Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

Tài liệu Báo cáo khoa học: Characterization of 1H NMR detectable mobile lipids in cells from human adenocarcinomas doc

... Antonella Rosi1, Claudio Giovannini2,Orazio Sapora3, Vincenza Viti1 and Laura Guidoni11 Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanita`, Istituto Superiore di ... phosphatidylcholine synthesis is reduced orin the presence of phosphatidylcholine degradation[18,25]. In particular, inhibition of the ﬁnal step of phosphatidylcholine synthesis via the Kennedy ... Sanita`, Rome, Italy2 Dipartimento di Sanita`Pubblica Veterinaria e Sicurezza Alimentare, Istituto Superiore di Sanita`, Rome, Italy3 Dipartimento di Ambiente e Connessa Prevenzione Primaria,...

Tài liệu Báo cáo khoa học: Characterization of chitinase-like proteins (Cg-Clp1 and Cg-Clp2) involved in immune defence of the mollusc Crassostrea gigas docx

... classiﬁcation, based only onsimilarities in amino acid sequences, groups togetherchitinases and proteins devoid of catalytic activity due to the substitution of a critical amino acid in the cata-lytic ... transcripts duringdevelopment and in adult tissues To gain insights into possible physiological functions of Cg-Clp2, determination of its tissue distribution and temporal pattern of expression ... the resorption of gonadic tubules and the rebuilding of storage tissues [22]. The expression of Cg-Clp2 duringthis particular period is somewhat reminiscent of the ﬁnding that certain mammalian CLPs...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... expression in vivo orin vitro, including characterization of the promoterregion of the gene and the associated transcriptionalfactors. Therefore, delineation of the transcriptionalregulation of ... 1a.ResultsMapping of transcription initiation sites in the human DNASE1 gene To identify the transcription initiation sites of DNASE1 in pancreas, 5¢-RACE based on RNA ligase-mediated and oligo-capping ... transcriptional regulationYoshihiko Kominato1, Misuzu Ueki2, Reiko Iida3, Yasuyuki Kawai4, Tamiko Nakajima1, ChikakoMakita1, Masako Itoi1, Yutaka Tajima1, Koichiro Kishi1 and Toshihiro...

Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc

... Maria Ida De MichelisDipartimento di Biologia ‘L. Gorini’, Universita`di Milano, CNR Istituto di Bioﬁsica – Sezione di Milano, Italy The H+-ATPase is the major electrogenic pump in the plasma ... C-terminus of isoform 1 of the PM H+-ATPase of A. thaliana (AHA1) interacts with the ﬁrst 88 aminoacids of PPI1 [22], indicating that the PM H+-ATPase binding site of PPI1 is localized therein. ... stimu-lates its activity [22]. The available preliminary evidenceindicates that its mechanism of action is different fromthat of 14-3-3 proteins, the best known modulators of the autoinhibitory action...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... structure is locatedin the C-terminal part of the enzyme. The subtilisin-like peptidases are dependent on cal-cium to maintain their stability, and PRK containstwo calcium binding sites, one ... genomicDNA. Nucl Acids Res 21, 2260.39 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteinutilizing the principle of protein-dye binding. ... (http://merops.sanger.ac.uk/).Siezen and Leunissen (1997) subdivided the subtilisin-like serine peptidases or subtilases into six families basedon sequence homology, where the subtilisin and protein-ase K are examples of...

Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx

... assumed to participate in the metabolism of nucleotides. In this context, the assertion thatpyridoxine may be the precursor of the pyrimidine moiety of thiamin in yeast is intriguing [27]. In fungi, ... defect. The relationship of all of these genes remains to be clariﬁed.Expression and puriﬁcation of Sno1p and Snz1pIn light of the similarity in the amino acid sequence of Sno1p to that of the ... Characterization of the products of the genesSNO1 and SNZ1involvedin pyridoxine synthesis inSaccharomyces cerevisiaeYi-Xin Dong, Shinji Sueda, Jun-Ichi Nikawa and Hiroki KondoDepartment...

Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx

... promoters[16,25]. In TATA-less promoters, assembly of the pre-initiation complex relies on binding of multiple generaltranscription factors, such as SP-1, in proximity to the transcription initiation site ... heterologousdesensitization of its signaling in response to the potentanti-aggregatory ⁄ vasodilatory autocoids prostaglandin(PG) I 2(prostacyclin) and nitric oxide (NO) throughmechanisms involving direct ... site (ATG, designated +1) ispresented in Fig. 1. In order to gain further insightsinto the modes of regulation of TPb, the aim of the current study was to map the minimal transcriptionalunit...

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... detail in both the experimental procedures(K i of cyanidin, bilirubin, biliverdin, section B) and in Table 2 (Kd of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin3-glucoside, ... wasassayed to track the progress of the antibody-inducedinhibition. Figure 5A shows that increasing bilirubinconcentrations more and more retarded the progress of activity inhibition. The inhibition ... added to the medium 5 sbefore the addition of the vesicles. The inhibitors were:52.4 lm cyanidin 3-glucoside; 24.6 and 41 lm cyanidin;100 nm bilirubin and 100 nm biliverdin. Under the condi-tions...

Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc

... substrate -binding cleft. It is obvious that elucidation of the 3D structure of PelB would give more insight into structural organiza-tion of the binding site.T. maritima contains at least two evident pectinolyticenzymes. ... oligoGalpA molecules seems to be inconﬂict with its cytoplasmic character and may perhapsbe due to conformational changes in the substrate,thereby facilitating binding to the substrate -binding cleft. ... nature, pectin-degrading(hyper)thermophiles have received relatively littleattention [14–19]. Considering their thermostability and activity, as well as their slightly acidic pH opti-mum, galacturonases...

Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx

... effective inhibition of binding of ICAM-4 positive red cells by anti -ICAM-4 antibodies, indicate a major role for ICAM-4 in binding of red cells to the I domains. The efﬁcient inhibition of the Fig. ... in binding of ICAM-4 and the other two ICAMs for the I domains. Many of these mAbs displayed similar inhibitory proﬁles inassays investigating the effects of the mAbs on the interaction of ICAM ... 270) 1723 Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins Eveliina Ihanus1, Liisa Uotila1, Anne Toivanen1, Michael Stefanidakis1,...

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