Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
Báo cáo khoa học: Unusual stability of human neuroglobin at low pH – molecular mechanisms and biological significance docx
... conditions of acidic pH, is essentially a random coil polypeptide.Urea-mediated denaturation studies revealed that the stability displayed byapoNgb at pH 2.0 is very similar to that of Mb at pH 7.0. ... maximum at 287 nm and the minimum at 283 nm and that between the maximum at 287 nm and the mini-mum at 295 nm were used to calculate the Tyr exposure. Unusual acid stability of human neuroglobin ... region of Mb at pH 8.0 and pH 2.0.1.01.20.60.80.20.4holoNgb, pH 7.0apoNgb, pH 2.0holoMb, pH7 .0Urea (M)02468100.0[θ]/[θ]0Fig. 5. Urea-mediated denaturation of Ngb and Mb. The denatur-ation...
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Tài liệu Báo cáo khoa học: High activity of human butyrylcholinesterase at low pH in the presence of excess butyrylthiocholine pptx
... panel). At low pH, bkcatis larger thankcat, but at high pH bkcatis smaller than kcat.Thatistosay,A328W goes from substrate activation, at low pH, tosubstrate inhibition, at high pH. A ... bkcat and kcat at high pH is large relative to the difference at low pH, and the difference between bkcat at high pH and bkcat at low pH is relatively large (Fig. 5). In all of these enzymes, there ... the molecular basis of substrate activation in wild-type and mutants of human BuChE [11,15,16], as well as substrate activation inwild-type and mutants of human and Bungarus fasciatusAChE at low...
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Báo cáo khoa học: Unusual fluorescence of W168 in Plasmodium falciparum triosephosphate isomerase, probed by single-tryptophan mutants docx
... Unfolding of triosephosphate isomerase from Trypanosomabrucei: identification of intermediates and insight into thedenaturation pathway using tryptophan mutants. Arch.Biochem. Biophys. 399, 11 7–1 29.51. ... withammonium sulfate at a saturation of 7 0–9 0% followed byanion-exchange chromatography using a ResourceQ(6 mL; Pharmacia) column at pH 8.0 (20 mMTris/HCl)with a linear gradient of 0–0 .5MNaCl. ... isomerase.Triosephosphate isomerase (TIM) is an important glycolyticenzyme that catalyses the isomerization of dihydroxyace-tone phosphate and glyceraldehyde 3-phosphate. Like mostTIMs, the...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... enzymes, membranes and molecules tocope with the heat. At low temperatures, the chemicalreaction rates are lower, and hence, in order to becompetitive and grow fast at low temperatures, evolu-tionary ... substrate, and it is possiblethat the enzymes also have adapted strategies toremove Na+around the phosphates of DNA beforecatalysis can take place. It seems clear that the salt-adapted and ... thatVsEndA adapts to lower temperatures by increasingthe kcat. The similar Kmvalues of the two enzymesmay indicate that VsEndA is meant to function at highsubstrate concentrations, at...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
... plasmid for GST and GST-fused proteins (GST–IST1, GST–IST1MIM, GST–IST1MIML326D,L353A, GST–CHMP6NT, GST–pHis and GST–MIT–pHis). Expression of GST–IST1, GST–IST1MIM and GST–IST1L326D,L353Awas ... were incu-bated with glutathione–Sepharose beads immobilizingGST–pHis (negative control) or GST–MIT–pHis for 2 h at 4 °C with gentle mixing. After Sepharose beads had beenrecovered by low- speed ... inten-sities of the immunoreactive bands for FLAG–IST1 inthe pulldown products of GST–MIT–pHis were muchstronger than those of FLAG–CHMP1B and FLAG–CHMP4b. For FLAG–CHMP6 (negative control),...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... gel-filtrationFig. 2. Effect of orthophosphate and di- and tri-polyphosphates onoligomerization of native hTK1. hTK1 was diluted and incubatedwith 3 mM of the indicated nucleotides or phosphate ... absence of MgCl2, as the combination of MgCl2 and Chaps causes a heavy precipitate.Fig. 1. Effect of nucleotides and polyphos-phates on oligomerization of native hTK1.Approximately 10 ng native ... slow dissociation may indicate that hTK1 is ahysteretic enzyme.The finding that the two linked phosphate groupsin pyrophosphate are sufficient for formation of thetetramer clearly shows that...
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Tài liệu Báo cáo khoa học: Biochemical characterization of human 3-methylglutaconyl-CoA hydratase and its role in leucine metabolism docx
... Isomers of 3-MG-CoA as substrates for human 3-MG-CoA hydratase (AUH). (A) 3-MG-CoA was enzymatically synthesized by incuba-ting 100 mM 3-methylglutaconate in 100 mM potassium phosphate pH 7.0 ... cleavage of citrate on inactivated citratelyase. Eur J Biochem 37, 29 5–3 04.26 Loupatty FJ, Ruiter JPLIJ, Duran M & Wanders RJ(2004) Direct nonisotopic assay of 3-methylglutaconyl-CoA hydratase ... with(Z)-glutaconate [21]. The activity of Gct using theCoA-donor acetyl-CoA and the CoA-acceptor 3-meth-ylglutaconate was relatively low. Our data suggest thatGct produces three isomers. The production of (Z)-3-MG-5-CoA...
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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
... Eu3+ at 320 nm and monitoring the XL665emission at 665 nm.Analysis of dataData were analysed using the computer programGRAPHPADPRISM(GraphPad Software Inc.). [3H]Spiperone saturationbinding ... monoclonalanti-gp120 Ig; NIBSC) for 1 h at 4 °C on a rotating wheel.Then, 2 5–5 0 lg of protein G–sepharose (Sigma) was added and incubation was carried out at 4 °C overnight on arotating wheel. Samples were ... of 0. 5–2 .5 · 106cellsÆmL)1 and passaged every 2–3 days. For infections, cellswere seeded at a density of 0. 3–0 .6 · 106cellsÆmL)1 and infected when they reached log-phase growth, i.e. at...
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Báo cáo khoa học: Catalytic digestion of human tumor necrosis factor-a by antibody heavy chain pot
... of incubation (lane 2), the bands at 19 and 20 kDa had disappeared, and two clearbands appeared at 15.0 and 13.3 kDa. These bandsare thought to be fragments of hTNF-a .At2 0hofincubation (lane ... 8 h of incu-bation, the bands at 19 and 29 kDa had disappeared, and two clearbands appeared at 15.0 and 13.3 kDa. These bands are thought tobe fragments of hTNF-a. After 20 h of incubation, ... 2, 3, 4, and 5: 0, 8, 20, 50 and 94 h of incubation, respectively, after mixing of hTNF-a and ETNF-6-H. Lanes 6, 7, 8, 9, and 10: 0, 8, 20, 50 and 94 h of incubation, respectively, of hTNF-a...
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Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
... Chem 28, 1–2 .42 Kabra PM, Lee HK, Lubich WP & Marton LJ (1986)Solid-phase extraction and determination of dansylderivatives of unconjugated and acetylated polyaminesby reversed-phase liquid ... of immunofluorescent staining of SMO in cells overex-pressing each of the isoforms provided direct visuali-zation and confirmation of protein localizationwithout the possibility of contamination ... direct visualization of the localization of human spermine oxidase in a human tumor cell line.We also identify and classify SMO5 as a third human polyamine catabolic enzyme that possesses the...
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