Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu pptx

... Functional expression and mutational analysis of ﬂavonol synthase from Citrus unshiu Frank Wellmann1,*, Richard Lukacˇin1,*, Takaya Moriguchi2, Lothar Britsch3, Emile Schiltz4 and ... been functionally proved and the lability of substrate and product rule out any cocrys-tallization. The setting of a helices and b-strands causes verysimilar CD spectra for this class of enzymes ... mutagenesis.MATERIALS AND METHODS Expression vectorThe FLS cDNA from satsuma mandarin fruits, C. unshiu [30], was excised with EcoRI and XhoI from the Bluescriptvector (Stratagene) and subcloned in...

Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

... The copy number of each variant in theunknown samples was determined from the calibrationcurves and data were normalized to the copy number of 18S RNA. Expression of ACMSD isoforms in E. colipGEM-ACMSD ... software swiss-pdbviewer and visual molecular dynamics [49].AcknowledgementsWe thank G. Magni and S. Ruggieri for helpful com-ments and suggestions and T. Begley (Department of Chemistry and ... ranging from 6.5 to 8.0, a Km of 6.5 lm, and a kcat of 1.0 s)1.ACMSD I is inhibited by quinolinic acid, picolinic acid and kynurenicacid, and it is activated slightly by Fe2+ and Co2+....

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... a kcat of 7.7 ± 0.9 s)1, Km of 0.148 ± 0.028 mm and kcat⁄ Km of 5.2 · 104m)1Æs)1toward shikimate, and akcat of 7.1 ± 0.7 s)1, Km of 0.182 ± 0.027 mm and kcat⁄ Km of 3.9 · ... shikimate, kcat of 7.1 s)1 and Km of 0.182 mm toward NADP, kcat of 5.2 s)1 and Km of 2.9 mm toward NAD. The optimum pH of theenzyme activity is between 8.0 and 9.0, and the optimum temperature ... infection.Results and DiscussionCloning, expression, and sequence analysis of HpSDHIn the current work, the aroE gene of H. pylori strainSS1 was cloned by using the genome sequences of H. pylori...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... Puriﬁcation and cDNA cloning of a cellulase from abaloneHaliotis discus hannaiKen-ichi Suzuki, Takao Ojima and Kiyoyoshi NishitaLaboratory of Biochemistry and Biotechnology, Graduate School of Fisheries ... phosphate (pH 7.0), and an appropriate amount of enzyme at 30 °C. Thereducing sugar liberated by hydrolysis of CMC wasdetermined by the method of Nelson and Somogyi [25].One unit of cellulase was ... reverse primers R1 and R2 weresynthesized on the basis of sequences of LP5 and LP6,respectively (Table 2).PCR ampliﬁcation of HdEG66-cDNAcDNAs encoding the N-terminal region of the HdEG66were...

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... ﬂuorescence intensity as function of time of double-labeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-IIorLPSReatt = 50 s and subsequent addition of LBP(0.2 mM)att = 100 ... were kind gifts of K H. Wiesmu¨ller1(Tu¨bingen,Germany). Bovine brain 3-sn-PS and egg 3-sn-PC wereobtained from Sigma (Deisenhofen, Germany). For pre-paration of liposomes from a phospholipid ... respectively [23,30], and the bands at1172, 1085, and 1038 cm)1assigned to glucose ringvibrations [31]. As no additional bands in the range of theamide vibrations centered at 1650 and 1550 cm)1can...

Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx

... theactivation of Ras and ERK1 ⁄ 2 downstream of GPCRs, Ras-GRF1 could be one of possibly severalGEFs involved in the activation of Ras and subse-quently ERK1 ⁄ 2 downstream of Gs-coupled ... Research Council of Norway, The Novo Nor-disk Foundation, Anders Jahre’s Foundation for thePromotion of Science, The Blix Family Foundation and grants from the University of Oslo and the NationalCancer ... indicatedperiods of time (Fig. 2). Serotonin treatment increasedthe phosphorylation of the endogenous 140 kDa and  60 kDa isoforms of Ras-GRF1 (Fig. 2A, left panel) and of recombinant HA-Ras-GRF1...

Tài liệu Báo cáo khoa học: Marine toxins and the cytoskeleton: pectenotoxins, unusual macrolides that disrupt actin pptx

... beenisolated from the digestive glands of scallops collectedin Japan, and these are stereoisomers of PTX-1 and PTX-6, respectively. After acid treatment of PTX-4 and PTX-7, two isomers, named PTX-8 and ... interactions leadsto a cessation of new filament growth and a disruption of existing filaments, and is accompanied by a loss of motility, breakdown of adherens junctions, polyploidy and, ultimately, apoptosis ... form. Epimerization of PTX-2 SA yieldsthe thermodynamically more stable 7-epi-PTX-2 SA, and both have been detected in shellfish from Portugal[6], Ireland [2], New Zealand [4] and Croatia [3].Besides...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... graph of R1, R2 and {1H}–15N NOE val-ues along the sequence of the TTF1 HD at 11.7 T and 286 K.Fig. S2. Plot of J(0.87xN)–J(0) correlation from 15Nrelaxation measurements of the ... and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major cluster,towards the lower intercept of the theoretical curve and ... amplitudes and slow rates and, con-versely, large amplitudes and faster rates, for theinternal motions along most of the structured core of the molecule. This picture is physically inconsistent,and...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different residues. It ... system [48]. The visual analysis of cono-marphin was done using molmol [49] software, and thegeometric qualities of the obtained structures were assessedwith procheck-nmr software [50].AcknowledgementsThis ... structure of conomarphinwas similar to those of other conotoxin cDNAs. ThecDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 resi-dues, a proregion of...

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... nature of the molecule, as assessed from the salt and heparin dependence of the antimicro-bial and antifungal effects.ResultsAntimicrobial effects of recombinant wild-typetrappin-2 and trappin-2 ... (2006)Secretion of serine peptidase by a clinical strain of Candida albicans: inﬂuence of growth conditions and cleavage of human serum proteins and extracellularmatrix components. FEMS Immunol Med ... exacerbations of COPD. Infection 35, 143–149.38 Higuchi R, Krummel B & Saiki RK (1988) A generalmethod of in vitro preparation and speciﬁc mutagenesis of DNA fragments: study of protein and DNA...

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