Tài liệu Báo cáo Y học: Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Droge ¨ potx
Tài liệu Báo cáo Y học: Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Droge ¨ potx
... Use of site-specific recombination as a probe of nucleoprotein complex formation in chromatin Micha Schwikardi and Peter Dro¨geInstitute of Genetics, University of Cologne, GermanyDNA transactions ... targetsspanning 114 bp was impaired. This impediment wasmaintained over at least 72 h and was not affected by thetranscriptional status of chromatin nor by inhibitors of histonedeacetylases and ... DNA transactions such as transcription and site-specific recombination. Recently, chromatin remodeling and histone acetylation/deacetylation were identified as import-ant regulators of chromatin...
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Tài liệu Báo cáo Y học: The glucose-specific carrier of the Escherichia coli phosphotransferase system Synthesis of selective inhibitors and inactivation studies pptx
... [15]. In vitrophosphotransferase assaysPyruvate evolution was measured spectrophotometrically in 96-well microtiter plates at 30 °C, using the coupled assaywithL-lactate dehydrogenase and NADH. ... rate of inactivation by bromoacetyl-Glc ( 1a) was 15 times fasterthan by bromoacetic acid and 2.5 times faster than by thechemically more reactive iodoacetamide, suggesting somespecificity and ... is already dephosphorylated [6], as indicated by the complete inactivation induced byiodoacetamide.The bromoacetyl derivatives 1a and 1c also inactivatedGlc uptake by starved cells. Being...
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Tài liệu Báo cáo Y học: Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases pot
... 5¢-CCGAATTCTTAGGACCGACCGTTTTGG-3¢At173sense 5¢-GCGCTCGAAAATCTATTCAAGAACACC-3¢At173anti 5¢-GTTCTTGAATAGATTTTCGAGCGCACCGATGATAAC-3¢Bn173sense 5¢-GCCCTAGAAAATCTATTCAAGAACACC-3¢Bn173anti 5¢-GTTCTTGAATAGATTTTCTAGCGCACCATT-3¢At399sense ... 5¢-TTTAGAGACACTTGATTTGAGATGCGG3¢At114V93sense 5¢-CCGCATCTCAAATCAAGT GTCTCTAAA-3¢At114V95Ianti 5¢-CATGACTTTAGAGATACTAACTTTGAG-3¢At114V95Isense 5¢-CTCAAAGTTAGTATCTCTAAAGTCATG-3¢At114I105Vanti 5¢-ATCAGCTTTTCTTACTTGGAGAAAAT-3¢At114I105Vsense ... chain fatty acid; FAE1 KCS, fattyacid elongase-1 3-ketoacyl CoA synthase; KAS, 3-ketoacyl-acylcarrier protein synthase; cm-ura, complete minimal dropout medialacking uracil; FAME, fatty acid...
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Tài liệu Báo cáo khoa học: "Learning to Compose Effective Strategies from a Library of Dialogue Components" doc
... prerequisitefor task completion and user satisfaction.Typically, such systems are based on a dialoguestrategy that is manually designed by an expertbased on knowledge of the system and the domain, and ... assess,because the effectiveness of a strategy depends onmany different factors, such as classification/ASRperformance, the dialogue domain and task, and, perhaps most importantly, personality characteris-tics ... generation of (limited) exploratory dialogue data, using a train-ing system; creating an MDP from these data and the rewards assigned by the training users; off-linepolicy learning based on this MDP.The...
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Tài liệu Báo cáo Y học: The Fc receptor c-chain is necessary and sufficient to initiate signalling through glycoprotein VI in transfected cells by the snake C-type lectin, convulxin ppt
... of this signal, as cross-linking of GPVI in the absence of FcR c-chain was unable to promotean increase in phosphorylation of Syk. A transmembrane arginine residue and the cytoplasmictail of ... of Fura-2 emissions were measured and analyzed usingphycoerythrinFWINLABsoftware. Ratiometric analysis wasconverted into a concentration of Ca2+by measuring Rmax(maximal fluorescence in lysed, ... blotting as a band of 55 kDa.Additional bands of lower molecular mass are indicated. The associ-ated FcR c-chain was detected by Western blotting using a specificantibody. A sample of F1/c...
