... protein and mutants E142O and K13 3A. Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants K13 3A, E142O, W14 0A and W140O areshown in Fig. 3, with their thermodynamic ... mm NaHPO4⁄ 200 mm NaCl, pHadjusted to 7.0) at a concentration of 0.5 mgÆmL)1. Spec-tra were obtained as the average of five successivescans with a bandwidth of 1.0 nm and a scan speed of 20 ... thio-b-d-galactosidewere purchased from USB (Cleveland, OH). Salmon testesDNA and some analytical grade chemicals such as EDTA,Tris ⁄ HCl, CaCl2, NaCl and mineral oil were obtained fromSigma (St...