescence intensity, 2.00 mL of buffer was chosen in subsequent experiments. In uence of the concentration of metallobridgedbis(b-CD)s 2 The in uence of the concentration of 2 on...

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