Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... backbone and the osmolytes leading to the preferential hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional ... proteins[3,4], but they also induce refolding of misfoldedproteins [5–8] and remove protein aggregation [9–12].Mechanisms of protein osmolyte interactions, the effect of osmolytes on protein stability, ... opposite to the effects thaturea has on protein function [16–19].Despite signiﬁcant advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and...

Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO pptx

... 3177binding of an N-hydroxyguanidine moiety in the iNOSheme pocket thus roughly involves the same bindingenergy as binding of the guanidine moiety, and the equilibrium constants are mainly ... NOHA[10], nothing is known about the thermodynamics and kinetics of the binding of guanidines and N-hydroxy-guanidines to iNOS. Removal of the a-amino ora-carboxylate moiety of l-Arg has important ... favour-able binding enthalpy for the formation of the complex between NOHA and the protein. The crystal structures of l-Arg or NOHA in NOS active sites actuallyshowed that the a-amino acid moiety of these...

Báo cáo khoa học: Correlation between functional and structural changes of reduced and oxidized trout hemoglobins I and IV at different pHs doc

... Lucigenin-ampliﬁed chemiluminescence of the xanthine/xanthine oxidase reaction in the presence of 40 lgÆmL)1 of oxy and met derivatives of both hemoglobins. The system contained 0.9 U of xanthine ... R.W.,Pennelly,R.R.,Fung,L.W.,Ho,C.,Giannini,I.,Porschke,D.&Winkler, H. (1978) Interactions between the quaternary structure of the globin and the spin state of the heme in ferric mixed spinderivatives of hemoglobin. Biochemistry ... containing0.9 UÆmL)1xanthine oxidase, 40 lgÆmL)1 of hemoprotein and 150 lmolÆL)1lucigenin in 1 mL of the chosen buffer. The reaction was started by the injection of xanthine at the ﬁnal...

Báo cáo y học: " Relationship between anal symptoms and anal findings"

... Sims’ position, as the buttocks fall to each side, and finger tips of both hands of the investigator are free for gentle eversion of the anal skin with the help of a good lighting [5,14]. A fundamental ... reliable for determining the causes of anal bleeding, anal itch, anal pain or anal burning. The sensitivity, specificity and predictive value of patients’ positioning in diag-nosis of BAD, concomitant ... dry anal skin at anal verge in- cluding thickened, leathery surrounding skin tags (stage 4 of pruritus ani, definition table 3). Table 4: Definition of concomitant anal findings (CAF)...

Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc

... timing of steps involved in dimer formation and a ppearance of thermostabilization byphosphate has been examined. The role of the cofactor5¢-phosphate group in the induction of stability and stabil-ization ... formation of an a po-oligomer induced by the b inding of the nucleotide sugar. The presence of maltopentaose (5 mM) resulted in a moderate 1.5-foldincrease in the half-life of apo-CcStP.Effects of ... from the b eginning of the r econstitution or was added at the e nd of the burst phase (5 min). The yield was the same in bothexperiments within t he experimental error. The r ecovery of activity...

Báo cáo khoa học: Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1 – a molecular link between recombination and chromatin assembly during meiosis pot

... diagram of the trunca-tion mutants of CcCac1L. (C) Interaction between CcCac1L and CcLim15 in a yeast two-hybrid assay. The inserts in the activation domain(AD) and DNA-binding domain (BK) ... exonu-clease activity and invades the homologous double-stranded region of the other allele. These steps of homology search and recombination are catalysedby two bacterial RecA homologues, Rad51 and Lim15/Dmc1. ... accumu-lating evidence regarding the role of CAF-1 in chroma-tin assembly following various DNA synthesis events,its involvement in chromatin assembly following mei-otic recombination is unknown. In...

Báo cáo khoa học: Distinguishing between calpain heterodimerization and homodimerization doc

... on the PEFdomains of both the large and small subunits [33,34]. In the presence of Ca2+, subdomains A and C of calpastatin tightly associate with PEF domain IV of the large subunit and domain ... determine which of the other PEF domain-containing calpains form heterodimers and which formhomodimers. His6-tagged PEF domains of calpains 1, 3, 9 and 13 werecoexpressed with the PEF domain of the ... between the two subunits of these intracellular cysteine proteases is through a pairing of the ﬁfthEF-hand of their C-terminal penta-EF-hand (PEF) domains. From model-ing studies and observation of...

Báo cáo khoa học: "Dependencies between Student State and Speech Recognition Problems in Spoken Tutoring Dialogues" ppt

... ITSPOKE: Well The product of the mass of a body and its acceleration remains constant for a given force. What is the relationship between a force act-ing on the box, the mass of the box, and its ... CERT – REJ interaction is explained only by the interaction between Certain and Rej and the in- teraction between Uncertain and Rej. 5 Results - dependencies In this section we present all significant ... name the forces acting on the packet. Please, specify their directions.” asks for both the names of the forces and their direction). If the student answer is correct and contains all pieces of...

Tài liệu Báo cáo khoa học: "A Multimodal Interface for Access to Content in the Home" pdf

... understanding of system capa-bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... and pen input, includ-ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of movies-on-demand and supporting ... completing the same two sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re-mote control and the other...

Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx

... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH2at the QPsite [27].Study of the reduction and oxidation of the cytochromehas ... four kineticphases in the reduction of the hemes by menaquinol.According to the quinone type used, the two hemes of the cytochrome are probably involved in the transfer of electrons from the ... advantage of notdiluting the reducing solutions, thus allowing the addition of smaller quantities for the same reducing effect. In any event, the results obtained are equivalent whatever the mode of reduction...

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