... Shukla1,31 Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois, Chicago, IL, USA2 Department of Pathology, College of Medicine, University of Illinois, Chicago, ... performed as described previously. Theexpected sizes of the PCR products were 1270 bp for HVEM, 738 bp for nectin-1, 616 bp for nectin-2, and736 bp for 3-OST-3, respectively. HeLa cell cDNAs thatare ... was added for F-actin staining at room temperature for 45 min. Finally, thecells were washed three times with 1· NaCl ⁄ Pibefore micros-copy was performed using the 20· objective of the invertedmicroscope...