... Ventura1,21Department of Biomedical Sciences, Division of Biochemistry, Laboratory of Cardiovascular Research,2Division of Cell Biology,National Institute of Biostructures and Biosystems, and 3Department of ... directed against human sequencesfor c-myc and GAPDH and PCR conditions were aspreviously described [20].Analysis of c-mycgene transcription by nuclear runoffassayTo prepare ECV304 nuclei, cells ... mediated, at least in part, by G-protein coupled receptors (GPCRs), stimulation of membrane phospholipid hydrolysis, and activation of thediacylglycerol sensitive isoforms of protein kinase C (PKC)[28]....