... 2,2,6,6-tetramethylpiperidin-1-yloxy free radical (TEMPO) asmediators, since they greatly differ both in their mechan-ism of action and in their specificity. In fact, HPI isrepresentative of a mediator ... activityThe activity of laccase was determined by following the rate of oxidation of 2,2¢-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) to ABTS radical cation (ABTS•+), byplotting the ... role of the enzyme, with the onlydifference of presenting fewer stringent steric and solubilityrequirements. The phenoxy radical of the product, in turn,should evolve towards end-products with...