... protein, were associatedwith the anionic phospholipid liposomes of PS ⁄ PC,PtdIns ⁄ PC and PG ⁄ PC, and also the liposomes contain-ing phosphoinositides rather than zwitterionic liposomesof PE ... for 5 minand sonicated for 10 min, to prepare the PC-based lipo-somes [42,43]. ICand IC–CPY (2 lm final concentrations)were added to the liposome-containing solutions, and thesolutions were ... phospholipidrecognition.Using SPR sensorgrams for various concentrations(0.1–10 lm)ofIC, the membrane association rate con-stants (ka), dissociation rate constants (kd), and equi-librium dissociation constants...