... with the exception of the N-terminus and the TM III–IV loop. To examine the functions of TjZNT1 and TjZNT2, these transporters were taggedwith hemagglutinin (HA) at their N-termini to verifytheir ... However, the approach lead to the identification of the extracellu-lar N-terminus as a substrate selector in TjZNT2. Onalignment of the amino acid sequences of reported ZIPtransporters, the length and ... werecloned into the pTAII vector (Toyobo Co., Ltd, Osaka,Japan), and subcloned into the pKT10-Gal-HA-BS yeastexpression vector at the EcoRI–SalI sites to eliminate HAtags. Without a stop codon,...