Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

... notplay a determinative role in conferring the structure of AII. X-ray structure of AII bound to the mAb Fab131: comparison with the NMR solution structure The phenomenon of conformational stabilization ... X-ray structure of AII complexed to the Fab131 mAb. In Fig. 7A, asequence alignment is represented of the backbone of the conformational ensemble of AII in solution state with the backbone of the X-ray structure. ... stimulation of the receptor and transformation to the signalling state [62].2 The present NMR data provide the basis for a quantitativecomparison of the structure of AII in solution with the X-ray structure...

Tài liệu Báo cáo khoa học: NMR structural characterization of HIV-1 virus protein U cytoplasmic domain in the presence of dodecylphosphatidylcholine micelles doc

... implications of the observed VpUcyt structure One of the most studied activities of VpU is the induc-tion of CD4 degradation in the endoplasmatic reticu-lum of infected CD4+cells. Only the cytoplasmicdomain ... preliminaryprotein structures [79]. radar runs in an iterative manner. The input of the ﬁrst cycle consisted of the 3D NOESYdata, the amino acid sequence of VpUcyt and the chemicalshift list reﬂecting the ... (2002)Structural investigation of the binding of a herpesviralprotein to the SH3 domain of tyrosine kinase Lck.Biochemistry 41, 5120–5130.M. Wittlich et al. NMR structure of micelle-associated HIV-1...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... invasion. The concerted process of opening of the initial double strand and the annealing of the new duplex ﬁnally results in either the replacement of the original strand or the expulsion of the invadingstrand, ... entropy increase of the system drives binding of the peptide to the RNA (and thus, indirectly, the acceleration of annealing). However, the extent of decrease of annealing acceleration caused by the ... contain the canonical binding motif. Awell-studied example is the CBP2 protein from yeastmitochondria, which binds speciﬁcally to the bI5group I intron [32]. The interaction of CBP2 with the intron...

Tài liệu Báo cáo khoa học: NirF is a periplasmic protein that binds d1 heme as part of its essential role in d1 heme biogenesis pdf

... Asp141 of Met8P. The idea of NirF being a dehydrogenase is appealingbecause of the presence of a putative nucleotide-bind-ing motif in the N-terminal of the protein sequenceand also because there ... activity of NirFInterestingly, like NirN, NirF shares sequence similar-ity with the C-terminal d1heme-containing domain of cytochrome cd1. Strikingly, the axial ligand of iron in d1heme in ... and of the kanamycin insertion mutant nirF strain(SBN03) in the upper right panel. Growth of SBN11 (DnirF) is shown in the bottom leftpanel and of SBN13 (DnirF) containingpEG276-nirF in the...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... stimulates intrinsic tyrosine kinase activity of the receptors and triggers autophosphorylation of speciﬁc tyrosine residues within the cytoplasmic regula-tory domain.These phosphorylated tyrosines ... signaltransducers then bind to these adaptors to initiate mul-tiple signalling pathways, including mitogen-activatedprotein kinase, phosphatidylinositol 3-kinase ⁄ AKT and the signal transducer and activator ... extracellulardomain is subdivided further into four domains. The tyrosine kinase domain consists of an N-lobe and aC-lobe, and ATP binds to the cleft formed betweenthese two lobes. The C-terminal regulatory...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... result in repositioning of critical residues surrounding the ATP-binding cleft of the tyrosine kinase domain of the receptor and therebystabilize the interaction with EGF-TKIs, leading to anincrease ... mutations result in reposition-ing of critical residues surrounding the ATP-binding cleft of the tyrosine kinasedomain of the receptor and thereby stabilize the interaction with EGF-TKIs.Table ... binding to the tyrosine kinase pocket of the receptor, therebyinhibiting receptor tyrosine kinase activity and EGFRsignaling pathways (Fig. 1). Early clinical studiesshowed that a subset of...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx

... splicing of the E2-responsivegene, oxytocin [90]. NRs also interact with the SR(serine- and arginine-rich proteins) family of splicingfactors. CAPER, a SR-like protein, interacts with ERaand the ... promoters of cathepsin D and pS2along with ERa in an E2-dependent manner. In addition to the direct interaction of MLL2 with ERa, MLLs may interact with ERs via other MLL-interacting proteins (NR-box ... other binding to the promoter of NR target gene.Thus, these studies suggest that in addition to directinteractions of MLLs with NRs, they interact via vari-ous MLL-interacting components in...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... proteins. Thus, the MEN1 ⁄ LEDGF-interactingdomain linked to DNA-binding domains (AT-hook andMT domain) becomes disconnected from the PHDdomains, the FYRN domain, the transactivatingdomain, the ... elongation of HIV-1 is regu-lated by the TAT ⁄ TAR ⁄ P-TEFb system [38]. Therefore,CDK9 inhibitors may be a promising tool with which to gain insight into the molecular mechanisms of MLL-mediated ... sig-naling [44]. In line with this, GSK3 has recently beenidentiﬁed in a complex with DDX3 and cellular inhibi-tor of apoptosis 1 that prevent apoptotic signaling viacompetitive binding to death...

Tài liệu Báo cáo khoa học: Anaerobic sulfatase-maturating enzyme – A mechanistic link with glycyl radical-activating enzymes? docx

... assigned in the central part of these proteins (Fig. 6). These proteinsare the activating enzyme involved in quinohemopro-tein amine dehydrogenase biosynthesis, which isinvolved in the cross-linking ... [28,29], and the increase in spin quantiﬁcation is likely to be a conse-quence of the increase in redox potential that resultsfrom AdoMet binding [30]. In contrast, within the lim-its of experimental ... function of the cysteinyl clusters of the anSMEs [25]. They made single amino acid substitu-tions into the three conserved cysteinyl clusters of anSMEkp and co-expressed the corresponding mutantsin...

Tài liệu Báo cáo khoa học: Arabidopsis thaliana BTB⁄ POZ-MATH proteins interact with members of the ERF⁄AP2 transcription factor family ppt

... interface [8,9]. BTB ⁄ POZ proteins areoften transcriptional regulators containing a C2H2domain for DNA binding, but can also be found in combination with various other protein–protein inter-action ... proteins use their BTB ⁄ POZ domain to interact with the cullins CUL3A and CUL3B [3,4,16], we inves-tigated what kind of protein–protein interactions werefacilitated by their MATH domains by ... proRAP2.4:GUS lines showed GUS expression in 3-day-old seedlings in cotyledons and in the central cylinder of the root, but not in the lower parts of the hypocotyl (a). Rosette leaves werestained in the...

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