Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

Báo cáo khoa học: Vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide attenuate the cigarette smoke extract-induced apoptotic death of rat alveolar L2 cells ppt

... aliquots at )20°C until used. On the day of the experiment, one aliquot of the stock solutionwas thawed and diluted in buffer to the appropriateconcentration.RT-PCR analysis of mRNAs encoding PACAP/VIP ... analysis of the saturation data [23]. The ability of VIP and PACAP27 toinhibit the speciﬁc binding of 125I-labeled VIP (0.03 nM)wasestimated from the IC50values (the molar concentrations of unlabeled ... nuclear compaction,chromatin condensation at the periphery of the nuclearenvelope, and fragmentation of nuclei, as determined byTUNEL staining (Fig. 4). Under control conditions, theseevents...

Báo cáo khoa học: Human-blind probes and primers for dengue virus identiﬁcation Exhaustive analysis of subsequences present in the human and 83 dengue genome sequences doc

... of the virus which is commonin that region, a re-emergence of an earlier version, acontinuation of an outbreak from the previous seasonor the introduction of a new strain as the result of Fig. ... 3. Distribution of the percentage of n-mers per genome thatare unique (i.e. not contained in any of the other dengue genomesconsidered).Fig. 4. Distribution of the percentage of human-blind ... encephalitis, and dengue fever. One ormore of the four serotypes of the dengue virus areendemic in many parts of the world, including all of south-east Asia, parts of Africa, and Southern and Central...

Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc

... analysis, and the determination of released ammonia. The coupled enzymeassay revealed the mechanism of the deamination reaction and the subsequent metabolism, including the deaminationstep. The product ... one enzymatic and one nonenzy-matic, see below) to 2-hydroxymuconic 6 -semialdehyde and an almost equimolar concentration of ammonia. The Fig. 2. Ab sorption spectra of the reaction products formed ... and 95%, respectively.Spectroscopic characterization of the puriﬁed enzyme The c oncentrated enzyme solution (fraction 6) was yellow i ncolor. The enzyme s olution showed one main absorptionpeak...

Báo cáo khoa học: A novel metallobridged bis(b-cyclodextrin)s ﬂuorescent probe for the determination of glutathione doc

... bis(b-CD)s.Because of the conformation of the linker of 1, the nitrogen atoms and amido bond formed two chelaterings to coordinate with copper (II) ions. This coordi-nation effect and the paramagnetism of ... metallobridgedbis(b-CD)s 2 The influence of the concentration of 2 on fluorescenceintensity is shown in Fig. 4. As can be seen, as the concentration of 2 increased, the fluorescence intensity of the system also ... tem-perature.Influence of interference The influence of the main constituents of plasma on the determination of 2.00 · 10)6molÆL)1GSH werestudied. The criterion for interference was fixed at a± 5.0% variation...

Báo cáo khoa học: Cofactor-independent oxygenation reactions catalyzed by soluble methane monooxygenase at the surface of a modiﬁed gold electrode pot

... modulating the rate of electrontransfer between MMOR and MMOH [4,5], altering the redox potential of the binuclear iron site [6], optimizing the interaction between MMOR and MMOH [7], controlling the ... investigate the effects of catalase, MMOB and MMOBtru and the substrate acetonitrile on electrontransfer to MMOH was also performed between 0 and )0.6 V, at the scan speed and protein and substrateconcentrations ... mVÆs)1. The arrow shows the eﬀect of increasingcatalase concentrations (0, 2.4, 4.8 and 7.2 lM, respectively). The eﬀect of ventilating the reaction at the highest catalase concentration wasalso...

Báo cáo khoa học: From functional genomics to systems biology Meeting report based on the presentations at the 3rd EMBL Biennial Symposium 2006 (Heidelberg, Germany) Sergii Ivakhno pot

... functional association wasfurther aided by integration of protein interactiondata with data on gene expression, localization, func-tion, evolutionary conservation, protein structure and binary interactions. ... [15] and yeast [16], consist of physical and functional inter-actions between TFs and their target genes represented on the graph [17]. The systematic mapping of TF–targetgene interactions has ... more than 70% of the promoters are bound by at least one of the top 10%most highly connected TFs. In addition, 82% of the promoters are bound by at least one of the other less-well-connected interactors,...

Báo cáo khoa học: Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information potx

... MINIREVIEW Protein–protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information Mitsuo Umetsu1,2, Takeshi ... or NMR structural analyses and the construction of artiﬁcial libraries allow con-structive selection and functionalization not only of antibody fragments, but also of small, nonantibody proteins ... candidate location in NCS for grafting with the CDR 3 loop of VHH. (B) Insertion of the CDR 1-containing loop of the heavy chain in Fv into the candidate location in TOP. Generation of binding proteins...

Báo cáo khoa học: Krit 1 interactions with microtubules and membranes are regulated by Rap1 and integrin cytoplasmic domain associated protein-1 doc

... from the perinuclear region to the leading edge upon chemo-kine stimulation and activated Rap1 triggers the association of RAPL with aLb2, leading to the redis-tribution of this integrin to the ... and, for the second part, with the highercontribution to the binding to MT of the basic stretchthan the C-terminal site.At the cell periphery, Rap1 shuttles between recy-cling endosomes and ... nuclearlocalization sequence [23], suggesting that it could beinvolved in the shuttling of Krit1 between the micro-tubules and the nucleus. Remarkably, the contribution of these two sites to the binding...

Báo cáo khoa học: Coiled–coil interactions modulate multimerization, mitochondrial binding and kinase activity of myotonic dystrophy protein kinase splice isoforms pptx

... of multimerization promoted by the N terminus and the coiled-coil domain [16,17]. However, the consequences of multimerization and association with other proteins for in vivo activity regulation of DMPK ... properties of any particular aminoacid segment therein [24]. We compared the localiza-tion of YFP-tagged isoforms A, C and E, containing the L477G, L487G and L515G mutations, with that of the wild ... binding of the myotonic dystrophy protein kinase to the myosin phosphatase targeting subunit was strongly reduced. Further-more, the association of myotonic dystrophy protein kinase isoform C tothe...

Tài liệu Báo cáo khoa học: SREBPs: protein interaction and SREBPs Ryuichiro Sato doc

... increase in the number of proteins modiﬁedby SUMO, it has become obvious that the effects of SUMO conjugation are diverse and largely depend on the function of the protein targeted for sumoylation.Sumoylation ... recruitment of PGC-1a. Under fasting condi-tions, HNF-4 and PGC-1a vigorously activate the expression of gluconeogenic genes. In contrast, underfeeding conditions, SREBP-1c, the expression of whichis ... transcriptionfactor to direct the transcriptional activation of the chaperone molecules and enzymes essential for proteinfolding [22,23]. Overexpression of the cleaved form of ATF6, and also...

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