... were treatedwith 0, 20, 40, 60, 80 or 100 lgÆmL)1LPS for 24 h and then the RNA and protein were isolated for RT-PCR and western blot analysis. The results demon-strated that LPS induced HBD-2 ... HMGN2 is crucial for LPS-inducedHBD-2 expression in A549 cells. The cellswere incubated in DMEM with 10% FBScontaining 0, 20, 40, 60, 80 or 100 lgÆmL)1LPS for 24 h and then HBD-2 mRNA and protein ... replaced 6 hafter transfection and the cells were cultured for 24 h beforethe efficiency of HMGN2 knockdown was determined.cDNA microarray and data analysisA549 cells were placed into six-well...