Tài liệu Báo cáo khoa học: "The Effect of Pitch Accenting on Pronoun Referent Resolution" ppt

... attentional salience. One determiner of whether attentional or propositional effects are dominant is the type of information provided by the accented constituent. Because nonpronominals contribute ... Effect of Pitch Accenting on Pronoun Referent Resolution Janet Cahn Massachusetts Institute of Technology Cambridge, MA 02139 USA cahn~media.mit.edu Abstract By strictest interpretation, ... corresponding propositional effect as described in PH90. The corollaries for pitch accented pronominals are: (1) when a pitch accent is applied to a pronominal, its main effect is attentional, on...

Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf

... Arg-HClin the absence of the chaperone precluded analysis of the effect of this concentration on the protectiveability of aB-crystallin.Reduction-induced aggregation of the insulin B-chainLight ... incubated alone (Fig. 2A,C). Addition of Arg-HCl(up to 250 mm) had a negligible effect on the amount of precipitation. Similarly, low and intermediate con-centrations of Gdn-HCl had no effect on the ... Inaddition, for each assay we used concentrations of aB-crystallin that only partially inhibited aggregation of the target protein in order to enable the effects of the compounds on the chaperone...

Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx

... addition of 100 lLof2· Kirby buffer [25] and 80 lLofH2O, andrRNA was obtained by precipitation after two phenol–chloroform extractions. Four micrograms of rRNA weretreatedwith20lL of acetic-aniline ... effect of substitutionsmade at each of the arginyl residues on catalytic activity of RTA, the ability of each of the puriﬁed RTA variants todepurinate yeast ribosomes was compared to that of ... low expression levels and puriﬁcation of these variantswas not achieved. However, although a very low expressionlevel was again seen on substitution of Asp75 to Ser,substitution of Asn78 to...

Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx

... strictly conserved. A potential N-glycosylation site(NXS ⁄ T), found in PmaHc2 at position Asn334, isconserved in all subunit types (Hc1 and Hc2) with theexception of PmaHc1. An insertion of nine ... hemocyanins are only expressedunder certain environmental conditions or in somedevelopmental stages.Putative function of hexapod hemocyaninsReversible binding of oxygen and hence function as arespiratory ... have long been considered unnecessary [10–12]. Nevertheless, a functional hemocyanin has beenidentiﬁed in the hemolymph of the stoneﬂy Perla mar-ginata [8]. This hemocyanin consists of two...

Tài liệu Báo cáo khoa học: The sequentiallity of nucleosomes in the 30 nm chromatin ﬁbre pptx

... sedimentationproﬁles after 100%, 90% or 80% chemical crosslinking of nucleosomes and digestion of the DNA to mononucleosome size of samples con-sisting of a mixture of hepta-, octa- and nonanucleosomes ... 5-mers). At longer times of digestion, morethan 50% of the sample was converted into the frac-tion S. Breaking the disulfate bond in the middle of the crosslinker produced only mononucleosomes ... digestion times caused an increase of themononucleosome fraction without appearance of di- topenta-nucleosomes (Fig. 4C,D,E) and all the oligonu-cleosome fractions appeared only after a long...

Tài liệu Báo cáo khoa học: The stereochemistry of benzo[a]pyrene-2¢-deoxyguanosine adducts affects DNA methylation by SssI and HhaI DNA methyltransferases pptx

... concentrations of B[a]P-N2-dG adducts were compared with the levels of DNA methylation, and thus the effect of the abso-lute conﬁgurations and conformations of the adducts on DNA methylation was ... (+)-cis-B[a]P-N2-dG adduct conforma-tions, it is of structural interest to compare the effects of these conformations on DNA methylation. In thiswork, the effect of the intercalated [9] (+)-cis-anti-B[a]P-N2-dG ... almostindependent of the position of the damaged guanineresidue X+.DiscussionThe goal of our study was to elucidate the effect of stereochemistry and adduct conformation of (+)-cis-B[a]P-N2-dG...

Tài liệu Báo cáo khoa học: The localization of FGFR3 mutations causing thanatophoric dysplasia type I differentially affects phosphorylation, processing and ubiquitylation of the receptor pptx

... termination codon had mild effects on receptor phosphorylation and glycosylation,whereas conversion of Lys650 into methionine inducedstrong constitutive phosphorylation of the native non-glycosylated ... intracellular retention. Bycontrast, TDI mutations in the ECD or disruption of the termination codon induced a much lower level of phosphorylation of only the fully glycosylated isoform,which did ... use of retroviruses for stable transfection of cDNA constructs versus transient transfection of plas-mids. Polyubiquitylation is responsible for the internal-ization and proteasomal degradation...

Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx

... generate the set of orientations forDELPHI calcula-tions through rotation of the RNase around its major axis (rotationangle ‘r’) and variation of the inclination (inclination angle ‘i’) withrespect ... underthe conditions used in the experiments with mem-branes, hence their different effects on the model mem-branes may not be ascribed to different conformations of the three proteins.Effects on ... (2001) Preparation of potent cytotoxic ribonucleases by cationization:enhanced cellular uptake and decreased interaction withribonuclease inhibitor by chemical modiﬁcation of carboxyl groups....

Tài liệu Báo cáo khoa học: The role of antioxidants in the cytotoxicity of chemotherapeutic drugs pptx

... modiﬁca-tions function. Our focus recently has been the analysis of his-tone methylation at lysines and arginines. We now ﬁnd that onesite of lysine methylation on histone H3 is under the control of proline ... robust inhibition of liver VLDL production, transcriptional suppression of hepaticglucose production, abrogation of non-alcoholic steatohepatitis,activation of glucose uptake during hyperinsulinemic–euglycemicclamp, ... functions of adipocytokine secretion might be regulateddynamically by nutritional state. Visceral fat accumulation causesdysregulation of adipocyte functions, including oversecretion of TNF-a,...

Tài liệu Báo cáo khóa học: The lysozyme of the starfishAsterias rubens A paradigmatic typei lysozyme docx

... available online at http://smi.snv.jussieu.fr/hca/. To get three-dimensional representations of catalyticcentres of lysozyme i and compare it with that of lysozymesc, the primary sequences of Asterias ... four-residue insertion(residues 55–58 on Fig. 3). Comparison with the secondexon of the human lysozyme c that comprises the active sitereveals both similarities between the two types of lysozymesand ... Tapesjaponica; Hme, H. medicinalis. Numbering is that of the starﬁsh lysozyme. For comparison, the second exon of the human lysozyme-c (Has, Homosapiens) is also aligned. Below are noted secondary...

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