... There-fore, these results clearly indicate that the electrontransport system for CYP175A1 is composed of Fdx,FNR, and NADPH (Fig. 4B).All quantitative analyses were performed at 65 °C for 2 ... via OFOR and Fdx to CYP119 [13,14]. Inter-estingly, the electron transport system for CYP175A1did not utilize OFOR, although the T. thermophilusHB27 genome contains the genes encoding OFOR(TTC1591 ... 5¢-GGAATTCCATATGAAGCGCCTTTCCCTG-3¢ (forward primer) and 5¢-CCAAGCTTTCACGCCCGCACCTCCTCCCTAG-3¢ (reverse primer). PCR was carriedout at 94 °C for 5 min, and this was followed by 30 cyclesof 94 °C for 30 s, 55 °C for 30...