Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
Báo cáo khoa học: Functional analysis of mutations in UDP-galactose-4epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells pdf
... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... UDP-galactose-4-epimerase (GALE) associated with galactosemia in Korean patients using mammalian GALE-null cells You-Lim Bang1,*, Trang T. T. Nguyen1,*, Tram T. B. Trinh1,*, Yun J. Kim1, Junghan ... on the mutantproteins.We previously described nine GALE mutations in Korean patients with GALE-deficient galactosemia [20]. These GALE mutations were novel with theexception of R335H, which had...
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Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis pdf
... sub-strate acetyl-S-Ppant-PCP. Interestingly, after 5–10 min of incubation at 37 °C, no further hydrolysis of the remain-ing substrate was observed, indicating clear instability of this enzyme under the ... kDa), which is associated with theproduction of the secondary metabolite surfactin [12,24].A total of 11 TEIISrfmutants, including mutations of allstrictly conserved residues with functionalized ... the removal of short acylchains originating from aberrant decarboxylation of chainextender units from the thiol moiety of the 4¢-Ppantcofactors of acyl carrier proteins [15,16]. In NRPSs thereis...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... group of GHMP kinases in which proton transfer does not play amajor role in the rate determining step of catalysis.That five of the 10 disease-causing mutations resulted in insoluble protein in ... 2003homoserine kinase, proton transfer is unlikely to play amajor role in the rate-determining step of catalysis.Several of the disease-causing mutations are not soluble following induction in E. ... solvent kinetic isotope effect was measured by deter-mining the kinetic constants as described above in thepresence of increasing mole fractions of D2O(Aldrich).Kinetic constants of the mutantsThe...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... °Cor22 °C), by using an alternative expression host{BL21(DE3)[pLysS]} or by including protease inhibi-tors in the solutions used during purification.Kinetic analysis of disease-causing mutations The ... Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE). Crosslinking was carried out with N-(3-dimethylaminopropyl)-N¢-ethylcarbodiimide (EDC), as described in ... enabled the creation and analysis of all nine currently known disease-causing mutations in this protein. All but one of these mutantproteins (L183P) could also be produced in an activeand soluble...
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Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt
... N-terminal region of DEC1, including thebHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto ... region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identification of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 mutants with ... ncubated with a controlprotein.Fig. 6. Identification of t he domain of B MAL1 that inte racts with DEC1. Interactions of various truncated BMAL1 mutants with DEC1 in NIH3T3 cells were examined. pBIND,...
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Báo cáo khoa học: Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin-binding adhesins fibronectin-binding protein A and fibronectin-binding protein B from Staphylococcus aureus pot
... FEBS 4505 Functional analysis of a murine monoclonal antibodyagainst the repetitive region of the fibronectin-bindingadhesins fibronectin-binding protein A andfibronectin-binding protein B from ... fibronectin. TheC-terminal domain of fibronectin-binding protein A is organized into 11tandem repeats, six of which bind the ligand with high affinity; other sitesbind more weakly. Fibronectin-binding ... 2010)doi:10.1111/j.1742-4658.2010.07835.xFibronectin-binding proteins A and B are multifunctional LPXTG staphy-lococcal adhesins, comprising an N-terminal region that binds fibrinogenand elastin, and a C-terminal domain that interacts with...
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Báo cáo khoa học: Functional analysis of the aglycone-binding site of the maize b-glucosidase Zm-p60.1 pot
... aglycone-binding site of Zm-p60.1differs distinctly from that of Bgl4:1 and its homologs.These findings offer exciting prospects for comparative analysis of the molecular determinants of substrate ... modeling to obtaininsights into the molecular interactions underlying theobserved changes in the mutants’ enzymatic kinetics.Modeling was only applicable to F193A because inter-pretation of ... distances of reactingatoms. The only noted difference was in the orienta-tion of the aromatic ring of pNPGlc in the F193Amutant (Fig. 6D), owing to lost van der Waals contact with the side-chain of...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... (C1–3) loops. In addition, GPCRs contain a more or less extendedN-terminal domain and C-terminal domain, which areboth often involved either in ligand binding or G-pro-tein coupling.Owing to their ... 4B).Localization of the ET-1-binding site In order to determine the ETB region that is essentialfor binding of ET-1, a series of eight plasmids codingfor terminally truncated ETB fragments and contain-ing ... possible with digitonin [27]. Accordingly, specific detergentsABFig. 8. Analysis of dimerization of truncated ETB fragments with full-length ETBStrepcontaining a StrepII-tag. Interacting proteinseluted...
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Báo cáo khoa học: Functional analysis of two divalent metal-dependent regulatory genes dmdR1 and dmdR2 in Streptomyces coelicolor and proteome changes in deletion mutants ppt
... DNA-binding protein of the ferritin family563 nt20 052 DaContains domains typical of the ferritin-likesuperfamily, such as: DNA-binding ferritin-like protein, ferritins and bacterioferritinsPutative ... proteins in comparison with those of other actinomycetes revealed a strong conser-vation of motifs in domains 1 and 2 ( 70% identicalresidues), particularly in the DNA-binding region(domain ... aminoacid stretch that occurs in DmdR2 but is absent in DmdR1 and in the rest of the proteins of this family(domain 3, Fig. 2B).Disruption of dmdR1 alters significantlythe protein profile in...
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Báo cáo khoa học: Functional analysis of the methylmalonyl-CoA epimerase from Caenorhabditis elegans docx
... over 12 min at a flow rate of 1mLÆmin)1. Quantification was by integration of peak areas using breeze software (Waters, Milford, MA, USA). TheMCE activity was determined over a range of enzyme ... of the domains occurs in several differentways, suggesting that the evolution of these proteinsprobably involved multiple gene duplication, genefusion and domain swapping events. Members of ... The basic strategy involved the insertion of several fragments of the 5¢-region of mce-1 into the mul-tiple cloning site of the vector pPD95.77 provided by A.Fire (Carnegie Institute, Baltimore,...
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