Báo cáo khoa học: Modiﬁed PCR methods for 3¢ end ampliﬁcation from serial analysis of gene expression (SAGE) tags doc

... high-throughput; methods; mRNA; rapid RT -PCR analysis of unknown SAGE tags (RAST -PCR) ; reverseSAGE (rSAGE); serial analysis of gene expression (SAGE); tag; two-step analysis of unknown SAGE tags (TSAT -PCR) CorrespondenceZ. ... FEBSREVIEW ARTICLE Modified PCR methods for 3¢ end amplification from serial analysis of gene expression (SAGE) tags Wang-Jie Xu1, Zhao-Xia Wang1and Zhong-Dong Qiao1,21 College of Life Science and ... tag-specific primer; RAST -PCR, rapid RT -PCR analysis of unknown SAGE tags; rSAGE, reverse SAGE; SAGE, serial analysis of gene expression; TSAT -PCR, two-step analysis of unknownSAGE tags. FEBS Journal...

Tài liệu Báo cáo khoa học: "Modiﬁed Distortion Matrices for Phrase-Based Statistical Machine Translation" doc

... pair of chunks (cx,cy):mode A×A : create a shortcut from each word of cxto each word of cy;mode L×F : create only one shortcut from the lastword of cxto the first of cy.The former ... fault of the reordering mod-els, but rather of a too coarse definition of the searchspace. To have a concrete idea, consider that a smallchange of the DL from 5 to 6 words, in a sentence of 8, ... Meeting of the Association for Computational Linguistics, pages 478–487,Jeju, Republic of Korea, 8-14 July 2012.c2012 Association for Computational Linguistics Modified Distortion Matricesfor...

Báo cáo khóa học: Modiﬁed colorimetric assay for uricase activity and a screen for mutant Bacillus subtilis uricase genes following StEP mutagenesis pptx

... 37 °Cforamaximum of 18 h with shaking to induce the expression of mutant uricase. Then, they were treated at 60 °Cfor1hforcell lysis and release of the protein. Following the addition of substrate, ... reaction.The utility of this assay was demonstrated in a screen for mutant uricase enzymes derived from the uricase gene of thethermophilic bacterium Bacillus subtilis by a modiﬁed stag-gered ... oxidation of uric acid in the presence of oxygen,which oxidizes uric acid to allantoin and CO2, leavinghydrogen peroxide as the reduction product of O2. Severalforms of uricase from microorganisms...

Báo cáo khoa học: Modiﬁed merozoite surface protein-1 peptides with short alpha helical regions are associated with inducing protection against malaria docx

... signiﬁ-cant population of conformations in the a region of the /wspace.The presence of NOEs between P15 d protons and T14a proton for peptides 24148 and 24326, and V14 a proton for peptide 23754, ... used for the structural analysis (NMR and CD). CD spectra analysis of monomers andpolymers, at a concentration where these peptides weresoluble in water, showed similar structural conformation(data ... appropriatedilutions of monkey sera (starting at a dilution of 1 : 40) for antibody analysis. Reactivity was observed by ﬂuorescencemicroscopy using the F(ab¢)2fragments from a 1 : 100-diluted...

Tài liệu Báo cáo khoa học: An ecdysteroid-inducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori docx

... achieved.Determination of the structure of 8K-BLPMALDI-TOF MS analysis of the puriﬁed material from the ﬁnal HPLC yielded a monoisotopic mass of 7409.2 ([M + H]+) (Fig. 3A). Amino acid sequence analysis of the ... ILPs.Production of 8K-BLP by fat bodyTo identify the tissue that produces 8K-BLP, gene expression of 8K-BLP in various tissues was analyzedby real-time quantitative RT -PCR (qRT -PCR) . The8K-BLP gene was ... source of the hemolymph 8K-BLP.Induction of 8K-BLP gene expression andsecretion by ecdysteroidThe onset of 8K-BLP production shortly before orafter pupal ecdysis suggested the involvement of ABCFig....

