Tài liệu Báo cáo khoa học: Gene transcription of fgl2 in endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 pdf

... studies in Drosophila Schneider cells conﬁrmed that the constitutiveexpression of this gene is controlled by Ets-1 and requires the presence both of the Sp1 and Sp3 transcription factors. The presence ... endothelial cells is controlled by Ets-1 and Oct-1 and requires the presence of both Sp1 and Sp3 Mingfeng Liu1, Julian L. Leibowitz2, David A. Clark1,4, Michael Mendicino1, Qin Ning1, Jin Wen ... its motif. Ets-1 is critical in regulating fgl2 expression and requires the presence of both Sp1 and Sp3 Collectively the linker-scanning and EMSA analysis suggestthat Oct 1, Sp1/ Sp3, Ets family...

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... immunoblot analysis with the anti-c-Myc IgG. The intensity of the band of the proteasedomain was quantiﬁed using the protease domain of the protein obtained from E. coli .Enzyme inhibition assayHAI-1–K1 ... tissuesproducing it. In response to tissue injury, such ashepatic and renal injury, the inactive single-chain HGF is converted to a two-chain form exclusively in the injured tissue. This conversion is ... two-chain form in vitro [31]. The role of HGFA in the pro-teolytic activation of HGF in vivo was determined by analysing knockout mice. In HGFA-deﬁcient mice,regeneration of the injured intestinal...

Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx

... 2007 FEBStolerate lack of ALK3 in absence of the inhibitor.Although functional proof is missing, the authorsfound up-regulation of ALK1 and ALK2 in these cells and suggest these receptors to be ... occurs outside of the signal receiving cell, by secretion of blockingligands such as Noggin, Chordin and Follistatin orGDF3. Another way to modulate ligand ⁄ receptor inter-action is by receptor ... member of the bHLH transcription factor family, which can beefﬁciently bound and thereby inactivated by ID pro-teins. In the rat tumour cell line AR42J, derived from the acinar pancreas, and isolated...

Tài liệu Báo cáo khoa học: Yeast glycogenin (Glg2p) produced in Escherichia coli is simultaneously glucosylated at two vicinal tyrosine residues but results in a reduced bacterial glycogen accumulation docx

... protein band from the patterns obtained a t the beginning (Glg2p0) and from the end of the incubationperiod (Glg2p20) were excised and digested with trypsin in the gel. T he resulting peptide ... of these two enzymes does not permit the de novo synthesis of aglucan. Both in fungi and in animals glycogen biosynthesisappears to b e initiated by the action of another U DPglucose-dependent glucosyltransferase, ... that interacts with the mamma-lian glycogenin and thereby enhances the self-glucosylatingactivity [14,15]. In prokaryotes the initiation of glycogen b iosynthesis hasnot yet been elucidated. The...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer ppt

... domain; and aC-terminal regulatory domain [2]. The extracellulardomain is subdivided further into four domains. The tyrosine kinase domain consists of an N-lobe and aC-lobe, and ATP binds to the ... bind to EGFR and ERBB4 (including betacellulin, heparin-binding EGF and epiregulin); and (c) neuregulin (NRG) (also knownas heregulin) that binds to ERBB3 and ERBB4.NRG1 and NRG2 bind to both ... ligands, dynamic conformational changes occur in both extra-cellular and intracellular domains of the receptor tyrosine kinases, resulting in the transphosphorylation of tyrosine residues in the...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in gene expression, hormone signaling and mRNA processing docx

... transactivation domain. The DNA-bind-ing domain is responsible for DNA binding specificity and dimerization, and the ligand-binding domain is responsible for binding of the ligand and associatedinduced ... distinct subset of cofactors, depending on the target gene, binding affinities and relative abun-dance of these factors in the cells [65]. These coactiva-tors and repressors usually exist in ... DNA-binding domains, protein–proteininteraction modes and pre-existing chromatin modifica-tions may facilitate the binding of MLLs, depending on the context and cell types, to facilitate transcription activation....

Tài liệu Báo cáo khoa học: Gene silencing at the nuclear periphery pdf

... concerningmechanisms of gene repression by proteins of the NE and suggest the hypothesis that the epigenetic ‘histone code’, dictating transcriptionalrepression, is ‘written’ in part, at the NE by ... Lining the nucleoplasmic side of the NE, and in close contact with it, is the nuclear lamina (reviewed in [10,10a]). It is a protein meshwork composed of lamins and a growing number of NE lamin ... from the possi-bility of single lysine and arginine residues to undergomono-, di- or tri- (in the case of lysine) methylation. The histone code in uences the structure of the chro-matin ﬁber...

Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx

... invasion. The concerted process of opening of the initial double strand and the annealing of the new duplex ﬁnally results in either the replacement of the original strand or the expulsion of the invadingstrand, ... determines the magnitude of peptide–RNA binding, owing tocounterion release from the RNA backbone [66]. The resulting entropy increase of the system drives binding of the peptide to the RNA (and ... but is in fact a prerequisite for the chaperone and annealing activity, and that it is the key to understanding the mechanism of protein-facilitated RNA folding. To develop this idea further,we...

Tài liệu Báo cáo khoa học: Epidermal growth factor receptor in relation to tumor development: EGFR-targeted anticancer therapy doc

... mutations result in reposition-ing of critical residues surrounding the ATP-binding cleft of the tyrosine kinasedomain of the receptor and thereby stabilize the interaction with EGF-TKIs.Table 2. ... tyrosine kinase domain of the receptor and therebystabilize the interaction with EGF-TKIs, leading to anincrease of  100-fold in sensitivity to inhibition by EGFR-TKIs compared with that of the ... signaling pathways beingturned off and the cancer cells undergoing apoptosis.Moreover, EGFR mutations result in repositioning of critical residues surrounding the ATP-binding cleft of the tyrosine...

Tài liệu Báo cáo khoa học: Mixed lineage leukemia: roles in human malignancies and potential therapy pdf

... proteins. Thus, the MEN1 ⁄ LEDGF-interactingdomain linked to DNA-binding domains (AT-hook and MT domain) becomes disconnected from the PHDdomains, the FYRN domain, the transactivatingdomain, the ... the presence of Meis1 and Pbx proteins,as well as on the presence of Men1 and Ledgf [19–21].More recently, it has been demonstrated that overex-pressed Meis1 results in the establishment of ... unique gene- expression signature that is further enhanced by the presence of the HoxA9 protein [20]. Men1 bindsdirectly to the N-terminus of MLL fusions [22] and was essential for MLL fusion proteins...

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