Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

... Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity Laurent Volpon and Jean-Marc LancelinLaboratoire de ... antibiotics, as nystatins andamphotericins, are amphiphilic structures known to exert antifungal activity by disrupting the fungal cell membrane,leading to leakage of cellular materials, and cell death. ... nystatin A1 and rimocidin) and twoheptaenes (candidin and vacidin A) have been calculatedusing geometric restraints derived from1H -NMR data andrandom searches of their conformational space....

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... results of a phase II clinical trial with an alteredpeptide ligand. Nat Med 6, 1167–1175.22 Tselios T, Daliani I, Deraos S, Thymianou S, Matsou-kas E, Troganis A, Gerothanassis I, Mouzaki A, Mav-romoustakos ... highrelaxation with 5-DSA have relatively lower relaxationbecause of the presence of Fe3+. The apparent fastrelaxation of V7¢ in the presence of both paramagneticagents suggests that the ... Tzakos AG, Fuchs P, van Nuland NA, Troganis A, Tselios T, Deraos S, Matsoukas J, Gerothanassis IP &Bonvin AM (2004) NMR and molecular dynamics Structure of MBP immunodominant epitope C. Fare`s...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

... nonoxidative decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox-ylase. J Am Chem Soc 129, 9278–9279.20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas ﬂuorescens reveals a penta-coordinate mononuclear metallocofactor. J Am ... picolinicacid (PA), quinolinic acid (QA) and NAD homeostasis. Indeed, the enzymestands at a branch point of the tryptophan to NAD pathway, and deter-mines the ﬁnal fate of the amino acid, i.e. transformation...

Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc

... linkedto formation of an iron-peroxy adduct prone to fragmen-tation [266]. A similar p aradigm also appears to a pply to theﬁnal step in aromatase (CYP19)-catalyzed biotransforma-tion of androgens ... 11–813.119. Wada, A. , Ogo, S., Watanabe, Y., Mukai, M., Kitagawa, T.,Jitsukawa, K., Masuda, H. & Einaga, H. (1999) Synthesis andcharacterization of novel alkylperoxo m ononuclear i ron(III)complexes ... N-oxygenation of the tertiary arylamine at a rateless than half that of the wild-type-catalyzed reaction [142],so that reasonable interpretation o f the data seems d ifﬁcult.Evidence from comparative...

Tài liệu Báo cáo khoa học: The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol pptx

... 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢for the b subunit and 5¢-CATATGAGCGAGAAAACCATGCGCGTGCAG-3¢ and 5¢-AGATCTTAGCTTCCTTTACGCAGCTTATGC-3¢ for the c subunit. The segmentswere digested with NdeIandBglII ... Department of Bioscience andBiotechnology, Faculty of Engineering, Okayama University, Tsushima-naka, Okayama 700–8530, Japan.Fax: + 81 86 2518264, E-mail: toraya@biotech.okayama-u.ac.jpAbbreviations: ... and BglII to yield pUSI2E (a G). DNAsegments encoding the b and c subunits of glyceroldehydratase were ampliﬁed by PCR using pairs of primers5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢for...

Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 reﬁned models suggest environment-dependent induction of alternative bent ⁄extended resting states doc

... implementation of SOMO (SOlution MOdeller) inthe UltraScan analytical ultracentrifugation data analy-sis suite: enhanced capabilities allow the reliable hydro-dynamic modeling of virtually any ... ItalyIntroductionIntegrins are heterodimeric transmembrane (TM) cellu-lar receptors involved in mechanical anchoring andtwo-way signaling [1]. Each a and b subunit has a modular structure with a large extracellular ... tailseparation was in accordance with both the ET andhydrodynamic data of primed a IIbb3. Our revised mod-els further support an alternative view of the confor-mational states and mechanism of...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... alpha-sarcin: a study of relationships. Meth Enzymol 341,335–351.3 Lacadena J, A ´lvarez-Garcı´ a E, Carreras-Sangra N,Herrero-Gala´n E, Alegre-Cebollada J, Garcı´ a- OrtegaL, On˜aderra ... 2385involvement of a catalytic pair constituted by an acidand a base on each side of the hydrolyzed bond [16].E96 and H137 in a- sarcin and E58 and H92 in RNaseT1 act as the base and acid groups, ... assignment and structure calculation by cyana⁄ candid, a set of 20 structures thatsatisfy the experimental constraints was obtained. The Solution structure of hirsutellin A A. Viegas et al.2382...

Tài liệu Báo cáo khoa học: Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terriﬁcus venom docx

... distance data by dynamical simulated annealing from a random array of atoms. Circumventing problems associated with folding. FEBS Lett. 239, 129–136.31. Laskowski, R .A. , Rullmann, J .A. , MacArthur, ... theinactivation of the Na+channel in a fashion similar to that of scorpion a- toxins [11], other experiments have shown that, atlow doses, it has an analgesic activity involving both centraland ... that protein family.In addition, we show that both its fold and potentialsurface partially resemble the structural features of theantimammalian scorpion a- neurotoxins and of the humanantibacterial...

Tài liệu Báo cáo khoa học: Solution structure of the matrix attachment region-binding domain of chicken MeCP2 ppt

... duplex at molar ratios of 1 : 1.2 and of 1 : 2.4.Spectra analysis NMR data were analysed and processed with the computerprogramsXWINNMR,AURELIA[22], andAUREMOL[23](Bruker, Karlsruhe, Germany). ... ﬁnal part of theassignment of the NOESY spectra, the number of identiﬁedNOEs was increased by comparison of back-calculatedspectra with the experimental data [24,25]. Based on a preliminary ... rmsdvalues given in Table 1. Analysis of the Ramachandran plotshows that the dihedral angles / and w for the secondary structure elements are all found in the most favored or theadditionally allowed...

Tài liệu Báo cáo khoa học: Multi-targeted activity of maslinic acid as an antimalarial natural compound pdf

... acting at the terminal stages of haemoglobindegradation are also considered as potential antimalar-ial targets [22,23]. Other proteases related to the matu-ration of parasites and the invasive ... Slater AFG, Beavis R, Chait B, Cerami A & Henderson GB (1991) Hemoglobin degradation inthe human malaria pathogen Plasmodium falciparum :a catabolic pathway initiated by a speciﬁc protease. ... PE, Garcia-Granados A, Martinez A, Albericio F, Marquez N & Munoz E(2009) Solution- and solid-phase synthesis and anti-HIV activity of maslinic acid derivatives containing aminoacids and...

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