Tài liệu Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance pptx

... crossover intermediate is thereforecrucial for the integrity and maintenance of DNA in allorganisms including mitochondrial DNA (mtDNA). Mitochondrial DNA amounts to about 15% of the DNA content in ... base-pairs in the centre of the junctions [19]. Detailedunderstanding of the functional role of these interactionswould require the crystal structure of the Ydc2 junction complex. In this study the ... [14,20]. Binding of CCE1 results in the complete unpairing of the four base-pairs in the centre of the junctions with a lesser disruption of the next base-pairs[19], which is reminiscent of the structure...

Tài liệu Báo cáo khoa học: Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation ppt

... mBmal1 cDNA as atemplate. The cDNAs thus obtained were subcloned into the mammalian two-hybrid vector pBIND (Promega) forexpression of the GAL4 DNA- binding domain (DNA- BD)fusion protein.Luciferase ... C-terminal region, including the PAS-Bdomain of BMAL1, is required for the binding to DEC1,Fig. 4. Identiﬁcation of the domain of D EC1that interacts with BMAL 1. Interactions of various DEC1 ... eletion of 111 amino acids f rom the N-terminal region (BMAL1:112–626) did not affect the binding of BMAL1 to DEC1; deletion of 235 amino acids(BMAL1:236–626) slightly diminished the binding,although...

Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

... serine prote-ase located in the amino-terminal domain of NS3. Ser-ine protease activity of NS3 requires NS4A, a proteinconsisting of 54 amino acid residues, to form a stablecomplex with the ... factors, including IFN regulatory fac-tor-3 (IRF-3) and kinases, including the Tank-bindingkinase 1 (TBK-1) and inhibitor of j B kinase e (IKK-e)[28–31]. Although IRF-3 is located in the cytoplasm ... [8–10].Virus-infected cells trigger the innate immuneresponse by recognizing viral components, including DNA, ssRNA, dsRNA and glycoproteins. Thisresponse initiates signaling pathways leading to the induction...

Tài liệu Báo cáo khoa học: Functional characterization of an orphan cupin protein from Burkholderia xenovorans reveals a mononuclear nonheme Fe2+-dependent oxygenase that cleaves b-diketones ppt

... byxanthine oxidase was followed indirectly by measuring the increase in absorbance at 550 nm resulting from cyto-chrome c being reduced by superoxide in the course of the reaction. The principle of ... catalysis, ligand binding, and the role of storage proteins, the cupins constitute one of the mostdiverse superfamilies of proteins known. They havebeen described from all three domains of life [1,2], ... used to examine the coordination of Fe2+ in the active site of the resting enzyme. The best fit of the extended X-ray absorption fine structure (EX-AFS) data indicated a five-coordinate or six-coordi-nate...

Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate speciﬁcity docx

... in the size of the side chain may create steric hindrancefor the substrate, making the base moiety of the sub-strates bind in a nonoptimal conformation.Another interesting feature concerning ... ion and arginines of the Lid-region sta-bilize the negative phosphates of this state. The moststriking effect of mutation of arginine to histidine is analmost 2000-fold decrease in the kcatvalue ... acids lining thisspace. Binding of BVDU to Dm-dNK-Y70W compen-sates for the loss of one hydrogen bond to 3¢-OH by atighter ﬁtting of the bromovinyl group, thus restoring the tight binding lost...

Tài liệu Báo cáo khoa học: Functional hierarchy of plasminogen kringles 1 and 4 in ﬁbrinolysis and plasmin-induced cell detachment and apoptosis docx

... Llinas M (1988) Lysi-ne ⁄ fibrin binding sites of kringles modeled after the structure of kringle 1 of prothrombin. Proteins 3, 85–96.20 Thorsen S (1975) Differences in the binding to fibrin of native ... specifically inhibit the LBS function of either K1[34] or K4 [35] and have used them to analyse, in functional isolation, the role of these LBS on plas-min(ogen) binding and activation. The effect of thesemAbs ... was investigated by selectiveneutralization of their lysine-binding function. Blocking the kringle 1 lysine-binding site with monoclonal antibody 34D3 fully abolished binding andactivation of...

Tài liệu Báo cáo khoa học: Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts pptx

... show the positions of the protruding coiled-coil motif (residues 187–226) and the C-terminal module. The terminal positions of the deletion of the coiled-coil motif and the insertion of the linker ... rates in the presence of kirromycin The effect of kirromycin on the growth of GRd.tsf wasinvestigated by measuring growth rates of cultures in LBcontaining various concentrations of kirromycin. ... Growth in the presence of kirromycin. The growth rate of strainGRd.tsf is presented as a percentage of the corresponding growth rate of the wild-type strain, UY211, in the presence of kirromycin.Fig....

Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... excluded all residuesinvolved in the binding of the metal cofactor.ApeSODs in different forms were superimposed with the least square ﬁt of Ca atoms of the residues in the range10–200. ApeSOD ... focuson the coordination of the metal cofactor in the activesite as well as the changes it experiences in response todifferent metal cofactors. Finally, by comparing Ape-SOD with the SOD from the ... can bind each other’s metals, they are inactivewhen the wrong metal is incorporated at the active site[3]. However, several SODs are active in the presence of either Fe or Mn. These types of...

Tài liệu Báo cáo khoa học: Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese-metallocofactor (R2F) and a stable tyrosyl radical (Y•) involved in ribonucleotide reduction ppt

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... the increase of the concentration of the organic radical in response toadded manganese indicates an obligatory role of thismetal during in vivo generation of the radical. It isexpected that the ... from the R1 subunit back to the tyro-sine Y122 of the R2 subunit; and (d) dedocking of the product deoxyribonucleotide and reduction of the disulﬁde cysteine by NADPH. In in vitro stud-ies, the...

Tài liệu Báo cáo khoa học: Crystal structures of the regulatory subunit of Thr-sensitive aspartate kinase fromThermus thermophilus pdf

... around the Thr-binding site is missing in TtAKb-free, and that b-strands surrounding the Thr-binding site show outward shifts in the absence of Thr, with 12° rotation of the ACT2 domain from the ﬁxed ... stick model. Both ACTdomains forming an effector-binding unit of the front of the dimer are indicated. Site 1and site 2 of the effector-binding unit on the front are indicated by solid and dotted ... A chain, and residues in green are in the B chain. (C) Vacant Thr-binding site in TtAKb-free. Residues in blue are in the E chain, and residues in orange are in the F chain. (D) Structure-based...

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