Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... splicing is important for ES cell self-renewal and differentiation[3]. However, the mechanisms by which molecules thatKeywords cell cycle; embryonic stem cells; knockout mouse; polypyrimidine tract-binding ... viable,they form compact colonies and exhibit severe defectsin cell proliferation without precocious differentiation.Our data clearly demonstrate that PTB is essential for mouse development and ES cell ... causative gene in Okihiro syn-drome, is essential for embryonic stem cell proliferation, and cooperates with Sall1 in anorectal, heart, brain and kidney development. Development 133, 3005–3013.25...
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Báo cáo khoa học: Signal peptide hydrophobicity is critical for early stages in protein export by Bacillus subtilis ppt
... endogenousa-amylase of B. subtilis (AmyE), the halo assays were per-formed with amyE mutant B. subtilis 168 X strains.Subcellular localization of proteinsThe subcellular localization of proteins in E. coli ... for providing anti-Trigger Factorof E. coli, R. Brimacombe for providing anti-L23 ofE. coli, M. Marahiel for providing B. subtilis SG1, and P. Genevaux for providing E. coli secB. Funding for the ... Sciences and Biotechnology Institute, University of Groningen, the NetherlandsBacillus subtilis and Escherichia coli are used as proto-type models for studies on protein translocation and secretion...
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Báo cáo khoa học: The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus doc
... for disks.Results for PapMV disks and CP6–215 disks differedfrom those for F13L and F13Y disks, and suggest thatthere is still some RNA associated with recombinantF13L and F13Y disks. This ... three forms were as follows: NLP,33%; disks, 36%; and monomers, 31%. This currentprofile differs slightly from the first one that we pub-lished [9]. This is probably because the methods used for ... formation of nucleocapsid-likeparticles (NLPs) that are very similar to wild-typevirus purified from plants [9]. Therefore, this system is ideal for investigating virus assembly as well as for mapping...
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Báo cáo khoa học: The b domain is required for Vps4p oligomerization into a functionally active ATPase potx
... Vps4p, are essential for interaction with Vta1p. Inaddition, we show that one of these motifs is required for a homotypic interaction and for formation of ahighly active ATPase complex, but is not ... GAI motif is not essential for Vps4p recruitmentto endosomes.The Vps4p GAI motif is not essential for ATPase activityTo determine whether the GAI motif in the b domain is important for Vps4p ... AAA+ proteins is well established. Our study,however, shows that for Vps4p, regions outside of theAAA domain are also required for assembly. Althoughthis finding was unexpected, there is a...
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Báo cáo khoa học: The chromosomal protein HMGN2 mediates lipopolysaccharide-induced expression of b-defensins in A549 cells potx
... were treatedwith 0, 20, 40, 60, 80 or 100 lgÆmL)1LPS for 24 h and then the RNA and protein were isolated for RT-PCR and western blot analysis. The results demon-strated that LPS induced HBD-2 ... HMGN2 is crucial for LPS-inducedHBD-2 expression in A549 cells. The cellswere incubated in DMEM with 10% FBScontaining 0, 20, 40, 60, 80 or 100 lgÆmL)1LPS for 24 h and then HBD-2 mRNA and protein ... replaced 6 hafter transfection and the cells were cultured for 24 h beforethe efficiency of HMGN2 knockdown was determined.cDNA microarray and data analysisA549 cells were placed into six-well...
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Báo cáo khoa học: Sp3 transcription factor is crucial for transcriptional activation of the human NOX4 gene doc
... Sp3 is essential for postnatal survival and tooth development [18], hematopoiesis [19] and myo-cardial development [20]. The involvement of Sp1 and ⁄ or Sp3 in transcriptional activation is gene-spe-cific. ... in cell lysates was determined and normalized according to Renilla luciferase activity.Statistical analysisValues were expressed as the mean ± standard error. Sta-tistical analysis was performed ... nonessential amino acids and 10% fetal bovine serum.Reporter constructs and luciferase assayHuman genomic DNA was isolated from SH-SY5Y cellsusing a PUREGENE DNA Isolation Kit (Gentra Systems,Minneapolis,...
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Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx
... with increased risk of cardiovascular disease, the leading cause ofdeath. Therefore, it is important to understand the molecular basis underly-ing obesity and related metabolic diseases in order ... resistance [15]. Here, we focus onthe roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obes-ity and related metabolic diseases, and discuss whetherthese ANGPTLs could be targets for the prevention and ... inobesity and related metabolic diseases.The expression of ANGPTL2 and ANGPTL6 ⁄AGF is induced in obese conditions.ANGPTL2 induces chronic adipose tissueinflammation and systemic insulin resistancethrough...
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Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
... variable reduction and linear discrimi-nant analysis was performed on sensitive and multiresistantcells spectra in order to establish a diagnostic tool for multiresistant pattern. For each of t ... nd K 562/DNS cells is not significantin the amide protein. This is not surprising as spectra werenormalized for equal content of proteins. But a qualitativechange of the proteins, for example ... discriminant analysis (LDA).Thisstatisticalmultivariate method is supervised. It searches for thevariables containing the greatest interclass variance and the smallest intraclass variance, and...
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Báo cáo khoa học: "Machine Aided Error-Correction Environment for Korean Morphological Analysis and Part-of-Speech Tagging" pptx
... model (Brill, 1993). This tagging system is a hybrid system using both statistical training and rule-based training. Rule-based training is performed only on the statistical tagging errors. ... transformation rules dealing with agglutinative characteristics of Korean, and also extends the tagger by using specific transforma- tion rule considering the lexical information of mistagged ... model is defined first to suggest candidate tags to the user and then to find words which is likely to be wrong tagged. Correction rule 1016 and manual correction log are necessary for au-...
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Báo cáo khoa học: "Semi-Supervised Conditional Random Fields for Improved Sequence Segmentation and Labeling" pdf
... gene and protein mentions in bio-logical texts, and show that incorporatingunlabeled data improves the performanceof the supervised CRF in this case.1 IntroductionSemi-supervised learning is ... Computational Linguistics and 44th Annual Meeting of the ACL, pages 209–216,Sydney, July 2006.c2006 Association for Computational LinguisticsSemi-Supervised Conditional Random Fields for Improved ... models, and therefore it is preferable to focus on discriminativeapproaches. Unfortunately, it is far from obvioushow unlabeled training data can be naturally in-corporated into a discriminative...
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