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Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

Báo cáo khoa học: Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms docx

... 2009 FEBS Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms Lorenzo Dolcini1, Alberto Sala1, ... variety of important physi-ological processes. Upon binding they regulate the availability of both insulin-like growth factors I and II(IGF-I and -II) and their affinity for these ligands ismodulated ... 2009)doi:10.1111/j.1742-4658.2009.07318.x Insulin-like growth factor binding protein-1 (IGFBP-1) is the majorsecreted protein of human decidual cells during gestation and, as a modula-tor of insulin-like growth factors or by...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf

Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf

... Xenopus oocytes.Furthermore, this phosphorylation was regulated bycytosolic injections of PKA activators and inhibitors,suggesting it to be the basis for the functional effects of cAMP injections ... vivotreatment of the oocytes with a PKA inhibitor and also in untreated oocytes. On the other hand, the in vitro phosphorylation signal was markedly dimin-ished when SpCAMPS, a PKA activator, was ... FEBSincreases in the order G1 C-T > G1 dC-T > G1362)410(Fig. 2A, B) and is inversely related to the molecularmass of the constructs, indicating the highest enrichment of phosphorylation sites in...
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Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx

Báo cáo khoa học: Identification of the heparin-binding domains of the interferon-induced protein kinase, PKR pptx

... positions of PKR mutants and the mutant name is shown at the bottom of the panels. (C) dsRNA -binding activity of the mutants. The dsRNA -binding activity of the point mutants was tested by their binding ... change, unmasking the ATP -binding site and leading to autophosphorylation of PKR. Activated PKR then phos-phorylates the a-subunit of the protein synthesis initiation factor 2 (eIF2a)thereby inducing ... are as indicated at the bottom of the panels and posi-tions of the proteins are indicated by arrows. Additional bandsobserved below the expected bands arise due to initiation of trans-lation...
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Báo cáo khoa học: Identification of the N-terminal region of TjZNT2, a Zrt⁄Irt-like protein family metal transporter, as a novel functional region involved in metal ion selectivity ppt

Báo cáo khoa học: Identification of the N-terminal region of TjZNT2, a Zrt⁄Irt-like protein family metal transporter, as a novel functional region involved in metal ion selectivity ppt

... with the exception of the N-terminus and the TM III–IV loop. To examine the functions of TjZNT1 and TjZNT2, these transporters were taggedwith hemagglutinin (HA) at their N-termini to verifytheir ... However, the approach lead to the identification of the extracellu-lar N-terminus as a substrate selector in TjZNT2. Onalignment of the amino acid sequences of reported ZIPtransporters, the length and ... werecloned into the pTAII vector (Toyobo Co., Ltd, Osaka,Japan), and subcloned into the pKT10-Gal-HA-BS yeastexpression vector at the EcoRI–SalI sites to eliminate HAtags. Without a stop codon,...
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Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt

Báo cáo khoa học: Identification of the N-termini of NADPH : protochlorophyllide oxidoreductase A and B from barley etioplasts (Hordeum vulgare L.) ppt

... containsa Pchlide binding site [22,26], and that binding of Pch-lide to pPORA is essential for import into the etioplaststroma. The transit peptide of PORA is the only exam-ple of a precursor ... according to our find-ings, amino acids T56–G60 of PORA are locatedexclusively in the mature part of the PORA protein and not in the transit peptide. The position of the motif in the N-terminal ... of Pchlide to the mature part of PORA, which is C-termi-nal of the processing site. It remains open whetherPchlide binding to the proposed motif in maturePORA is of importance for regulation of PORAimport...
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Báo cáo khoa học: Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor pptx

Báo cáo khoa học: Identification of the epitope of a monoclonal antibody that disrupts binding of human transferrin to the human transferrin receptor pptx

... restricted to a surfacepatch of hTF consisting of residues 365–385. Mutagenesis of the F11epitope of hTF to the sequence of either mouse or pig TF confirmed the identity of the epitope as immunoreactivity ... protein and hTFbind to the same or closely overlapping sites [14] addsfurther support for the role of the helical domain of the TFR in binding to TF since most of the residues thatbind to HFE ... epitope of the F11 antibody is in the C-lobe of hTF, specificallywithin residues 365–401 of the C1 domain. Further-more, binding of the F11 antibody to hTF inhibits binding to TFR (Fig. 1). The...
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Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

Báo cáo khóa học: Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme pptx

... as the standards and their elution volumes, Ve, were deter-mined. The standard curve was plotted with the logarithm of molecular mass against Ve/V0 of the standard protein.Peptidase and ... their translocationinto the cavity of the cylinder of the proteins themselves [14].In the past decade, although ATP-dependent proteases of the AAA+superfamily have been shown to exhibitchaperone-like ... transcription of Bt-lon The lon gene of E. coli and B. subtilis belongs to the heat-shock regulon, the transcription of which is increased onheat induction through the action of the heat-shock-specificsigma...
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Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx

... but slightly less and later in mantle. Oneinducible and two constitutive isoforms of HSP70 aresynthesized in the gills and mantle of C. gigas [26]. The expression level of the constitutive forms ... transcription cofactor thatinhibits activation of AP-1 transcription factors. QMprotein is implicated in the conversion of a broad vari-ety of extracellular signals generated by growth factors,tumour ... watertemperature in the area. Our study is the first to applyan overall genomic approach to the study of the response of C. gigas to prolonged heat stress. We usedanimals outside the season of reproductive...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt

Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt

... belonging to the GTP- binding elongation factor family, which promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of deacylated ... the cytoplasm, a considerable fraction of the asubunit can be found either in the cytoplasm or the nucleus[35,36]. The involvement of eEF1A in the regulation of nuclear processes includes the ... software analysis system (Bio-RadLaboratories). To test the nuclear enrichment, the presence of twonuclear transcription factors – the early growth responseprotein 1 (Egr1) and the TATA-binding...
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Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx

Báo cáo khoa học: Identification of multiple isoforms of the cAMP-dependent protein kinase catalytic subunit in the bivalve mollusc Mytilus galloprovincialis potx

... articlewas to investigate the possible existence of different isoforms of the PKA C-subunit in the sea musselM. galloprovincialis.ResultsSeparation of different isoforms of the C-subunitIn order to ... activity of the C-subunit. The transitory increase of cAMP levelsinside the cell, induced by an extracellular signal, and the binding of cyclic nucleotide to R-subunits cause the dissociation of C-subunits ... demonstrate the presence of different iso-forms of the PKA C-subunit in mussels, the proteinwas partially purified from the PAM and the mantletissues of the mollusc, and then subjected to cationexchange...
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