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Tài liệu Báo cáo khoa học: Bacterial-induced hepoxilin A3 secretion as a pro-inflammatory mediator pptx
... in ammatory bowel dis-ease because 12-LOX activity is induced at active sites of this disease.Because HXA3may play an important step underly-ing the pathophysiology of in ammatory diseases, such as ... MINIREVIEWBacterial-induced hepoxilin A 3secretion as a pro -in ammatory mediatorBeth A. McCormickDepartment of Pediatric Gastroenterology, Massachusetts General Hospital, and Department of ... toPseudomonas aeruginosa infection and HXA3, in turn,appears to mediate neutrophils transmigration acrossairway epithelial cells [27].Lung in ammationThe in ammatory response mounted against bacterialpathogens...
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Tài liệu Báo cáo khoa học: "Phrase-Based Statistical Machine Translation as a Traveling Salesman Problem" docx
... heuristic333estimate of the remaining cost for completing thetranslation. The variant which is mostly used is a form of beam-search, where several partial can-didates are maintained in parallel, and candidatesfor ... been topropose a transformation for an arbitrary phrase-based SMT decoding instance into a TSP instance.While certain similarities of SMT decoding and TSP were already pointed out in (Knight, ... was shown that any Traveling SalesmanProblem may be reformulated as an instance of a (simplistic) SMT decoding task, and while cer-tain techniques used for TSP were then adapted toword-based...
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Tài liệu Báo cáo khoa học: Use of hydrostatic pressure to produce ‘native’ monomers of yeast enolase ppt
... process, and Kdat0.1 MPa.Pressure inactivation of enolase was measured bysubjecting dilute solutions of enolase to pressure for varyingtimes, returning to 0.1 MPa, and immediately assaying thesample ... phospho-fructokinase [22], hexokinase [23], lactate dehydrogenase[24], and creatine kinase [25], have been examined by usingpressure. I n these examples, and others, pressure bothdissociated and inactivated ... protein, they are less hydrophobicthan the interior. In addition, several polar amino acids,especially arginine, lysine, glutamine and glutamate arefound more frequently at the interface than in...
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Tài liệu Báo cáo khoa học: "Use of Mutual Information Based Character Clusters in Dictionary-less Morphological Analysis of Japanese" ppt
... Use of Mutual Information Based Character Clusters in Dictionary-less Morphological Analysis of Japanese Hideki Kashioka, Yasuhiro Kawata, Yumiko Kinjo, Andrew Finch and Ezra W. Black ... in a 26- character alphabet, the domain of possible characters appearing in an average Japanese text is a set involving tens of thousands of characters, 658 2.1 Character Sort There are ... sense. Hiragana are basic Japanes e phonograms rep- resenting syllables. About fifty of them constitute the syllabary. Katakana are characters corresponding to hi- ragana, but their use is restricted...
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Tài liệu Báo cáo Y học: Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenol pyruvate carboxykinase determined by fluorescence spectroscopy pdf
... Chile;2Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, CanadaEscherichia coli phosphoenolpyruvate (PEP) carboxykinasecatalyzes the decarboxylation of oxaloacetate and ... binding; conformational changes; P-pyridoxylfluorescence spectroscopy.Escherichia coli phosphoenolpyruvate carboxykinase [PEPcarboxykinase; ATP:oxaloacetate carboxylase (trans-phos-phorylating) EC ... that theP-pyridoxyl group is a useful probe to monitor ligandbinding and ligand-induced conformational changes in E. coli PEP carboxykinase. The molecular model of theE. coli PEP carboxykinase...
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