Peptidylarginine deiminase (PAD) is a mouse cortical granule protein that plays a role in preimplantation embryonic development docx

... pepti-dylarginine deiminase) . Mouse cortical granules contain PADTo ascertain if mouse cortical granules contain PAD, anti-bodies made against mouse ePAD and human recom-binant PAD V (anti-PAD V ... Biology and EndocrinologyOpen AccessResearch Peptidylarginine deiminase (PAD) is a mouse cortical granule protein that plays a role in preimplantation embryonic development Min Liu1, Andrea ... retarded preimplantation development, suggesting that cortical granule PAD plays a role after its release in preimplantation cleavage and early embryonic development. Conclusion: Our data showed that...

Báo cáo khoa học: The Drosophila jumonji gene encodes a JmjC-containing nuclear protein that is required for metamorphosis pot

... exponential ampliﬁcation. Primersequences used were as follows: cycD-F, 5¢-GGGATCCCACATTGTATTCG-3¢; cycD-R, 5¢-ACGGAGCTTTGAAGCCAGTA-3¢; cycE-F, 5¢-AAGGTGCAGAAGACGCACTT-3¢; cycE-R, 5¢-AATCACCTGCCAATCCAGAC-3¢;cdk4-F, ... develop-ment and gain insight into mechanisms of Jmj-medi-ated chromatin regulation, we have taken advantage ofDrosophila melanogaster as a model organism. Weshow here that loss of the Drosophila jumonji ... regulation, wehave taken advantage of Drosophila as a model organism. Drosophila Jmj(dJmj) shares high homology with mammalian Jmj in the JmjN, JmjC andAT-rich interaction domains, as well as in the...

Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx

... siRNA: sense, 5¢-AGGACUUCUUGAAAGGCAACAUUAAAG-3¢, antisense, 3¢-UAUCCUGAAGAACUUUCCGUUGUAAUU-5¢; scrambled HO-2 siRNA: sense,5¢-UAUAAGAGUCAGUACACAUCAUGGAAG-3¢,anti-sense, 3¢-UAAUAUUCUCAGUCAUGUGUAGUACCU-5¢.Another ... cDNAwere: forward, 5¢-AAGCTTCATGTCAGCGGAAGTGGAAAC-3¢; reverse, 5¢-CTGCAGTCACATGTAGTACCAGGCCAA-3¢. The sequence underlined is an artiﬁcialHindIII site. A full-length HO-2 cDNA fragment was iso-lated ... USA 101,1461–1466.50 Kimpara T, Takeda A, Watanabe K, Itoyama Y,Ikawa S, Watanabe M, Arai H, Sasaki H, Higuchi S,Okita N, et al. (1997) Microsatellite polymorphism in the human heme oxygenase-1...

Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

... S., Nakanishi, Y., Kodama, N., Takenaka, S., Shinke,R. & Aoki, K. (1998) Purification, characterization, and geneanalysis of catechol 2,3-dioxygenase from the aniline-assimilatingbacterium ... AB070889.RESULTSIdentification of a 4-amino-3-hydroxybenzoate-assimilatingorganismStrain 10d grew well in the basal medium containing4-amino-3-hydroxybenzoic acid and yeast extract andcompletely degraded the former ... 2,3-dioxygenase did not show signifi-cant levels of identity to sequences of other proteinsincluding those of extradiol dioxygenases available in theFASTA AND BLASTdatabase programs at the DNA DataBank...

