... Japan) in low-salt buffer(20 mMHepes, 20 mMNaCl, 20% glycerol, 1 mMEDTA,1mM2-mercaptoethanol, pH 7.9, containing 1 lgÆmL )1 aprotinin, 1 lgÆmL )1 pepstatin and 1 lgÆmL )1 leupeptin)with3H-labeled ... (PerkinElmer LifeSciences, Wellesley, MA, USA) using a liquid scintillationcounter LSC-1000 (Aloka Co., Ltd, Tokyo, Japan).Ligand binding to the cell-free preparation fromC. suppressalisintegument Rice ... superfamily, whichis generally composed of six distinct regions (A–F), and thepredicted amino acid sequences of C (DNA binding) and E(ligand binding) regions are conserved among insects [11].Little...