Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf
Báo cáo khoa học: Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster pdf
... processes involve expression of some Hsps in Drosophila [40,57]. Finally, it has been suggested that expression of Hsps during recovery from cold mightresult from the thermal stress experienced during ... studied. In the present study, we focused on 11 Dro-sophila melanogaster Hsp genes during the stress exposure and recovery phases. The temporal gene expression of adults was analyzed during 9 h of cold ... Colinet et al. Heat shock response to cold stress FEBS Journal 277 (2010) 174–185 ª 2009 The Authors Journal compilation ª 2009 FEBS 185 Temporal expression of heat shock genes during cold stress...
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Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt
... different from that of many active promoters,which are marked by binding of TATA-binding pro-tein and recruitment of protein phosphatase 2A[67,68]. In mouse lens, HSF4 binds to nonclassical heat shock ... constitutively in cells and binds to and regulates the expression of genes without anyapparent stress intervention [1,3].HSF2 and HSF4 may not be directly involved in the stress response, but rather in ... Spt6 and Spt16 are involved in histone eviction and redeposition, and inactivation of Spt16 leads to sustained transcription [61]. In Drosophila Studies in Drosophila provide many insights into...
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Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
... regulation and binding of heat shock factor 1 and heat shock factor 2 to 32 human heat shock genes during thermal stress and differentia-tion. Cell Stress Chaperones 9, 21–28.44 Ostling P, Bjo¨rk ... aid the folding of damaged proteins, and increased accumulation of HSPs is essential for sur-vival of cells exposed to protein-damaging stresses,including heat shock. The structure of HSF comprisesa ... protein) of YYY49 and YYT42 con-tained approximately 1 and 2 ng of hHSF2, and those of YYT50 and YYT17 contained approximately 0.01 and 0.1 ng of hHSF4, asjudged by the intensity of each band....
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Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt
... suggesting thatthe heterologous expression of genes encoding metallo-proteins can lead to incorrect metal ion incorporation.This is observed in rubredoxin and desulforedoxinswhere zinc, instead ... transformants.Protein techniquesProtein was determined by protein-dye binding with BSA asa standard [47]. Whole cell protein of C. ammoniagenes cellswas isolated from 2 mL of induced culture. ... that RNR of C. ammoniagenes con-tained an iron metallocofacor. In their latter study, theyused an R2F preparation originating from heterologous expression of the C. ammoniagenes nrdF gene in E....
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... cloning and cold- inducible gene expression of ferritinH subunit isoforms in rainbow trout cells. J Biol Chem271, 26908–26913.13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & ChuangMJ (1999) Cold- induced ... properties of the tetrameric enzymemay result in fine-tuning of the properties of skeletal LDH-A, which is in linewith previously observed kinetic and thermodynamic differences between cold and ‘warm’ ... of heat denaturation (scanningrate ¼ 2 °CÆmin)1between 10 and 110 °C)There are three independent transition states during denaturation: tetramer fimonomer fi domain 1 fi domain 2Dynamics of...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... ferritin expression in nonpermeabilized (thin lines,– saponin) and permeabilized cells (thick lines, + saponin) cells cul-tured without and with iron as indicated. (D) Perls’ staining of cyto-spins ... in iron-rich mediashowed a further increase in ROS production as deter-mined by an increase in DCFH mean fluorescenceintensity (Fig. 2B, thick line). In addition, determin-ation of acrolein ... 2004 FEBS 161Determination of phosphatidylserine expression and cell deathPhosphatidylserine expression on the outer part of theplasma membrane was examined by Annexin V binding.Briefly, cells...
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Tài liệu Báo cáo khoa học: Enhanced expression of Mcm proteins in cancer cells derived from uterine cervix docx
... Bluestaining.Table 2. Quantitation and comparison of proteins in GM and WI-38cells. From the data in Fig. 2 and others, the concentrations of Mcmproteins, ORC2 and PCNA in total protein and chromatin-bound ... products are involved in the higher level of Mcm expression in HeLa cells and cancer cells from humanuterine cervix.Although it remains to be determined how enhanced expression of Mcm proteins affects ... immunohistochemical techniques, and the results were compared with the findings obtained byhematoxylin and eosin staining. We examined the expres-sion of these proteins in a cancer-free layer of squamousepithelial...
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Báo cáo khoa học: Increased expression of c-Fos by extracellular signal-regulated kinase activation under sustained oxidative stress elicits BimEL upregulation and hepatocyte apoptosis pot
... aninhibitor of c-Jun N-terminal kinase, showed no effecton the DNA binding of c-Fos and c-Jun induced bytreatment with ATZ + mercaptosuccinic acid, indicat-ing that JNK is not involved in ... directly, leading to transactiva-tion of AP-1. On the basis of these findings, we nextexamined the expression and phosphorylation of c-Fos and c-Jun. The total amount of nuclear c-Fosincreased ... protein-1-binding site in the bim promoterregion. BimEL expression and hepatocyte apoptosis were suppressed byknockdown of c-Fos and c-Jun, respectively. These results indicate thatincreases in...
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Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... function of increasing amount of totalproteins in membrane fractions from I7 yeast strain induced at15 °C, and from yeast expressing SSTR2 as a control.J. Minic et al. Expression of olfactory ... results in overexpression of GAL4, in turn inducingan increase of the expression of the OR gene under control of GAL1. The pJH2 vector contains the URA3-selectablemarker.Two Gaprotein expression ... for increasing theyield of properly folded recombinant human gammainterferon from inclusion bodies. J Biotechnol 52, 127–133. Expression of olfactory receptors in yeast for screening J. Minic...
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Báo cáo khoa học: Differential effects of histone deacetylase inhibitors on phorbol ester- and TGF-b1 induced murine tissue inhibitor of metalloproteinases-1 gene expression docx
... acetylation of Ets1 are also unknown, but of future interest, as mutation of the Ets binding motif(m4) results in both a loss of protein binding and theTSA superinduction of Timp-1.Eleven NAD-independent ... the expression of a protein(s) required for induction of the Timp-1 gene.Time course of TSA action upon PMA- and TGF-b1-induced Timp-1 expression The time course of induction of Timp-1 gene expression by ... FEBSpatterns of expression both during development and in response to stimuli. Timp-1 gene expression can beinduced by a variety of stimuli including phorbol esters(PMA), serum, transforming growth...
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