Tài liệu Báo cáo khoa học: Amino acid discrimination by arginyl-tRNA synthetases as revealed by an examination of natural speciﬁcity variants doc

... transferase from yeastoriginated from the stocks of H. Sternbach (formerly Max-Planck-Institute, Go¨ttingen), while that from E. coli inrecombinant form was provided by A. Weiner (University of Washington ... & Cramer F (1978) Amino-acyl-tRNA synthetases from yeast. The generality of chemical proofreading in the prevention of misaminoa-cylation of tRNA. Biochemistry 17, 3459–3468.30 Rosenthal ... FW(1984) Cloning and expression of the gene for bacterio-phage T7 RNA polymerase. Proc Natl Acad Sci USA81, 2035–2039.59 Rosenthal GA (1977) Preparation and colorimetric analysis of l-canavanine....

Tài liệu Báo cáo khoa học: The RNA recognition motif, a plastic RNA-binding platform to regulate post-transcriptional gene expression ppt

... type of nucleotide either at the5¢ or the 3¢ position. The nucleotides at these two posi-tions always adopt an anti conformation, except for the G at the 3¢ position always found in a syn confor-mation. ... beingspread on the b-sheet from b4to b2in the 5¢ )3¢ direc-tion. More often, the nucleotide at the 5¢ end of thecentral dinucleotide contacts the loops at the bottom of the b-sheet (loop 1 and ... eukaryotes. Analysis of the structures of this domain in the freeform as well as in complex with both RNA and pro-teins shows that this small domain is extremelydiverse in terms of both structure...

Báo cáo khoa học: Gc recruitment system incorporating a novel signal ampliﬁcation circuit to screen transient protein-protein interactions pot

... (Gccyto) is genetically prepared and substituted for the endogenous Gc, resulting in inter-ruption of signal transduction owing to cytosolic translocation of the Gb subunit from the membrane. ... cells. The formation of diploid cells in mediumlacking methionine and lysine depends on the affinities of the protein pairs [12].To verify our hypothesis that the incorporation of asignal amplification ... highly sensitive amplification system probablytriggered the formation of background diploid cells,owing to the leaky expression of intact Gc in responseto the extremely low level of basal signaling....

Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

... [20]:koff¼ðkoffð2ÞÂð½ibuprofen=ðK2Â½ibuprofenÞþðkoffð3ÞÂð½ibuprofen=ðK3Â½ibuprofenÞÞ þ kþoffð7Þwhere koff(2)and koff(3)indicate values of koffoccurring atK2< [ibuprofen] ... for ibu-profen binding to tHSA–heme-Fe(II)-NO (K2and K3) wereobtained from the dependence of koffon the free ibuprofenconcentration. Values of K2and K3were determined from data analysis, ... 10)1s)1).Ibuprofen binding to tHSA–heme-Fe(II)-NOFigure 4 shows the binding isotherm for ibuprofenbinding to tHSA–heme-Fe(II)-NO. Analysis of thedependence of the molar fraction of the ibuprofen-bound...

Báo cáo khoa học: Ca2+/calmodulin-dependent kinase II signalling cascade mediates P2X7 receptor-dependent inhibition of neuritogenesis in neuroblastoma cells docx

... primer,5¢-AGGGCAGATGTCCAGAGCAT -3¢; reverse primer,5¢-GTCTTCATCAGCCCAGCAGTT -3¢. PCRs involved aninitial denaturation step at 94 °C for 5 min, followed by 40cycles of amplification (94 °C for 30 s, 60 °C for 30 s ... concomitant expression of GFP allowedtransfected cells to be identified by fluorescence microscopy.RT -PCR and quantitative real-time PCR Total RNA was extracted from cultured N2a cells and from whole ... 5317agonists or antagonists exist for all of the P2X recep-tors, and therefore their pharmacological identificationrelies on the effects of a number of compounds (for areview, see [69]). ATP and...

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