... °C).Subsequently, the second and the third nested PCR wereperformed with LdEcR-F2 and LdEcR-R2 primers(52 °C) and with LdEcR-F3 and LdEcR-R3 primers(46 °C), respectively. The first PCR with LdUSP-F1 and LdUSP-R1 ... [29–33], and were also reported for a dipteran, the yellow fevermosquitoe Aedes aegypti [34], and lepidopterans, the tobacco hornworm Manduca sexta [35–37] and the silkworm Bombyx mori [38,39]. On the ... integument (INT) and whole body(WB) at day 4 in the last larval instar, in the INT at day 0, 2, 4, 6 and 8 in the last larvalinstar, and in the adult male WB (#), femaleWB ($), testis TES and ovary...