Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... samples in vivo and in vitro. We also describe the use of NMR spectroscopy to determine the binding mode of new low molecular mass inhibitors of the West Nile virus NS2B–NS3 protease which werediscovered ... The Authors Journal compilation ª 2009 FEBS 4255 NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease Xun-Cheng Su1, Kiyoshi Ozawa1, Hiromasa ... et al. NMR analysis of the West Nile virus protease FEBS Journal 276 (2009) 4244–4255 ª 2009 The Authors Journal compilation ª 2009 FEBS 4253minants of the NS2B cofactor of the West Nile virus two-component...

Báo cáo khoa học: NMR study of cellulose and wheat straw degradation by Ruminococcus albus 20 pdf

... polysaccharides degraded and of the sugars solubilized should aid in the understand-ing of the action of the cellulolytic system and in the evaluation of its efficiency in the degradation process.We ... thoseobserved at the given times. The concentrations of the metabolites were calculated from the quantification of the signals in the 1H NMR spectra relative to those of the internal standard TSP-d4.TLCTLC ... of other metabolites could not be identifiedbecause of the low concentration and splitting of the signals by the 1J1H,13Ccoupling constant. However,they appeared in the HSQC spectrum.The...

Tài liệu Báo cáo khoa học: "A study of Information Retrieval weighting schemes for sentiment analysis" doc

... features produces the best results.A subsequent innovation was the detection and removal of the objective parts of documents and the application of a polarity classiﬁer on the rest(Pang and Lee, 2004). ... maxt(tf) is the maximum frequency of anyterm in the document and avg dl is the averagenumber of terms in all the documents. For ease of reference, we also include the BM25 tf scheme. The k1 and b ... classiﬁcationscenario by localizing the estimation of idf to the documents of one or the other class and subtract-ing the two values. Therefore, the weight of term1388Table 2: SMART notation...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... average co-ordinates (the blue colour denotes the side chains of Arg2, Tyr4, Ile5, His6 and the yellow the side chains of Val3 and Pro7) (a) with the the X-ray structure of AII in the Fab131–AII complex ... 2003On the molecular basis of the recognition of angiotensin II (AII) NMR structure of AII in solution compared with the X-ray structure of AII boundto the mAb Fab131Andreas G. Tzakos1, Alexandre ... twolimiting cases. The intermediate values (6.4 Hz) of the backbone couplings for Tyr4 and His6 are possibly the result of twisting or bending in the middle of the amino-acidsequence of the hormone...

Báo cáo khoa học: NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1) pot

... polymorphism of monomeric subunit c isdue to the interaction of the monomer with the artificialenvironment lacking the stabilizing protein/protein contactswithin the ring. The undecameric c ring of the ... table, and resist boiling in SDS for 5 min in the case of the undecameric c ring of P. modestum [41]. This stability of the ring makes structural flexibility rather unlikely, indicatingthat the ... ting the two in teractingresidues. I n the row Dd(13Ca) t he difference be tween the ob served shifts and the co rrespo nding random coil values [34] is sh own. The sequ encelocations of the...

Báo cáo khoa học: "Empirical Study of Predictive Powers of Simple Attachment Schemes for Post-modifier Prepositional Phrases" pptx

... sentences. In another study, Whittemore, Ferrara, and Brunner (1989) quantify the occurrence of PPs in the 13 dialogues in terms of the syntactic types to which they attach and the overall syntactic ... b. the size of the hotel The algorithm for the other group is: Check to see if preceding lowest consti- tuent is a definite NP and part of a partitive expression, If it is, attach the ... together. The reason for this particular grouping was that there were a num- ber of functions evident in some PPs that occurred very infrequently and since one of the major foci of the study...

Báo cáo khoa học: A study of microRNAs in silico and in vivo: diagnostic and therapeutic applications in cancer pot

... study of microRNAs in silico and in vivo: diagnostic and therapeutic applications in cancerScott A. Waldman1 and Andre Terzic21 Departments of Pharmacology and Experimental Therapeutics and ... leukemia cells [31]. Indeed,there is a reciprocal relationship between the expres-sion of miR-15a and miR-16-1 and of Bcl-2, and the heterologous production of these miRNAs suppressesBcl-2 ... outcomes and prediction of therapeutic responses [25]. Whilethere are numerous detailed reviews in this ﬁeld, the purpose of this minireview was to provide, in overview,a summary of the potential...

Báo cáo khoa học: A study of microRNAs in silico and in vivo: bioimaging of microRNA biogenesis and regulation doc

... and cloned between the cytomegalovirus (CMV) promoter and the start codon of the Gaussia luciferase gene of the optical reporter gene vector (Fig. 1C). The Bioimaging of miRNA biogenesis and regulation ... pre-miRNA by Dicer. The synthetic duplex form of pre-miRNAs carrying quencher and organic dye at each strand can be designed. In the presence of the Dicer enzyme, the quencher molecule and ﬂuorophore ... dis-placement of the ﬂuorescence dye from the quenchingmolecule by the Dicer enzyme, which cleaved the syn-thetic let-7 precursor miRNA (Fig. 1D) [19]. In the absence of the Dicer protein, the emission...

Báo cáo khoa học: Structural study of the catalytic domain of PKCf using infrared spectroscopy and two-dimensional infrared correlation spectroscopy pot

... esti-mation of the bandwidth and the intensity of the bandsmaking up the amide I or I¢ region. Thereafter, curve ﬁttingwas performed and the heights, widths and positions of each band were optimized ... this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the effect of thermal unfolding in the ... When the cat-f spectra obtained in the absence (Fig. 4A) and in the presence (Fig. 4B) of MgATP were compared the widening of the overallamide I¢ contour was less in the presence of MgATP and the...

Báo cáo khoa học: Kinetic study of the HIV)1 DNA 3¢-end processing Single-turnover property of integrase docx

... together,these results suggest that the DNA-binding step corres-ponding to phase I accounts for the formation of non-speciﬁc complexes and that, at the beginning of phase II, the majority of complexes ... product. The stability of IN during the time-course of the reaction and the inﬂuence of protein aggregation are discussed.ResultsGeneral features of the 3¢-end processing kinetics The kinetics of ... complexes were very sta-ble over the time-course of the reaction. Binding isotherms of IN to DNAsubstrate and product also indicate tight binding of IN to the reactionproduct. Therefore, the...

Xem thêm