Báo cáo khoa học: A strategy for the generation of speciﬁc human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx

... CCACCAGAACCTCCGCCTCCTGATCCGCCACCTCCTGAGGAGACGGTGACCAGGGTGCCJH3.link CCACCAGAACCTCCGCCTCCTGATCCGCCACCTCCTGAAGAGACGGTGACCATTGTCCCJH4-5.link CCACCAGAACCTCCGCCTCCTGATCCGCCACCTCCTGAGGAGACGGTGACCAGGGTTCCJH6.link CCACCAGAACCTCCGCCTCCTGATCCGCCACCTCCTGAGGAGACGGTGACCGTGGTCCCL. ... orientation.VK1.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGACATCCAGATGACCCAGTCTCCVK2.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGATGTTGTGATGACTCAGTCTCCVK3.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGAAATTGTGTTGACGCAGTCTCCVK4.link ... GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGAAATTGTGTTGACGCAGTCTCCVK4.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGACATCGTGATGACCCAGTCTCCVK5.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGAAACGACACTCACGCAGTCTCCVK6.link GGCGGATCAGGAGGCGGAGGTTCTGGTGGAGGTGGGAGTGAAATTGTGCTGACTCAGTCTCCVL1.link...

What is a Mouse-Trap Car and How does it Work? pdf

... surface friction will occur is between the axle and the chassis.The interface between the axle and the chassis is called the bearing. A plain bearing can be as simple as an axleturning in a drilled ... the cause; for example,avoid using aluminum as the axle or a bearing sleeve. A ball bearing is a set of balls in the hole which is arranged so that the axle rolls on the ballsinstead of sliding ... mouse- trap car is a vehicle that is powered by the energy that can be stored in a wound up mouse- trap spring. The most basic design is as follows: a string is attached to a mouse- trap’s lever arm and...

Báo cáo y học: "Genetic polymorphism of p53, but not GSTP1, is association with susceptibility to esophageal cancer risk – A Meta-Analysis"

... is worth mentioning that there are 2 main forms of esophageal cancer histologically, squamous cell car-cinoma (SCC) and adenocarcinoma, and each has dis-tinct etiologic and pathologic characteristics. ... performed stratified analysis ac-cording to ethnicity (Asian and Mixed/ Caucasian group). As shown in the Table 4, we found that the increased esophageal cancer risk associated with p53 Arg72Pro ... a larger and/or latest sample size was selected for the analysis. Data Extraction. Two investigators indepen-dently extracted data and reached a consensus on all of the items. Data extracted...

Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"

... immunostaining in spi-nal cord dorsal neurons was expressed using arbitrary units. Mouse Vgf radio immuno assay (RIA) C-terminal specific Vgf antibody (ab5901) was used in RIA analysis as previously ... immunocyto-chemically revealed that Vgf immunoreactive material in spinal cord motorneurons is already decreased in ~75 day old asymptomatic SOD-1 G9 3A- SOD1 ALS mice and continue to decrease as a function ... Paper Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis Zhong Zhao1,2, Dale J. Lange1,3 ,Lap Ho1,2,...

Tài liệu Is It the Network? Solving VoIP Problems on a Wireless LAN ppt

... Acknowledgmentsaccompany that data. In addition, Wi-Fi networks have a random backoff sequence that allows a wireless APand the stations that connect to it to share a wireless channel. Since a detailed discussion ... PMK caching is that the initial association to each AP on the wireless LAN still requires a full802.1X/EAP authentication so that a PMK can be created.This means that PMK caching only enables ... reserved.Page 3Once you begin analyzing delay limits rather than raw bandwidth limits, quantitatively determining a maxi-mum handset capacity per AP becomes impractical. Since the random backoff...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... is the His residue that in NirS is the proximal axial ligand to the d1heme.Replacement of an equivalent His, His41, in NirF byAla abolished binding of the heme to the protein. Known distal ... bifunctional dehydrogenase-ferrochelatasefrom Saccharomyces cerevisiae), we found that thetwo proteins had 24% sequence similarity. A crystalstructure of Met8P has shown that this protein hasan aspartate ... Isolation, sequencingand mutational analysis of a gene cluster involved in nitrite reduction in Paracoccus denitriﬁcans. AntonieLeeuwenhoek 66, 111–127.13 Kawasaki S, Arai H, Kodama T &...

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